Histone H3 Antibody - BSA Free

Images

 
Western Blot: Histone H3 Antibody [NB500-171] - Analysis of HeLa, COS, C6, and K562 cell lysate using histone H3 antibody [NB500-171] antibody at 1:100. Observed molecular weight is ~17 kDa.
Chromatin Immunoprecipitation: Histone H3 Antibody [NB500-171] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NB500-171 and 25 uL of magnetic IgG beads, using standard ...read more
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse testis.
Western Blot: Histone H3 Antibody [NB500-171] - MDA-MB-231 cells were treated with vehicle (V) or paclitaxel (P). Cytosolic and nuclear lysates were prepared, and immunoblot assay was performed. WB image submitted by a ...read more
Immunohistochemistry: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.
Western Blot: Histone H3 Antibody [NB500-171] - Reduced histone levels in senescent cells is induced in vitro by different means, and in vivo from aged donors. (b) Western immunoblot analyses of histones in fibroblasts ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - Immunofluorescence staining to detect histone H3 in human blood. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Analysis of Histone H3 antibody on Mouse pancreas tissue. Histone H3 antibody dilution 1:200. Image from verified customer review.
Western Blot: Histone H3 Antibody [NB500-171] - (1) HeLa, (2) S. cerevisiae whole cell lysates, (3) Histones purified from HeLa cells. Theoretical molecular weight is ~15 kDa.
Western Blot: Histone H3 Antibody [NB500-171] - Total histone H3 levels in MCF-7 and HCT-116 cells. Observed molecular weight is ~17 kDa. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody [NB500-171] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat pancreas.
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE rat colon.
Immunohistochemistry-Paraffin: Histone H3 Antibody [NB500-171] - Immunoperoxidase staining of Anti-Histone H3 in FFPE mouse brain.
Immunohistochemical detection of HIF-1 alpha protein in human tissues. The nature of the tissue is indicated on top of each figure. Original magnifications are as follows: A, ×400; B, ×400; C, ×100; D, ×100; E, ...read more
UFL1 protein accumulates at DSBs through the MRN complex. a U2OS cells stably expressing UFL1 Tet-on shRNA were treated with doxycycline (Dox) for 3–5 days, and then treated with or without 2 Gy IR. After 30 min, ...read more
IL25 upregulated GLI1 by inhibiting p-AMPK. (A) HT-29 cells were treated with cycloheximide (CHX, 50 μg/ml) for the indicated time, and cell lysates were analyzed by Western blotting with the indicated antibodies. (B) ...read more
A TLR3 agonist polyinosinic‐polycytidylic acid (poly(I:C)) induced autophagy in cultured cardiomyocytes through a TRIF‐dependent pathway. (A) Poly(I:C) increased autophagy markers in cultured H9c2 rat ventricular ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, I, Xp, Ye, Ce, Ch, Dr, PlSpecies Glossary
Applications WB, ICC/IF, IHC, WB, ChIP, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Histone H3 Antibody - BSA Free Summary

Immunogen
This Histone H3 antibody was raised against a synthetic peptide made to an C-terminal portion of the human Histone H3 protein (between residues 100-136) [UniProt P68431]
Localization
Nuclear
Predicted Species
Plant (100%), C. elegans (100%), Chicken (100%), Drosophila (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H3C14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoblotting reported in scientific literature (PMID 24048589)
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 24048589)
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:100-1:300
  • Single Cell Western
  • Western Blot 1:1000-1:4000
Application Notes
In Western Blot, a band is seen ~15 kDa. In IHC-P, nuclear staining was observed in human, mouse, and rat tissues. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 9 Reviews rated 4.9
using
NB500-171 in the following applications:

Publications
Read Publications using
NB500-171 in the following applications:

Reactivity Notes

Predicted to react with many species based on 100% sequence homology including C. elegans, chicken, drosophila, and plant. Xenopus reactivity reported in scientific literature (PMID: 24048589). Insect (Aedes aegypti) reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H3 Antibody - BSA Free

  • H3 histone, family 3A
  • H3
  • H3.3A
  • H3.3B
  • H3F2
  • H3F3
  • H3F3A
  • H3F3B
  • H3FM
  • HIST2H3C
  • Histone H3
  • histone H3.3
  • MGC87782
  • MGC87783

Background

Chromatin is an arrangement of DNA and proteins which form chromosomes. Chromatin is formed from nucleosomes, which are comprised of a set of four histone proteins (H2A, H2B, H3, H4) wrapped with DNA. Chromatin is very dynamic, where post-translational modifications can regulate DNA to be copied, transcribed, or repaired.

Histones are nuclear proteins responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Changes in chromatin structure play a large role in the regulation of transcription. The chromatin fibers are compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. Posttranslational modifications such as acetylation of core histones regulates gene expression, thus altering protein function and regulation (1). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 and have a theoretical molecular weight of 15 kDa. Acetylation at lysine 9 and 14 appears to control histone deposition, chromatin assembly and active transcription. Methylation of arginine residues within histone H3 has also been linked to transcription regulation. Histone H3 has been linked to various types of cancer as a biomarker through the aberrant expression of histone deacetylase (HDAC) enzymes and changes to chromatins (2-4).

References

1. Zhang, Y. X., Akumuo, R. C., Espana, R. A., Yan, C. X., Gao, W. J., & Li, Y. C. (2018). The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory. Neuropharmacology, 141, 113-125. doi:10.1016/j.neuropharm.2018.08.030

2. Nandakumar, V., Hansen, N., Glenn, H. L., Han, J. H., Helland, S., Hernandez, K, ...Meldrum, D. R. (2016). Vorinostat differentially alters 3D nuclear structure of cancer and non-cancerous esophageal cells. Sci Rep, 6, 30593. doi:10.1038/srep30593

3. Zhou, M., Li, Y., Lin, S., Chen, Y., Qian, Y., Zhao, Z., & Fan, H. (2019). H3K9me3, H3K36me3, and H4K20me3 Expression Correlates with Patient Outcome in Esophageal Squamous Cell Carcinoma as Epigenetic Markers. Dig Dis Sci, 64(8), 2147-2157. doi:10.1007/s10620-019-05529-2

4. Li, Y., Guo, D., Sun, R., Chen, P., Qian, Q., & Fan, H. (2019). Methylation Patterns of Lys9 and Lys27 on Histone H3 Correlate with Patient Outcome in Gastric Cancer. Dig Dis Sci, 64(2), 439-446. doi:10.1007/s10620-018-5341-8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Histone H3 Antibody (NB500-171)(27)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 5 applications: Chemotaxis, ICC/IF, IF/IHC, KD, WB.


Filter By Application
Chemotaxis
(2)
ICC/IF
(2)
IF/IHC
(1)
KD
(1)
WB
(16)
All Applications
Filter By Species
Human
(7)
Mouse
(4)
Rat
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB500-171 Applications Species
Chellini L, Scarfò M, Bonvissuto D et al. The DNA/RNA helicase DHX9 orchestrates the KDM2B-mediated transcriptional regulation of YAP1 in Ewing sarcoma Oncogene 2023-11-28 [PMID: 38017132]
Verdile V, Palombo R, Ferrante G et al. Dysregulation of alternative splicing underlies synaptic defects in familial Amyotrophic Lateral Sclerosis Progress in neurobiology 2023-09-20 [PMID: 37739207] (WB, Human) WB Human
Malnar ?rnigoj M, ?er?ek U, Yin X et al. Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA Nat Commun 2023-09-16 [PMID: 37717009] (ICC/IF) ICC/IF
Fan M, Yang K, Wang X et al. Lactate promotes endothelial-to-mesenchymal transition via Snail1 lactylation after myocardial infarction Science advances 2023-02-03 [PMID: 36735787] (WB, Mouse) WB Mouse
Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (Chemotaxis, Human)

Details:
Citation using the DyLight 405 version of this antibody.
Chemotaxis Human
Ait Saada A, Costa AB, Lobachev KS. Characterization of canavanine-resistance of cat1 and vhc1 deletions and a dominant any1 mutation in fission yeast PLOS ONE 2022-05-31 [PMID: 35639710] (WB) WB
Chellini L, Pieraccioli M, Sette C, Paronetto MP. The DNA/RNA helicase DHX9 contributes to the transcriptional program of the androgen receptor in prostate cancer Journal of Experimental & Clinical Cancer Research 2022-12-01 [PMID: 35590370] (WB) WB
Kabacik S, Lowe D, Cohen H et al. Isolation of five different primary cell types from a single sample of human skin STAR Protocols 2022-06-01 [PMID: 35586317] (WB) WB
Decker CJ, Burke JM, Mulvaney PK, Parker R RNA is required for the integrity of multiple nuclear and cytoplasmic membrane-less RNP granules The EMBO journal 2022-05-02 [PMID: 35355287] (WB) WB
Palombo R, Paronetto MP pncCCND1_B Engages an Inhibitory Protein Network to Downregulate CCND1 Expression upon DNA Damage Cancers 2022-03-17 [PMID: 35326688] (Chemotaxis, Human) Chemotaxis Human
Show All 27 Publications.

Reviews for Histone H3 Antibody (NB500-171) (9) 4.99

Average Rating: 4.9
(Based on 9 reviews)
We have 9 reviews tested in 3 species: Human, Mouse, Ae. aegypti.

Reviews using NB500-171:
Filter by Applications
WB
(7)
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(7)
Mouse
(1)
Ae. aegypti
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Histone H3 NB500-171
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5
reviewed by:
Verified Customer
WB Human 01/26/2023
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Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate
SpeciesHuman
LotB-1
Immunohistochemistry-Paraffin Histone H3 NB500-171
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5
reviewed by:
Verified Customer
IHC-P Mouse 02/13/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPancreas tissue
SpeciesMouse
LotA-10

Comments

CommentsHistone H3 antibody dilution 1:200
Immunofluorescence Histone H3 NB500-171
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5
reviewed by:
Verified Customer
IF Human 07/30/2021
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Summary

ApplicationImmunofluorescence
Sample Testedhuman blood
SpeciesHuman
LotA-10
Western Blot Histone H3 NB500-171
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4
reviewed by:
Adeline Williams
WB Ae. aegypti 05/22/2021
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Summary

ApplicationWestern Blot
Sample TestedA549 Nuclear and cytoplasmic, Sample Amount: 30ug,Midgut tissue,Ovary tissue
SpeciesAe. aegypti
LotA-10

Comments

CommentsTo test both anti-histone H3 and anti-beta tubulin as markers for nuclear or cytoplasmic fractions, respectively, in Ae. aegypti mosquitoes, a western blot was performed using midguts or ovaries dissected from mosquitoes 48h post-bloodmeal. The cytoplasmic or nuclear fractions from each tissue type were obtained and protein concentration was quantified by A260/280. Roughly 30ug of tissue was loaded onto SDS/PAGE gels that were either transferred to PVDF membranes or stained by Coomassie. Membranes were blocked in TBST 5% milk for 2h. Both anti-histone H3 and anti-beta tubulin were added 1:1000 in blocking buffer and incubated O/N shaking at 4C. Proteins were detected by Alkaline Phosphatase. In Ae. aegypti ovaries, anti-histone H3 bound to protein roughly 15kDa in the nuclear fraction of ovaries but not the cytoplasmic fraction, while anti-beta-tubulin bound to protein roughly 55kDa in both cytoplasmic and nuclear fractions. In Ae. aegypti midguts, anti-histone H3 bound to 3 proteins between 10-15kDa strongly in the nuclear fraction and faintly in the cytoplasmic fraction (perhaps due to nuclear contamination in the cytoplasmic fraction). anti-beta-tubulin did not react well in midgut fractions. For your reference, protein ladder = pageruler plus prestained ThermoFisher
Western Blot Histone H3 NB500-171
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5
reviewed by:
Verified Customer
WB Human 05/18/2020
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Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotA-3
Western Blot Histone H3 NB500-171
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5
reviewed by:
Verified Customer
WB Human 10/22/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
LotA-3
Western Blot Histone H3 NB500-171
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5
reviewed by:
Verified Customer
WB Human 12/27/2016
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Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 cells (cytoplasmic and nuclear fractions)
SpeciesHuman
Western Blot Histone H3 NB500-171
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5
reviewed by:
Bryan Tinsley
WB Human 06/13/2014
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Summary

ApplicationWestern Blot
SpeciesHuman
Western Blot Histone H3 NB500-171
Enlarge
5
reviewed by:
Verified Customer
WB Human 06/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell extract from MCF-7 and HCT-116 cells
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone H3 Antibody (NB500-171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Histone H3 Products

Research Areas for Histone H3 Antibody (NB500-171)

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Blogs on Histone H3. Showing 1-10 of 11 blog posts - Show all blog posts.

Epigenetics of Depression: How Can Psychological Stress Alter Your DNA?
By Emily Cartwright, PhDHow Can Psychological Stress Alter Your DNA? Traumatic events, work demands, relationship conflicts, and health problems are all examples of psychological stressors that can result in phy...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

Understanding Transcription with RNA Polymerase II
RNA polymerase II is a large 12-subunit complex that synthesizes all mRNAs and several non-coding RNAs in eukaryotic cells. It is a DNA-dependent RNA polymerase enzyme that catalyzes transcription of DNA into RNA based on the four ribonucleoside triph...  Read full blog post.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

EZH2: Epigenetic Regulation Made Easy!
Enhancer of Zeste homolog 2 (EZH2) is the methyltransferase enzyme responsible for trimethylating lysine 27 on histone H3 to produce H3K27Me3. EZH2 is a polycomb group protein that is an essential epigenetic regulator that is often found deregulated i...  Read full blog post.

Understanding the Reasons for Histone H3 K4 Trimethylation (H3K4Me3)
Epigenetic mechanisms allow distinction between the active and inactive compartments of the genome, allowing proper cell lineage and embryogenesis. The trimethylation of Histone 3 at lysine 4 (H3K4Me3) is a common epigenetic histone modification that ...  Read full blog post.

The 'epi-genie' is Out of the Bottle: Functional Histone 3 Variants in Human Disease
Discovery of histone variants using highly specific antibodies has led to the emerging notion that alterations in histone modifications and further changes in chromatin structure are induced by exchange of histone variants. Covalent histone modificati...  Read full blog post.

Showing 1-10 of 11 blog posts - Show all blog posts.
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Recent Reviews

4.9
5
8
4
1
3
0
2
0
1
0

Verified Customer
01/26/2023
Application: WB
Species: Human

Verified Customer
02/13/2022
Application: IHC-P
Species: Mouse

Verified Customer
07/30/2021
Application: IF
Species: Human

Bioinformatics

Gene Symbol H3C14
Entrez
Uniprot