Myogenin Antibody (671037)

Images

 
Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG ...read more
Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
671037
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Myogenin Antibody (671037) Summary

Immunogen
E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173
Specificity
Detects human Myogenin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB66861 in the following applications:

Publications
Read Publication using
MAB66861 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myogenin Antibody (671037)

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Background

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56511
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
MAB4027
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
NBP1-52030
Species: Hu
Applications: WB, PEP-ELISA
AF3844
Species: Hu, Mu
Applications: IHC
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-66943
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB1675
Species: Hu, Mu, Rt, Ch
Applications: ICC
NBP2-46085
Species: Hu
Applications: WB, IHC
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
MAB4470
Species: All-Multi
Applications: WB, IHC, ICC
MAB2457
Species: Hu, Mu
Applications: WB, CyTOF-ready, ICC, ICFlow
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF6116
Species: Hu
Applications: WB, ICC
NBP2-13957
Species: Hu
Applications: WB, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
MAB66861
Species: Hu
Applications: WB, ICC/IF

Publications for Myogenin Antibody (MAB66861)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Simple Western.


Filter By Application
Simple Western
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Myogenin Antibody (MAB66861) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB66861:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Myogenin MAB66861
Enlarge
4
reviewed by:
Zachary Patinkin
Simple Western Human 02/25/2015
View

Summary

ApplicationSimple Western
Sample TestedHuman Skeletal Muscle Lysate; 3T3-L1 cell lysate
SpeciesHuman
LotCFUU0114021

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio20 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsSimple Western. Non-specific bands at higher molecular weights are consistent with results seen in traditional WB.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Myogenin Antibody (MAB66861) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Myogenin Products

Bioinformatics Tool for Myogenin Antibody (MAB66861)

Discover related pathways, diseases and genes to Myogenin Antibody (MAB66861). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Myogenin Antibody (MAB66861)

Discover more about diseases related to Myogenin Antibody (MAB66861).
 

Pathways for Myogenin Antibody (MAB66861)

View related products by pathway.

PTMs for Myogenin Antibody (MAB66861)

Learn more about PTMs related to Myogenin Antibody (MAB66861).

Blogs on Myogenin

There are no specific blogs for Myogenin, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Zachary Patinkin
02/25/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol MYOG
Uniprot