MyoD Antibody (5.8A)

Images

 
Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.
Biological Strategies: Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected ...read more
Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by ...read more
Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/mL anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kDa in the ...read more
Biological Strategies: Western Blot: MyoD Antibody (5.8A) [NB100-56511] - MiR-106a-5p inhibited the myogenic differentiation of C2C12 myoblasts. Western blot analyzed for MyoD, MyoG, MyHC proteins 5 d post ...read more
Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)
Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
Clone
5.8A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MyoD Antibody (5.8A) Summary

Immunogen
The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
Specificity
In Rh-30, a ~45 kDa band should be observed.
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1 ug/ml
  • Knockdown Validated
  • Knockout Validated
Application Notes
There is considerable literature published using the MyoD, Clone 5.8 antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system. Knockdown and IHC-paraffin validation (PMID: 28775895). Use in FLOW reported in scientific literature (Suparamaniam U A et al). This MyoD antibody is validated for Knockout from a verified customer review.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-56511 in the following applications:

Publications
Read Publications using
NB100-56511 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

There is considerable literature published using the MyoD, Clone 5.8A antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.

Alternate Names for MyoD Antibody (5.8A)

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MyoD Antibody (NB100-56511)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, KD, WB.


Filter By Application
CyTOF-ready
(1)
Flow
(2)
ICC/IF
(5)
IHC
(1)
IHC-P
(1)
IP
(1)
KD
(1)
WB
(8)
All Applications
Filter By Species
Human
(1)
Mouse
(12)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-56511 Applications Species
Pacifici F, Della-Morte D, Piermarini F et al. Prdx6 Plays a Main Role in the Crosstalk Between Aging and Metabolic Sarcopenia Antioxidants (Basel) Apr 17 2020 [PMID: 32316601] (WB, Mouse) WB Mouse
Suparamaniam U A Stimuli-responsive Platform for Muscle Stem Cell Culture Thesis (Flow) Flow
Peng Y, Xu M, Dou M et al. MicroRNA-129-5p Inhibited C2C12 Myogenesis and Repressed Slow Fiber Gene Expression in vitro American journal of physiology. Cell physiology Apr 7 2021 [PMID: 33826407]
Fang WY, Tseng YT, Lee TY et al. Triptolide prevents lipopolysaccharide-induced skeletal muscle atrophy via inhibiting NF-kappa B/TNF-alpha and regulating protein synthesis/degradation pathway British journal of pharmacology Mar 31 2021 [PMID: 33788266]
Cai R, Zhang Q, Wang Y et al. Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis The Journal of biological chemistry Feb 3 2021 [PMID: 33548229] (WB, Mouse) WB Mouse
Takashima S, Usui S, Inoue O, et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells Scientific reports Jan 15 2021 [PMID: 33452355] (ICC/IF, Flow, Mouse) ICC/IF, Flow Mouse
Dogan SA, Cerutti R, Beninca C et al. Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy Cell Metab. Aug 9 2018 [PMID: 30122554] (ICC/IF, Mouse) ICC/IF Mouse
De Micheli AJ, Fraczek P, Soueid-Baumgarten S Single-cell analysis of the muscle stem cell hierarchy identifies heterotypic communication signals involved in skeletal muscle regeneration bioRxiv (CyTOF-ready, Mouse) CyTOF-ready Mouse
De Micheli AJ, Laurilliard EJ, Heinke CL et al. Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle Regeneration Cell Rep Mar 10 2020 [PMID: 32160558]
Li X, Zhu Y, Zhang H et al. MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIk3R1 and Modulating the PI3k/AkT Signaling. Genes (Basel). Jul 2 2018 [PMID: 30004470] (WB, Mouse) WB Mouse
Show All 20 Publications.

Reviews for MyoD Antibody (NB100-56511) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-56511:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry MyoD NB100-56511
Enlarge
5
reviewed by:
Pengpeng Bi
ICC Human 09/30/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedCell culture
SpeciesHuman
LotA-3
Western Blot MyoD NB100-56511
Enlarge
3
reviewed by:
Francesca Pacifici
WB Mouse 11/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedC2C12 mouse myoblast cell line,Differentiated C2C12 myoblast cells
SpeciesMouse
LotAB100411A-08

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MyoD Antibody (NB100-56511) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-56511AF350
Alexa Fluor 405 NB100-56511AF405
Alexa Fluor 488 NB100-56511AF488
Alexa Fluor 532 NB100-56511AF532
Alexa Fluor 594 NB100-56511AF594
Alexa Fluor 647 NB100-56511AF647
Alexa Fluor 700 NB100-56511AF700
Alexa Fluor 750 NB100-56511AF750
Biotin NB100-56511B
DyLight 350 NB100-56511UV
DyLight 405 NB100-56511V
DyLight 488 NB100-56511G
DyLight 550 NB100-56511R
DyLight 594 NB100-56511DL594
DyLight 650 NB100-56511C
DyLight 680 NB100-56511FR
DyLight 755 NB100-56511IR
FITC NB100-56511F
HRP NB100-56511H
Janelia Fluor 549 NB100-56511JF549
Janelia Fluor 646 NB100-56511JF646

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Pengpeng Bi
09/30/2020
Application: ICC
Species: Human

Francesca Pacifici
11/20/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MYOD1
Entrez
Uniprot