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Sir2.1 Antibody

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 (1 Review)
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Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.
Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.

Product Details

Summary
Reactivity CeSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Sir2.1 Antibody Summary

Immunogen
A full-length recombinant protein to Sir2.1.
Localization
Exclusively nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:3000
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This Sir2.1 antibody is useful for Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot.
Reviewed Applications
Read 1 Review rated 5
using
NB100-1923 in the following applications:
Publications
Read Publications using
NB100-1923 in the following applications:

  • WB
    1 publication

Reactivity Notes

C. elegans.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Sir2.1 Antibody

  • EC 3.5.1
  • EC 3.5.1.-
  • FLJ35621
  • FLJ37491
  • S.cerevisiae, homolog) 2
  • sirtuin 2
  • sirtuin type 2

Background

Sir2 (silent information regulator 2) protein plays a crucial role in regulating the life span of C. elegans by means of caloric restricion. Overexpression of the protein can extend the life span, and is, thus, a regulator of longevity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sir2.1 Antibody (NB100-1923)(2)

We have publications tested in 1 confirmed species: C. elegans.

We have publications tested in 1 application: WB.


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Review for Sir2.1 Antibody (NB100-1923) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-1923:
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(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
 IP Other 12/27/2011
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Summary

ApplicationImmunoprecipitation
Sample TestedC. elegans
SpeciesOther
LotA1
CommentsWorks well on blot with many different ECL preps. Originally, there was quite a lot of background signal with this Ab, mostly from IgG. I was able to greatly mitigate this by using Clean-Blot IP detection reagent (HRP) from Thermo Scientific, which binds native IgG with a much higher affinity versus denatured.
Pub Med ID21909281

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Comments

CommentsWorks well on blot with many different ECL preps. Originally, there was quite a lot of background signal with this Ab, mostly from IgG. I was able to greatly mitigate this by using "Clean-Blot" IP detection reagent (HRP) from Thermo Scientific, which binds native IgG with a much higher affinity versus denatured.

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FAQs for Sir2.1 Antibody (NB100-1923). (Showing 1 - 1 of 1 FAQs).

  1. Our customer would like to double check if the reactivity of NB100-1923 is yeast? Also, would you tell us what species was used in the lanes shown on the Western blot for your product?
    • NB100-1923 is used to detect the C. elegans protein; yeast is not a validated species. The species used in the western blot was C. elegans.

Secondary Antibodies

 

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Recent Reviews

5
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1
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3
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1
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 Anonymous
12/27/2011
Application: IP
Species: Other
read this review

Bioinformatics

Gene Symbol SIRT2
Entrez