| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-30091SS | |||
| Azide and BSA Free | NBP2-80771 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5215-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-2044V | |||
| FITC | NB21-2044F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2044SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9549-SP | |||
![Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti-Histone H3 Antibody (NB500-171) used at 1:20,000 and Anti-Histone H4 used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12--ac-Lys16--ac-Lys8--ac-Lys5]-Antibody-Western-Blot-NBP2-16848-img0015.jpg "Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts described in (a). (c) Histone levels in dermal fibroblasts isolated from human neonatal (age 0, donors a and b) and adult donors. Anti-Histone H3 Antibody (NB500-171) used at 1:20,000 and Anti-Histone H4 used at 1:10,000. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1038/s41598-020-59163-4) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-16848] - Human breast cancer, using antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-16848-img0010.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - WB for total histone in MCF-7 and HCT116 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0008.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 15% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0012.jpg)
![Western Blot: Histone H4 Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-16848-img0013.jpg)
![Dot Blot: Histone H4 Antibody [NBP2-16848] - Peptide samples (0.1 ug) were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17) antibody Histone H4 at different dilution as indicated. A: Peptide samples of Histone H4 (acetyl Lys6, Lys9, Lys13, Lys17). B: Peptide samples of Histone H4](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Dot-Blot-NBP2-16848-img0007.jpg)
![Western Blot: Histone H4 [ac Lys12, ac Lys16, ac Lys8, ac Lys5] Antibody [NBP2-16848] - Sample (30 ug of whole cell lysate) A: HeLa 15% SDS PAGE NBP2-16848 diluted at 1:10000](http://images.novusbio.com/fullsize/nbp2-16848_rabbit-polyclonal-histone-h4-ac-lys12-ac-lys16-ac-lys8-ac-lys5-antibody-91020242021955.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NB21-2044-img0006.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2044-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NB21-2044-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NB21-2044-img0004.jpg)
![Histone H4 [ac Lys8] Antibody [NB21-2044]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-NB21-2044-img0008.jpg)
![Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0008.jpg "Western Blot: Histone H4 Antibody [NBP2-42856] - Various tissue extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Rat brain stem. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0004.jpg)
![Western Blot: Histone H4 Antibody [NBP2-42856] - Analysis of various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 antibody diluted at a dilution of 1:5000.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Western-Blot-NBP2-42856-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse fore brain. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 Antibody [NBP2-42856] - Mouse muscle. Histone H4 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H4-Antibody-Immunohistochemistry-Paraffin-NBP2-42856-img0003.jpg)
![Histone H4 antibody detects Histone H4 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 protein stained by Histone H4 antibody (NBP2-42856) diluted at 1:5000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:2000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42856_rabbit-polyclonal-histone-h4-antibody-immunocytochemistry-immunofluorescence-261120251844350.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-42862-img0007.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000")
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0005.jpg)
![Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (NBP2-42862) diluted at 1:500.Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-42862_rabbit-polyclonal-histone-h4-trimethyl-lys20-antibody-immunocytochemistry-immunofluorescence-26112025182906.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Western-Blot-NBP2-42858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse brain. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse kidney. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0002.jpg)
![Histone H4K16ac (Acetyl Lys16) antibody [GT1271] detects Histone H4K16ac (Acetyl Lys16) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunohistochemistry-paraffin-261120251828104.jpg)
![Histone H4K16ac (Acetyl Lys16) antibody [GT1271] detects Histone H4K16ac (Acetyl Lys16) protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunohistochemistry-paraffin-261120251835434.jpg)
![Histone H4 (acetyl Lys16) antibody [GT1271] detects Histone H4 (acetyl Lys16) protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [GT1271] (NBP2-42858) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-42858_mouse-monoclonal-histone-h4-ac-lys16-antibody-1271-immunocytochemistry-immunofluorescence-261120251848237.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys8) antibody (NBP3-13539) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Western-Blot-NBP3-13539-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys8) antibody (NBP3-13539) diluted at 1:1000.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:400. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunocytochemistry-Immunofluorescence-NBP3-13539-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis. Sample: Paraffin-embedded human ovary. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody (GT478) [NBP3-13539] - Histone H4 (acetyl Lys8) antibody [GT478] detects Histone H4 (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Histone H4 (acetyl Lys8) antibody [GT478] (NBP3-13539) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys8-Antibody-GT478-Immunohistochemistry-Paraffin-NBP3-13539-img0004.jpg)
![Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Western-Blot-NBP2-59266-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K12ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys12] Antibody [NBP2-59266] - HeLa cells were stained with the antibody against H4K12ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K12ac antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Immunofluorescence-NBP2-59266-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody [NBP2-59266] - ChIP assays were performed using human HeLa cells, the antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with the ChIP-seq kit on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active GAPDH and EIF4A2 genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Chromatin-Immunoprecipitation-NBP2-59266-img0003.jpg)
![ELISA: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:14,300.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-ELISA-NBP2-59266-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys12] Antibody [NBP2-59266] - To test the cross reactivity of the antibody against H4K12ac, a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows the antibody is specific for the K12 acetylation with some slight cross reaction with K8ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys12]-Antibody-Dot-Blot-NBP2-59266-img0007.jpg)
