Western Blot: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in NIH/3T3 lysate.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against DyLight 488 (Green) using a 40X objective. Alpha tubulin ...read more
Immunohistochemistry: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Analysis of HDAC4 in mouse pancreas.
Simple Western: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Image shows a specific band for HDAC4 in 0.5 mg/mL of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This HDAC4 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed approx. 130 kDa. In ICC/IF nuclear and cytoplasmic staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Histone Deacetylase 4/HDAC4 Antibody
Histone Deacetylase 4
histone deacetylase A
Histone deacetylase 4 (HDAC4), also known as HD4, KIAA0288, AHO3, BDMR, HDACA, and HA6116, is crucial for transcription regulation, cell cycle progression and developmental events. HDAC4 also plays a critical role in the deacetylation of lysine residues in the core histones, which results in the production of a tag for epigenetic repression. HDAC4 is a member of the class II histone deacetylase/AcuC/AphA family and has been linked to nucleosomal condensation. The HDAC4 protein is expressed ubiquitously throughout the body and can be found in both the nucleus and cytoplasm. HDAC4 gathers in the nuclei of myotubes during muscle cell differentiation, which indicates HDAC4 plays a role in muscle differentiation. HDAC4 is exported to the cytoplasm based on the interactions with a 14-3-3 chaperone protein and its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. Mutations in HDAC4 can cause brachydactyly-mental retardation syndrome (BDMR), which resembles Albright hereditary osteodystrophy. Recent research has found that HDAC4 regulates HIF-1, which influences cancer cell production and reaction to hypoxia making HDAC4 a possible protein of interest in cancer treatment (PMID: 21917920). Further research has found that HDAC4 affects the growth of cancerous cells in the prostate (PMID: 19013255). Other areas of research, involving knock-out studies, have found a link between HDAC4 and long term memory formation (PMID: 21542139).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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