MyoD Antibody (5.8A)

Images

 
Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/ml anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kD in the ...read more
Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by ...read more
Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - IHC (F) using the MyoD1, Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)
Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, FeSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IP
Clone
5.8A
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

MyoD Antibody (5.8A) Summary

Immunogen
The 5.8A antibody was made against recombinant mouse MyoD protein but it also recognizes human (myf3), rat, and cat homologs. The epitope of this antibody was mapped to a region within aa 180-189 of mouse MyoD (NP_002469).
Specificity
In Rh-30, a ~45 kDa band should be observed.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOD1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1 ug/ml
Application Notes
There is considerable literature published using the MyoD, Clone 5.8 antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.
Reviewed Applications
Read 1 Review rated 3
using
NB100-56511 in the following applications:

Publications
Read Publications using
NB100-56511 in the following applications:

  • 3 publications
  • IP
    1 publication
  • WB
    5 publications

Reactivity Notes

Cat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Notes

There is considerable literature published using the MyoD, Clone 5.8A antibody. The original development publication of the MyoD antibody, Clone 5.8A showed that the antibody detected MyoD in rhabdomysosarcomas by IHC (frozen) but not in normal adult tissues (Dias, 1992) or normal fetal skeletal muscle. The 5.8A clone also detected MyoD1 in a subset of Wilms' tumors and one ectomesenchyoma, neoplasms known to contain myogenic elements. These results led to the concept in 1992 that the 5.8A clone may be useful for differentiating rhabdomyosarcomas from other soft tissue malignancies. However, as there has been a myriad of publications since Clone 5.8A was first described, users are encourage to consult the scientific literature citing Clone 5.8A to determine the suitability of the antibody for their model system.

Alternate Names for MyoD Antibody (5.8A)

  • bHLHc1
  • bHLHc1BHLHC1
  • Class C basic helix-loop-helix protein 1
  • MYF3
  • MYF3Myf-3
  • MyoD
  • MYOD1
  • MYODmyoblast determination protein 1
  • myogenic differentiation 1
  • Myogenic factor 3myf-3
  • PUM

Background

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MyoD Antibody (NB100-56511)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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ICC/IF
(3)
IP
(1)
WB
(5)
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(1)
Mouse
(6)
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Showing Publications 1 - 10 of 10.
Publications using NB100-56511 Applications Species
Dogan SA, Cerutti R, Beninca C et al. Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy Cell Metab. Aug 9 2018 [PMID: 30122554] (ICC/IF, Mouse) ICC/IF Mouse
Li X, Zhu Y, Zhang H et al. MicroRNA-106a-5p Inhibited C2C12 Myogenesis via Targeting PIk3R1 and Modulating the PI3k/AkT Signaling. Genes (Basel). Jul 2 2018 [PMID: 30004470] (WB, Mouse) WB Mouse
Jheng JR, Chen YS, Ao UI, Chan DC. The double-edged sword of endoplasmic reticulum stress in uremic sarcopenia through myogenesis perturbation. J Cachexia Sarcopenia Muscle. 2018 Jan 29 [PMID: 29380555] (Mouse) Mouse
Millington G, Elliott KH, Chang YT et al. Cilia-dependent GLI processing in neural crest cells is required for tongue development. Dev. Biol. Mar 9 2017 [PMID: 28286175] (ICC/IF, Mouse) ICC/IF Mouse
Chuang HN, Hsiao KM, Chang HY et al. The homeobox transcription factor Irxl1 negatively regulates MyoD expression and myoblast differentiation. FEBS J. 2014 May 11 [PMID: 24814716] (WB, Mouse)

Details:
C2C12 mouse myoblast cells, Fig 4D. The MyoD mAb was used at 0.25 ug/ml.
WB Mouse
Majmundar AJ, Skuli N, Mesquita RC et al. O2 regulates skeletal muscle progenitor differentiation through PI3K/AKT signaling. Molecular and Cellular Biology. 2011 Oct 17. [PMID: 22006022]
Bernardi H, Gay S, Fedon Y et al. Wnt4 activates the canonical B-catenin pathway and regulates negatively myostatin: functional implication in myogenesis. Am J Physiol Cell Physiol. 2011 May [PMID: 21248078] (WB)

Details:
MyoD (IMG-132). WB: C2C12 myoblasts transfected with Wnt4 and MRF4 satellite cells, Fig 3D & 3G.
WB
Sun Y, Ge Y, Drnevich J et al. Mammalian target of rapamycin regulates miRNA-1 and follistatin in skeletal myogenesis. J Cell Biol. 2010 Jun 28 [PMID: 20566686]
Sell H, Eckardt K, Taube A et al. Skeletal muscle insulin resistance induced by adipocyte-conditioned medium: underlying mechanisms and reversibility. Am J Physiol Endocrinol Metab. 2008 Jun [PMID: 18364460] (WB, Human)

Details:
WB (human skeletal muscle cells), Figs. 2C, 3C.
WB Human
Dias P, Parham DM, Shapiro DN et al. Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility. Cancer Res. 1992 Dec 1 [PMID: 1384962] (IP, WB, ICC/IF, Mouse)

Details:
IP (Fig 2) and WB (Fig 3): In vitro transcribed and translated mutant mouse MyoD proteins, IF/ICC (Fig4): Rh30 rhabdomyosarcoma cells, MyoD transfected/control HT1080 cells, IHC (F) (Tables 1 and 2, Fig 5A): Normal and tumor tissues. Electron microscopy (
IP, WB, ICC/IF Mouse

Review for MyoD Antibody (NB100-56511) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56511:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot MyoD NB100-56511
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reviewed by:
Francesca Pacifici
WB Mouse 11/20/2017
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Summary

ApplicationWestern Blot
Sample TestedC2C12 mouse myoblast cell line,Differentiated C2C12 myoblast cells
SpeciesMouse
LotAB100411A-08

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MyoD Antibody (NB100-56511) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56511AF405
Alexa Fluor 488 Labeled NB100-56511AF488
Alexa Fluor 647 Labeled NB100-56511AF647
Alexa Fluor 700 Labeled NB100-56511AF700
Biotin Labeled NB100-56511B
DyLight 350 Labeled NB100-56511UV
DyLight 405 Labeled NB100-56511V
DyLight 488 Labeled NB100-56511G
DyLight 550 Labeled NB100-56511R
DyLight 650 Labeled NB100-56511C
DyLight 680 Labeled NB100-56511FR
DyLight 755 Labeled NB100-56511IR
HRP Labeled NB100-56511H

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Recent Reviews

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1
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Francesca Pacifici
11/20/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MYOD1
Entrez
Uniprot