Histone Deacetylase 4/HDAC4 Antibody

Images

 
Western Blot: HDAC4 Antibody [NBP2-22151] - WB analysis of HDAC4 in NIH/3T3 lysate.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - HDAC4 antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green) using a 40X objective. Alpha tubulin ...read more
Immunohistochemistry: HDAC4 Antibody [NBP2-22151] - IHC analysis of HDAC4 in mouse pancreas.
Simple Western: Histone Deacetylase 4/HDAC4 Antibody [NBP2-22151] - Simple Western lane view shows a specific band for HDAC4 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Ch, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Histone Deacetylase 4/HDAC4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human HDAC4 protein (between residues 1000-1084) [UniProt P56524]
Localization
Nucleus and cytoplasm.
Predicted Species
Zebrafish (92%), Chicken (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
Application Notes
This HDAC4 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band was observed approx. 130 kDa. In ICC/IF nuclear and cytoplasmic staining was observed in HeLa cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)
Publications
Read Publications using NBP2-22151.

Reactivity Notes

Human, mouse, rat and monkey. Immunogen sequence has 94% identity to chicken and 92% identity to Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone Deacetylase 4/HDAC4 Antibody

  • HA6116
  • HD4EC 3.5.1.98
  • HDAC4
  • HDAC-A
  • HDACABDMR
  • Histone Deacetylase 4
  • histone deacetylase A
  • KIAA0288AHO3

Background

Histone deacetylase 4 (HDAC4), also known as HD4, KIAA0288, AHO3, BDMR, HDACA, and HA6116, is crucial for transcription regulation, cell cycle progression and developmental events. HDAC4 also plays a critical role in the deacetylation of lysine residues in the core histones, which results in the production of a tag for epigenetic repression. HDAC4 is a member of the class II histone deacetylase/AcuC/AphA family and has been linked to nucleosomal condensation. The HDAC4 protein is expressed ubiquitously throughout the body and can be found in both the nucleus and cytoplasm. HDAC4 gathers in the nuclei of myotubes during muscle cell differentiation, which indicates HDAC4 plays a role in muscle differentiation. HDAC4 is exported to the cytoplasm based on the interactions with a 14-3-3 chaperone protein and its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. Mutations in HDAC4 can cause brachydactyly-mental retardation syndrome (BDMR), which resembles Albright hereditary osteodystrophy. Recent research has found that HDAC4 regulates HIF-1, which influences cancer cell production and reaction to hypoxia making HDAC4 a possible protein of interest in cancer treatment (PMID: 21917920). Further research has found that HDAC4 affects the growth of cancerous cells in the prostate (PMID: 19013255). Other areas of research, involving knock-out studies, have found a link between HDAC4 and long term memory formation (PMID: 21542139).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone Deacetylase 4/HDAC4 Antibody (NBP2-22151)(2)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Histone Deacetylase 4/HDAC4 Antibody (NBP2-22151) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HDAC4
Entrez
Uniprot