AFM primer


Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input more

Product Details

Applications ChIP

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  • Chromatin Immunoprecipitation 0.5 uM
Application Notes
This AFM primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 101 base pairs.

Reactivity Notes


Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
10 uM in TE buffer.
No Preservative


Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). The Tm for this primer set is between 51.9-52.6 degrees C.


Primers are designed to amplify DNA in intron 1 of the human AFM gene locus. AFM is expressed in the liver and therefore silent in most cell types. AFM is associated with modified histones targeted to repressed genes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

ChIP Video Protocol
ChIP Webinar

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Array NBP1-71653

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