MEF2C Antibody (OTI1H5)

Western Blot: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C.

Immunocytochemistry/Immunofluorescence: MEF2C Antibody (1H5) [NBP2-00493] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEF2C.

Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human Ovary tissue using anti-MEF2C mouse monoclonal antibody.

Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEF2C antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MEF2C mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human lymph node tissue using anti-MEF2C mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MEF2C Antibody (1H5) [NBP2-00493] - Staining of paraffin-embedded Human lymphoma tissue using anti-MEF2C mouse monoclonal antibody.

Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - Analysis of Hela cells, using anti-MEF2C antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: MEF2C Antibody (1H5) [NBP2-00493] - Analysis of Jurkat cells, using anti-MEF2C antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mk
• Applications: WB, Flow, ICC/IF, IHC, IHC-P
• Clone: OTI1H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MEF2C Antibody (OTI1H5) Summary

• Immunogen: Full length recombinant protein of human MEF2C (NP_002388) produced in HEK293T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MEF2C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MEF2C Antibody (OTI1H5)

• DEL5q14.3
• MEF2C
• myocyte enhancer factor 2C
• myocyte-specific enhancer factor 2C
• polypeptide C

Background

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEF2C Antibody (NBP2-00493)(1)

Publication using NBP2-00493

• Deczkowska A, Matcovitch-Natan O, Tsitsou-Kampeli A et al. Mef2C restrains microglial inflammatory response and is lost in brain ageing in an IFN-I-dependent manner. Nat Commun. 2017 Sep 28 [PMID: 28959042] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MEF2C
• Entrez:
  • 4208(Human)
  • 17260(Mouse)
  • 499497(Rat)