Histone H3 Antibody

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Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.
Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ...read more
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.
Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.
Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.
Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Xp, Ye, Ce, Ch, Dr, PlSpecies Glossary
Applications WB, ChIP, IB, ICC/IF, IHC, IHC-P, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

Histone H3 Antibody Summary

Immunogen
This Histone H3 antibody was raised against a synthetic peptide made to an C-terminal portion of the human Histone H3 protein (between residues 100-136) [UniProt P68431]
Localization
Nuclear
Predicted Species
Plant (100%), C. elegans (100%), Chicken (100%), Drosophila (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST2H3C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:4000
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Single Cell Western
Application Notes
This Histone H3 antibody is useful for Western Blot and IHC-paraffin embedded sections. In Western Blot, a band is seen ~15 kDa. In IHC-P, nuclear staining was observed in human, mouse, and rat tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24048589). Use in immunoblotting reported in scientific literature (PMID: 24048589).
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 5 Reviews rated 5
using
NB500-171 in the following applications:

Publications
Read Publications using
NB500-171 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • KD
    1 publication
  • WB
    11 publications

Reactivity Notes

Predicted to react with many species based on 100% sequence homology including C. elegans, chicken, drosophila, and plant. Xenopus reactivity reported in scientific literature (PMID: 24048589)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3 Antibody

  • H3 histone, family 3A
  • H3
  • H3.3A
  • H3.3B
  • H3F2
  • H3F3
  • H3F3A
  • H3F3B
  • H3FM
  • HIST2H3C
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K,...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H3 Antibody (NB500-171)(12)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, C. elegans, Xenopus.

We have publications tested in 4 applications: ICC/IF, IHC, KD, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
KD
(1)
WB
(11)
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Filter By Species
Human
(4)
Mouse
(3)
Rat
(1)
C. elegans
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB500-171 Applications Species
Zhang YX, Akumuo RC, Espana RA et al. The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory Neuropharmacology Aug 27 2018 [PMID: 30165076] (WB, Mouse) WB Mouse
Xu Y, Ning S, Wei Z et al. 53BP1 and BRCA1 control pathway choice for stalled replication restart Elife. 2017 Nov 06 [PMID: 29106372] (WB) WB
Gulchina Y, Xu SJ, Snyder MA et al. Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia. J. Neurochem. 2017 Jun 19 [PMID: 28628228] (WB) WB
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014 [PMID: 25587023] (WB, Human) WB Human
Petridou NI, Stylianou P, Skourides PA. A dominant-negative provides new insights into FAK regulation and function in early embryonic morphogenesis. Development. 2013 Oct [PMID: 24048589] (ICC/IF, IHC, Xenopus) ICC/IF, IHC Xenopus
Kong HK, Wong MH, Chan HM, Lo SC. Chronic Exposure of Adult Rats to Low Doses of Methylmercury Induced a State of Metabolic Deficit in the Somatosensory Cortex. J Proteome Res. 2013 Aug 28 [PMID: 23984759] (WB, Rat) WB Rat
Macfarlan TS, Gifford WD, Driscoll S et al. Embryonic stem cell potency fluctuates with endogenous retrovirus activity Nature 2012 Jun 13 [PMID: 22722858] (WB, Mouse) WB Mouse
Liu R, Lei JX, Luo C et al. Increased EID1 nuclear translocation impairs synaptic plasticity and memory function associated with pathogenesis of Alzheimer's disease Neurobiology of Disease 2012 Mar 12 [PMID: 22186421] (WB, Mouse) WB Mouse
Wibisono P, Liu Y, Sun J A novel in vitro Caenorhabditis elegans transcription system bioRxiv Jun 24 2020 (WB, C. elegans) WB C. elegans
Auzmendi-Iriarte J, Saenz-Antonanzas A, Mikelez-Alonso I et al. Characterization of a new small-molecule inhibitor of HDAC6 in glioblastoma Cell Death Dis Jun 2 2020 [PMID: 32488056] (WB, Human) WB Human
Show All 12 Publications.

Reviews for Histone H3 Antibody (NB500-171) (5) 55

Average Rating: 5
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using NB500-171:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
Western Blot Histone H3 NB500-171
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reviewed by:
Anonymous
WB Human 05/18/2020
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Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotA-3
Western Blot Histone H3 NB500-171
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reviewed by:
Anonymous
WB Human 10/22/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotA-3
Western Blot Histone H3 NB500-171
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reviewed by:
Anonymous
WB Human 12/27/2016
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 cells (cytoplasmic and nuclear fractions)
SpeciesHuman
Western Blot Histone H3 NB500-171
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reviewed by:
Anony- mous
WB Human 06/13/2014
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsWestern blot analysis of extracts from HeLa, COS, C6 and K562 cells using Histone H3 antibody (NB500-171, 1:100).
FileView PDF
Western Blot Histone H3 NB500-171
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reviewed by:
Anonymous
WB Human 06/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell extract from MCF-7 and HCT-116 cells
SpeciesHuman
CommentsThis histone antibody works very good even with total cell lyaste. A few others give a lot of background with total cell lysate and a clean blot with acid extracted histones. But this one works good even with total cell lyaste and the blot is even cleaner with extracted histones.

Blocking

Blocking DetailsNon-fat dry milk (5%) in PBS

Primary Anitbody

Dilution Ratio1:2000 in PBS at RT for 3 hours

Secondary Antibody

Secondary DescriptionAnti rabbit IgG (HRP-conjugated)
Secondary Manufacturer Cat#ab6721 (Abcam)
Secondary Concentration1:20K

Details

Detection NotesBio-Rad Immun-Star HRP Substrate was used with X-ray film, Exposure time: 5sec

Comments

CommentsThis histone antibody works very good even with total cell lyaste. A few others give a lot of background with total cell lysate and a clean blot with acid extracted histones. But this one works good even with total cell lyaste and the blot is even cleaner with extracted histones.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB500-171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB500-171B
DyLight 405 NB500-171V
FITC NB500-171F

Additional Histone H3 Products

Bioinformatics Tool for Histone H3 Antibody (NB500-171)

Discover related pathways, diseases and genes to Histone H3 Antibody (NB500-171). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Histone H3 Antibody (NB500-171)

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Blogs on Histone H3.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Recent Reviews

5
5
5
4
0
3
0
2
0
1
0

Anonymous
05/18/2020
Application: WB
Species: Human

Anonymous
10/22/2018
Application: WB
Species: Human

Anonymous
12/27/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol HIST2H3C
Entrez
Uniprot