HDAC7 Antibody

Western Blot: HDAC7 Antibody [NB100-2146] - Cell line lysates were separated on SDS-PAGE and probed with 1:500 Rabbit Anti-Histone Deacetylase 7 (HDAC7) (KG-17). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase ) and a chemiluminescent substrate.Lanes:1. HeLa nuclear2. NIH-3T3

Immunoprecipitation: HDAC7 Antibody [NB100-2146] - Immunoprecipitation HDAC7 was immunoprecipitated from whole cell extract of human HEK 293T cells expressing HDAC7-FLAG using Rabbit Anti-HDAC7 (KG-17) antibody (NB100-2146). The IP samples were separated on SDS-PAGE and blotted with Rabbit Anti-FLAG antibody. The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate and chemiluminescent substrate. Lane 1: 1.8 ug Antibody immunoprecipitates HDAC7 from a RIPA extract of 180 ug of 293T cells expressing HDAC7-FLAG. Lane 2: negative control (extract without antibody).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HDAC7 Antibody Summary

• Description: Novus Biologicals Rabbit HDAC7 Antibody (NB100-2146) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acids 357-373 of Human HDAC7 (RSEPLPPSATAPPPPGP) conjugated to KLH. The corresponding sequence is identical in mouse.
• Localization: Nuclear and cytoplasmic
• Specificity: Predicted molecular weight: 105 kDa. Additional bands may be detected in various extract preparations. Detection of HDAC7 is specifically inhibited with the immunizing peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunoprecipitation 1.0 - 1.5 ug
  • Western Blot 1:1000
• Application Notes: IF: Use normal rat kidney (NRK) cells. Immunoprecipitates HDAC7 from a RIPA extract of 293T cells expressing recombinant human HDAC7. WB: Use at a dilution using whole extracts of mouse NIH-3T3 cells. Optimal dilutions/concentrations should be determined by the end user.
• Theoretical MW: ~105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for HDAC7 Antibody

• DKFZP586J0917
• EC 3.5.1.98
• HD7
• HD7a
• HDAC7
• HDAC7A
• HDAC7AFLJ99588
• histone deacetylase 7
• Histone deacetylase 7ADKFZp586J0917

Background

Regulation of gene expression is mediated by several mechanisms. Among them are DNA methylation, ATP-dependent chromatin remodeling, and posttranslational modifications of histones, such as the dynamic acetylation and deacetylation of

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC7
• Entrez:
  • 51564(Human)
  • 56233(Mouse)
  • 84582(Rat)