Histone H4 [Dimethyl Lys20] Antibody

Images

 
Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - WB analysis of Histone H4K20me2 in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi ...read more
Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per ...read more
Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - WB detection of H4K20me2 in HeLa histone preps.
Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Dot blot analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.
Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]

Product Details

Summary
Reactivity Hu, Mu, Ce, Rt, Ch, Dr, Pl, XpSpecies Glossary
Applications WB, ChIP, DB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.53 mg/ml

Histone H4 [Dimethyl Lys20] Antibody Summary

Immunogen
Synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot P62805].
Modification
Dimethyl Lys20
Localization
Nucleus. Chromosome.
Marker
H3K20me2
Predicted Species
Rat (100%), Drosophila (100%), Chicken (100%), Xenopus (100%), Plant (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Chromatin Immunoprecipitation 2-5 ug per million cells
  • Dot Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50
Application Notes
This Histone H4 K20me2 antibody is useful for ChIP, Immunocytochemistry/Immunofluorescence, Dot blot, and Western blot where a band is seen ~13kDa.
Positive Control
GAPDH primer (NBP1-71650)
RPL30 primer (NBP1-71651)
MYOD1 primer (NBP1-71652)
AFM primer (NBP1-71653)
alpha Satellite repeat primer (NBP1-71654)
Satellite2 repeat primer (NBP1-71655)
Control Peptide
Histone H4 Peptide (NB21-2089PEP)

Reactivity Notes

Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.53 mg/ml
Purity
Immunogen affinity purified

Notes

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

Alternate Names for Histone H4 [Dimethyl Lys20] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K20Me2
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Background

When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NB21-2089). (Showing 1 - 2 of 2 FAQ).

  1. We have a question about the ChIP result graphs obtained by Epi-Plus antibodies. I am sorry that I could not interpret the results. Could you tell us the interpretation of the results of NB21-2088 and NB21-2089 for example?
    • The graphs for NB21-2088 and NB21-2089 show the relative amount of DNA for each listed target that was co-precipitated with chromatin. Briefly, cell lysates were incubated with magnetic beads and antibodies for specific histone modifications. The beads and antibody will isolate only those histone complexes that have the specific modification (for example, Dimethyl Lys20 for NB21-2089). These histone complexes are also bound to DNA, therefore any DNA sequences that are associated with these complexes will also be isolated. Once the histones/DNA are immuoprecipitated, the DNA is extracted and qPCR is performed to measure the relative abundance of certain genes. For NB21-2089, the graph shows that genes whose protein is highly expressed in HeLa cells are co-precipitated with NB21-2089 much LESS than genes that are silent in these cell types. This is expected because the modification that NB21-2089 targets is associated with gene silencing, therefore you would expect less GAPDH signal, and more MYOD1 signal (which is not expressed in HeLa cells).
  2. I am wondering what is the immunogen peptide sequence of H4K20me2 Antibody NB21-2089
    • The immunogen is Synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot P62805]. The exact sequence is considered proprietary.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB21-2089AF405
Alexa Fluor 488 Labeled NB21-2089AF488
Alexa Fluor 647 Labeled NB21-2089AF647
Alexa Fluor 700 Labeled NB21-2089AF700
Biotin Labeled NB21-2089B
DyLight 405 Labeled NB21-2089V
DyLight 488 Labeled NB21-2089G
DyLight 550 Labeled NB21-2089R
DyLight 650 Labeled NB21-2089C
FITC Labeled NB21-2089F
HRP Labeled NB21-2089H

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Blogs on Histone H4.

H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4
Entrez
Uniprot