Myogenin Antibody (671037)

Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myogenin at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 671037
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Myogenin Antibody (671037) Summary

• Immunogen: E. coli-derived recombinant human Myogenin
  Ser124-Asn224
  Accession # P15173
• Specificity: Detects human Myogenin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOG
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Myogenin Antibody (671037)

• BHLHC3
• bHLHc3Myogenic factor-4; myogenin
• Class C basic helix-loop-helix protein 3
• MYF4
• myf-4
• MYF4myogenin
• MYOG
• Myogenic factor 4
• myogenin (myogenic factor 4)
• Myogenin

Background

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myogenin Antibody (MAB66861)(1)

Publication using MAB66861

• Boyle K, Patinkin Z, Shapiro A, Baker P, Dabelea D, Friedman J Mesenchymal Stem Cells from Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project. Diabetes, 0;0(0):. 0 [PMID: 26631736] (Simple Western, Human)

Review for Myogenin Antibody (MAB66861) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Zachary Patinkin Simple Western Human 02/25/2015

Summary

• Application: Simple Western
• Sample Tested: Human Skeletal Muscle Lysate; 3T3-L1 cell lysate
• Species: Human
• Lot: CFUU0114021

Blocking

• Blocking Details: Protein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

• Dilution Ratio: 20 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

• Secondary Description: Protein Simple Anti-Rabbit Secondary
• Secondary Manufacturer Cat#: 042-206
• Secondary Concentration: undiluted

Details

• Detection Notes: WES multi-image analysis

Comments

• Comments: Simple Western. Non-specific bands at higher molecular weights are consistent with results seen in traditional WB.

Bioinformatics

• Gene Symbol: MYOG
• Uniprot:
  • P15173()