Sir2.1 Antibody

Western Blot: Sir2.1 Antibody [NB100-1923] - Western blot using NB100-1923 against His tagged SIR-2.1 full-length protein.

• Reactivity: Ce
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Sir2.1 Antibody Summary

• Immunogen: A full-length recombinant protein to Sir2.1.
• Localization: Exclusively nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIRT2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:3000
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: This Sir2.1 antibody is useful for Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot.

Reactivity Notes

C. elegans.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Sir2.1 Antibody

• EC 3.5.1
• EC 3.5.1.-
• FLJ35621
• FLJ37491
• S.cerevisiae, homolog) 2
• sirtuin 2
• sirtuin type 2

Background

Sir2 (silent information regulator 2) protein plays a crucial role in regulating the life span of C. elegans by means of caloric restricion. Overexpression of the protein can extend the life span, and is, thus, a regulator of longevity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sir2.1 Antibody (NB100-1923)(2)

Publications using NB100-1923

• Fang EF, Scheibye-Knudsen M, Brace LE et al. Defective Mitophagy in XPA via PARP-1 Hyperactivation and NAD(+)/SIRT1 Reduction. Cell. 2014 May 08 [PMID: 24813611] (WB, C. elegans)
• Rizki G, Iwata TN, Li J et al. The Evolutionarily Conserved Longevity Determinants HCF-1 and SIR-2.1/SIRT1 Collaborate to Regulate DAF-16/FOXO. PLoS Genet;7(9). 2011 Sep. [PMID: 21909281]

Review for Sir2.1 Antibody (NB100-1923) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous IP Other 12/27/2011

Summary

• Application: Immunoprecipitation
• Sample Tested: C. elegans
• Species: Other
• Lot: A1
• Comments: Works well on blot with many different ECL preps. Originally, there was quite a lot of background signal with this Ab, mostly from IgG. I was able to greatly mitigate this by using Clean-Blot IP detection reagent (HRP) from Thermo Scientific, which binds native IgG with a much higher affinity versus denatured.
• Pub Med ID: 21909281

Primary Anitbody

• Dilution Ratio: Primary Ab Dilution Ratio: 1:1000

Comments

• Comments: Works well on blot with many different ECL preps. Originally, there was quite a lot of background signal with this Ab, mostly from IgG. I was able to greatly mitigate this by using "Clean-Blot" IP detection reagent (HRP) from Thermo Scientific, which binds native IgG with a much higher affinity versus denatured.

FAQs for Sir2.1 Antibody (NB100-1923). (Showing 1 - 1 of 1 FAQs).

1. Our customer would like to double check if the reactivity of NB100-1923 is yeast? Also, would you tell us what species was used in the lanes shown on the Western blot for your product?
   • NB100-1923 is used to detect the C. elegans protein; yeast is not a validated species. The species used in the western blot was C. elegans.

Bioinformatics

• Gene Symbol: SIRT2
• Entrez:
  • 177924(C. elegans)