eIF4E [p Ser209] Antibody - BSA Free

Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898

Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898(left) or the same antibody preincubated with blocking peptide (right).

Immunocytochemistry/ Immunofluorescence: eIF4E [p Ser209] Antibody - BSA Free [NB100-81898] - Immunofluorescence staining of methanol-fixed MCF cells using NB100-81898.

• Reactivity: Hu, Po
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

eIF4E [p Ser209] Antibody - BSA Free Summary

• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human elF4E around the phosphorylation site of serine 209 (S-G-SP-T-T).
• Modification: p Ser209
• Specificity: Detects endogenous levels of elF4E only when phosphorylated at serine 209.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4E
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  eIF4E Overexpression Lysate

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28183860).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4E [p Ser209] Antibody - BSA Free

• CBP
• eIF4E
• eIF-4E
• EIF4E1
• EIF4EL1
• EIF4EL1MGC111573
• eIF-4F 25 kDa subunit
• EIF4F
• eukaryotic translation initiation factor 4E
• eukaryotic translation initiation factor 4E-like 1
• mRNA cap-binding protein

Background

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4E Antibody (NB100-81898)(1)

Publication using NB100-81898

• Chen Y, Zhu H, McCauley SR et al. Diminished satellite cell fusion and S6K1 expression in myotubes derived from skeletal muscle of low birth weight neonatal pigs. Physiol Rep. 2017-02-01 [PMID: 28183860] (WB, Porcine)

Bioinformatics

• Gene Symbol: EIF4E
• Entrez:
  • 1977(Human)
  • 13684(Mouse)
  • 117045(Rat)
• Uniprot:
  • P06730()