GAPDH primer

Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).

Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.

• Reactivity: Hu
• Applications: ChIP

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 0.5 uM
• Application Notes: This GAPDH primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 165 base pairs.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 uM in TE buffer.
• Preservative: No Preservative

Notes

Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). The Tm for this primer set is between 57.2-63.3 degrees C.

Background

Primers are designed to amplify DNA in exon 1 of the human GAPDH gene locus. GAPDH is an actively transcribed housekeeping gene with significant expression in all cell types. GAPDH is associated with modified histones targeted to active genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

FAQs for GAPDH primer (NBP1-71650). (Showing 1 - 1 of 1 FAQ).

1. Do you have a Tm available for this primer set?
   • The Tm for our GAPDH primer set is between 57.2-63.3 degrees C.