Defensin alpha 5 Antibody (8C8)

Images

 
Immunohistochemistry: Defensin alpha 5 Antibody (8C8) [NB110-60002] - Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic staining.
Flow Cytometry: Defensin alpha 5 Antibody (8C8) [NB110-60002] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Defensin alpha 5 antibody (dark blue). Isotype control shown in orange. An antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
8C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.35 mg/ml

Order Details

Defensin alpha 5 Antibody (8C8) Summary

Immunogen
Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
Localization
Secreted protein.
Specificity
This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DEFA5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dot Blot
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:1000
Application Notes
This Defensin alpha 5 antibody is useful for Immunohistochemistry paraffin embedded sections, Western blot and Immunocytochemistry/Immunofluorescence. Dot Blot and ELISA were reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30612153). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB110-60002 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
2.35 mg/ml
Purity
Protein G purified

Alternate Names for Defensin alpha 5 Antibody (8C8)

  • alpha-Defensin 5
  • DEF5
  • DEF5defensin, alpha 5, preproprotein
  • DEFA5
  • defensin 5
  • Defensin alpha 5
  • Defensin, alpha 5
  • defensin, alpha 5, Paneth cell-specific
  • defensin-5
  • HD-5
  • MGC129728

Background

Defensins are a kind of endogenous antimicrobial peptide enriched with cysteine/arginine and are widely distributed in internal organs wherein they are recognized as defenders against invasion by pathogenic organisms. Human alpha-defensins (human alpha-defensins 5, -6, and human neutrophil peptides-1, -2, -3, -4) are a class of small antimicrobial peptides with well characterized antibacterial activity against both Gram +ve as well as Gram-ve bacteria, and certain viruses such as such as HSV, HPV, adenovirus and polyoma virus. Human alpha-defensin 5 (HD5) is a 32 amino acids long protein that shares a common structural feature of alpha-defensin family characterized by three-stranded anti-parallel beta-sheets stabilized by three intra-molecular disulfide bridges. HD5 is primarily expressed in small intestinal Paneth cells or epithelial cells of urino-genital tract, and it is found in cytoplasmic vesicle/secretory vesicle or in secreted form. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues among which HD5(20-94) is the predominant intracellular form. In extracellular milieu, peptides HD5(56-94) and HD5(63-94) are found and among these two peptides, HD5 (63-94) is the most abundant form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Defensin alpha 5 Antibody (NB110-60002)(13)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: DB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
DB
(1)
ELISA
(1)
ICC/IF
(1)
IHC
(4)
IHC-Fr
(1)
IHC-P
(3)
WB
(4)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB110-60002 Applications Species
Jabari S, Schrodl F, Kaser-Eichberger A et al. Alarin in different human intestinal epithelial cell types. Histochem. Cell Biol. Jan 5 2019 [PMID: 30612153] (IHC-Fr, Human) IHC-Fr Human
Cerrillo E, Moret I, Iborra M et al. Alpha-defensins (a-Defs) in Crohn's disease: Decrease of ileal a-Def 5 via permanent methylation and increase in plasma a-Def 1-3 concentrations offering biomarker utility Clin. Exp. Immunol. 2017 Nov 28 [PMID: 29193023] (Human) Human
Múnera JO, Sundaram N, Rankin SA et al. Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling. Cell Stem Cell. 2017 Jun 20 [PMID: 28648364]
Liu TC, Naito T, Liu Z et al. LRRK2 but not ATG16L1 is associated with Paneth cell defect in Japanese Crohn's disease patients. JCI Insight. Mar 23 2017 [PMID: 28352666] (IHC-P, Human) IHC-P Human
Liu TC, Gurram B, Baldridge MT et al. Paneth cell defects in Crohn's disease patients promote dysbiosis. JCI Insight 2016 Jun 02 [PMID: 27699268]
Kim H-J, Kang U-B, Lee H et al. Profiling of differentially expressed proteins in stage IV Colorectal cancers with good and poor outcomes. Journal of Proteomics. 2011 Dec 10. [PMID: 22178445]
Jung JH, Kim HJ, Yeom J, Yoo C, Shin J, Yoo J, Kang CS, Lee C. Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. J Gastroenterol;47(1):37-48. 2012 Jan. [PMID: 22015694] (WB, Human) WB Human
Bukurova IuA, Nikitina SL, Khankin SL et al. Identification of protein markers for serum diagnosis of cancer based on microRNA expression profiling. Mol Biol (Mosk) 2011 Mar-Apr [PMID: 21634125] (WB, Human) WB Human
Wehkamp, J et al. Paneth cell antimicrobial peptides: topographical distribution and quantification in human gastrointestinal tissues. FEBS Lett;580(22):5344-50. 2006 Oct 2. [PMID: 16989824] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Wehkamp, J et al. Reduced Paneth cell alpha-defensins in ileal Crohn's disease. Proc Natl Acad Sci U S A;102(50):18129-34. 2005 Dec 13. [PMID: 16330776] (WB, IHC, Human) WB, IHC Human
Show All 13 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Defensin alpha 5.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

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Bioinformatics

Gene Symbol DEFA5
Entrez
Uniprot