SETD8 Antibody

Western Blot: SETD8 Antibody [NBP1-91269] - Analysis of SETD8 in whole cell lysates of mouse proliferating neural progenitors (P) and differentiating neural stem cell (D) using anti-SETD8 antibody. Image from verified customer review.

Western Blot: SETD8 Antibody [NBP1-91269] - Western blot analysis of SETD8 in Lane 1. SETD8 overexpression vector containing C-terminal DDK tag. Lane 2. Empty vector.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF (-), IHC (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.15 mg/ml

SETD8 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human SETD8 protein (between residues 100-200) [UniProt Q9NQR1]
• Localization: Nucleus. Chromosome. Note: Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SETD8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This SETD8 antibody is useful for Western Blot where a band is seen ~42 kDa representing SETD8 protein. This antibody is not applicable for Immunocytochemistry or Immunohistochemistry.
• Positive Control:

  SETD8 Lysate (NBL1-15870)

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.15 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SETD8 Antibody

• H4-K20-HMTase SETD8
• H4K20-specific histone methyltransferase splice variant Set8b
• Histone-lysine N-methyltransferase SETD8
• KMT5A SET domain-containing protein 8
• KMT5A
• Lysine N-methyltransferase 5A
• PR/SET domain containing protein 8
• PR/SET domain-containing protein 07
• PR/SET07
• PRSET7
• PR-Set7EC 2.1.1.43
• SET domain containing (lysine methyltransferase) 8
• SET07SET8H4-K20-specific histone methyltransferase
• SET8

Background

SETD8 (KMT5A, SET8, PR-Set7, SET domain containing (lysine methyltransferase) 8) is a methyltransferase that appears to be involved in cell cycle regulation; primarily during G2/M phase. A recent study has shown that KMT5A is regulated by phosphorylation at Ser 29 by the cyclin-dependent kinase 1/cyclinB complex from prophase through early anaphase following accumulation of H4K20me1. While the phosphorylation did not alter KMT5A methyltransferase activity, it did cause the removal of KMT5A from mitotic chromosomes. This study also showed that dephosphorylation of Ser 29 in KMT5A during late mitosis is vital to proper mitotic progression. If Ser 29 is not dephosphorylated at the proper time, a delay between metaphase and anaphase occurs (PMID: 20966048). Another study showed that KMT5A binds to the H4 N-terminal tail, consequently blocking the acetylation of lysine residues K5, K8 and K12 of histone H4 during G1, which can inhibit DNA replication (PMID: 18418072).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for SETD8 Antibody (NBP1-91269) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous WB Mouse 06/09/2016

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate of mouse neural progenitors proliferating and differentiated for 24 hr.
• Species: Mouse

Blocking

• Blocking Details: 5% non fat dried milk in TBS-T

Primary Anitbody

• Dilution Ratio: 1:1,000 in 3% BSA in TBS, 4 degree overnight incubation

Secondary Antibody

• Secondary Description: Goat anti rabbit-HRP
• Secondary Manufacturer Cat#: Thermo 31460
• Secondary Concentration: 1:10,000

Details

• Detection Notes: Blot was incubated with ECL-femto (Pierce) for 2 min. X-ray film was overlaid in dark and processed through Kodak x-omat.

reviewed by: Anonymous WB Human 10/09/2014

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from HCT116 cells grown in DMEM medium
• Species: Human

Blocking

• Blocking Details: Non-fat dry milk (5%) in PBS

Primary Anitbody

• Dilution Ratio: 1:1000 in PBS at RT for 3 hours

Secondary Antibody

• Secondary Description: Anti rabbit IgG (HRP-conjugated)
• Secondary Manufacturer Cat#: ab6721 (Abcam)
• Secondary Concentration: 1:20K

Details

• Detection Notes: Bio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 30sec

Bioinformatics

• Gene Symbol: SETD8
• Entrez:
  • 387893(Human)
• Uniprot:
  • Q9NQR1(Human)