SETD8 Antibody

Images

 
Western Blot: SETD8 Antibody [NBP1-91269] - Analysis of SETD8 in whole cell lysates of mouse proliferating neural progenitors (P) and differentiating neural stem cell (D) using anti-SETD8 antibody. Image from verified ...read more
Western Blot: SETD8 Antibody [NBP1-91269] - Western blot analysis of SETD8 in Lane 1. SETD8 overexpression vector containing C-terminal DDK tag. Lane 2. Empty vector.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF (-), IHC (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.15 mg/ml

SETD8 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]
Localization
Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SETD8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This SETD8 antibody is useful for Western Blot where a band is seen ~42 kDa representing SETD8 protein. This antibody is not applicable for Immunocytochemistry or Immunohistochemistry.
Positive Control
SETD8 Lysate (NBL1-15870)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-91269 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.15 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SETD8 Antibody

  • H4-K20-HMTase SETD8
  • H4K20-specific histone methyltransferase splice variant Set8b
  • Histone-lysine N-methyltransferase SETD8
  • KMT5A SET domain-containing protein 8
  • KMT5A
  • Lysine N-methyltransferase 5A
  • PR/SET domain containing protein 8
  • PR/SET domain-containing protein 07
  • PR/SET07
  • PRSET7
  • PR-Set7EC 2.1.1.43
  • SET domain containing (lysine methyltransferase) 8
  • SET07SET8H4-K20-specific histone methyltransferase
  • SET8

Background

SETD8 (KMT5A, SET8, PR-Set7, SET domain containing (lysine methyltransferase) 8) is a methyltransferase that appears to be involved in cell cycle regulation; primarily during G2/M phase. A recent study has shown that KMT5A is regulated by phosphorylation at Ser 29 by the cyclin-dependent kinase 1/cyclinB complex from prophase through early anaphase following accumulation of H4K20me1. While the phosphorylation did not alter KMT5A methyltransferase activity, it did cause the removal of KMT5A from mitotic chromosomes. This study also showed that dephosphorylation of Ser 29 in KMT5A during late mitosis is vital to proper mitotic progression. If Ser 29 is not dephosphorylated at the proper time, a delay between metaphase and anaphase occurs (PMID: 20966048). Another study showed that KMT5A binds to the H4 N-terminal tail, consequently blocking the acetylation of lysine residues K5, K8 and K12 of histone H4 during G1, which can inhibit DNA replication (PMID: 18418072).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SETD8 Antibody (NBP1-91269) (0)

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Reviews for SETD8 Antibody (NBP1-91269) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-91269:
Filter by Applications
WB
(2)
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Human
(1)
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot SETD8 NBP1-91269
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reviewed by:
Anonymous
WB Mouse 06/09/2016
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate of mouse neural progenitors proliferating and differentiated for 24 hr.
SpeciesMouse

Blocking

Blocking Details5% non fat dried milk in TBS-T

Primary Anitbody

Dilution Ratio1:1,000 in 3% BSA in TBS, 4 degree overnight incubation

Secondary Antibody

Secondary DescriptionGoat anti rabbit-HRP
Secondary Manufacturer Cat#Thermo 31460
Secondary Concentration1:10,000

Details

Detection NotesBlot was incubated with ECL-femto (Pierce) for 2 min. X-ray film was overlaid in dark and processed through Kodak x-omat.
Western Blot SETD8 NBP1-91269
Enlarge
reviewed by:
Anonymous
WB Human 10/09/2014
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from HCT116 cells grown in DMEM medium
SpeciesHuman

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:1000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30sec

Product General Protocols

View specific protocols for SETD8 Antibody (NBP1-91269): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SETD8 Antibody (NBP1-91269) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-91269AF405
Alexa Fluor 488 Labeled NBP1-91269AF488
Alexa Fluor 647 Labeled NBP1-91269AF647
Alexa Fluor 700 Labeled NBP1-91269AF700
Biotin Labeled NBP1-91269B
DyLight 405 Labeled NBP1-91269V
DyLight 488 Labeled NBP1-91269G
DyLight 550 Labeled NBP1-91269R
DyLight 650 Labeled NBP1-91269C
FITC Labeled NBP1-91269F
HRP Labeled NBP1-91269H

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Recent Reviews

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5
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0
2
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1
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Anonymous
06/09/2016
Application: WB
Species: Mouse

Anonymous
10/09/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SETD8
Entrez
Uniprot