MYST1 Antibody

Western Blot: MYST1 Antibody [NB100-55251] - Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody NB100-55251 (lot NB100-55251-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot NB100-55251-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Immunohistochemistry-Paraffin: MYST1 Antibody [NB100-55251] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiMOF/MYST1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: MYST1 Antibody [NB100-55251] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

MYST1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human males absent on the first (MYST histone acetyltransferase 1) using the numbering given in entry BAB13924.1 (GeneID 84148).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KAT8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2 -5 ug/mg lysate
  • Western Blot 1:1000 -1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MYST1 Antibody

• FLJ14040
• hMOFhistone acetyltransferase MYST1
• KAT8
• MOFEC 2.3.1.48
• MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
• MYST histone acetyltransferase 1
• MYST-1
• ortholog of Drosophila males absent on the first (MOF)
• probable histone acetyltransferase MYST1

Background

The MYST family of histone acetyltransferases, which includes MYST1, is named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin (Neal et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYST1 Antibody (NB100-55251)(3)

Publications using NB100-55251

• Badeaux AI, Yang Y, Cardenas K et al. Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF. J Biol Chem 2012-01-01 [PMID: 22072714]
• Lu L, Li L, Lv X et al. Modulations of hMOF autoacetylation by SIRT1 regulate hMOF recruitment and activities on the chromatin. Cell Res 2011-08-01 [PMID: 21502975]
• Li X, Corsa CA, Pan PW et al. MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1. Mol Cell Biol 2010-11-01 [PMID: 20837706]

Bioinformatics

• Gene Symbol: KAT8
• Entrez:
  • 84148(Human)
• Uniprot:
  • BAB13924.1(Human)