alpha Satellite repeat primer

Images

 
Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.
Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ChIP

Order Details

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 0.5 uM
Application Notes
This alpha SAT primer is useful for PCR in chromatin precipitation. Amplicon size of this primer set is 150 base pairs.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 uM in TE buffer.
Preservative
No Preservative

Notes

Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). Tm for this primer set is between 53.4-54.0 degrees C.

Background

Primers are designed to amplify DNA from human alpha satellite repeats in chromosome 1. Alpha satellite repeats are found in centromeric regions of chromosomes and associated with heterochromatin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

ChIP Video Protocol
ChIP Webinar

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