MYOD1 primer

Images

 
Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.
Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input ...read more

Product Details

Summary
Applications ChIP

Order Details

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 0.5 uM
Application Notes
This MYOD1 primer is useful for PCR in chromatin precipitation. Amplicon size for this primer set is 112 base pairs.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 uM in TE buffer.
Preservative
No Preservative

Notes

Primer sets come premixed with forward and reverse primers at a concentration of 10 uM. Primers are compatible with all real-time PCR chemistries. Each primer set has been tested and validated by chromatin immunoprecipitation (ChIP). The Tm for this primer set is between 54.4-54.9 degrees C.

Background

Primers are designed to amplify DNA in exon 1 of the human MyoD1 gene locus. MyoD1 is expressed during muscle development and therefore silent in most cell types. MyoD1 is associated with modified histones targeted to repressed genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

ChIP Video Protocol
ChIP Webinar

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Additional MYOD1 primer Products

MYOD1 primer NBP1-71652

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