GAPDH primer

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Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input ...read more
Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ChIP

Order Details

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 0.5 uM
Application Notes
This GAPDH primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 165 base pairs.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 uM in TE buffer.
Preservative
No Preservative

Notes

Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). The Tm for this primer set is between 57.2-63.3 degrees C.

Background

Primers are designed to amplify DNA in exon 1 of the human GAPDH gene locus. GAPDH is an actively transcribed housekeeping gene with significant expression in all cell types. GAPDH is associated with modified histones targeted to active genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

ChIP Video Protocol
ChIP Webinar

FAQs for GAPDH primer (NBP1-71650). (Showing 1 - 1 of 1 FAQ).

  1. Do you have a Tm available for this primer set?
    • The Tm for our GAPDH primer set is between 57.2-63.3 degrees C.

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