GFAP Overexpression Lysate (Native)

Images

 
Western Blot: GFAP Overexpression Lysate (Adult Normal) [NBL1-11043] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GFAP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

GFAP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204548 and based on accession number NM_002055. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1, mRNA.
Gene
GFAP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
49.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for GFAP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for GFAP Overexpression Lysate (Native)

  • FLJ45472
  • GFAP astrocytes
  • GFAP
  • glial fibrillary acidic protein

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-223
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-58870
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
NB110-41404
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
NB600-717
Species: Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NB120-15200
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-45224
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-653
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB300-213
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP2-25147
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, Simple Western, ICC/IF
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NBP2-29403
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP1-22568
Species: Hu
Applications: IHC-P
NBL1-11043
Species: Hu
Applications: WB

Publications for GFAP Lysate (NBL1-11043) (0)

There are no publications for GFAP Lysate (NBL1-11043).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GFAP Lysate (NBL1-11043) (0)

There are no reviews for GFAP Lysate (NBL1-11043). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFAP Lysate (NBL1-11043). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.

Additional GFAP Products

Bioinformatics Tool for GFAP Lysate (NBL1-11043)

Discover related pathways, diseases and genes to GFAP Lysate (NBL1-11043). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GFAP Lysate (NBL1-11043)

Discover more about diseases related to GFAP Lysate (NBL1-11043).
 

Pathways for GFAP Lysate (NBL1-11043)

View related products by pathway.

PTMs for GFAP Lysate (NBL1-11043)

Learn more about PTMs related to GFAP Lysate (NBL1-11043).
 

Research Areas for GFAP Lysate (NBL1-11043)

Find related products by research area.

Blogs on GFAP.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

GFAP: Roles in Alzheimer's and Schizophrenia
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

"Whats the Hap" with GFAP?
Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi...  Read full blog post.

GFAP Antibodies are a 'No Brainer' for Neurodegenerative Research
Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Recent developments using GFAP antibodies in astrocyte biology...  Read full blog post.

Customers Who Bought This Also Bought

GFAP Antibody
NB300-141

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GFAP Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol GFAP
Entrez