Satellite2 repeat primer

Images

 
Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to ...read more
Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ChIP

Order Details

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 0.5 uM
Application Notes
This SAT2 primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 80 base pairs.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 uM in TE buffer.
Preservative
No Preservative

Notes

Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). The Tm for this primer set is between 52.4-53.7 degrees C.

Background

Primers are designed to amplify DNA from human Sat2 repeats in chromosome 1. Sat2 repeats are found in centromeric regions of chromosomes and associated with heterochromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

ChIP Video Protocol
ChIP Webinar

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Satellite2 repeat primer NBP1-71655

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