Actin Gamma 1 Antibody (NH3)

Images

 
Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
NH3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Actin Gamma 1 Antibody (NH3) Summary

Immunogen
Human monocytes and U937 cell line
Specificity
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100 - 1:500
Application Notes
Actin Gamma 1 antibody validated for ICC/IF and IHC-P from verified customer reviews.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-64792 in the following applications:

Publications
Read Publications using
NB100-64792 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for Actin Gamma 1 Antibody (NH3)

  • ACT
  • ACTB
  • ACTG
  • actin, cytoplasmic 2
  • actin, gamma 1
  • cytoskeletal gamma-actin
  • deafness, autosomal dominant 20; deafness, autosomal dominant 26
  • DFNA20
  • DFNA26
  • Gamma-actin

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Actin Gamma 1 Antibody (NB100-64792)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(1)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-64792 Applications Species
Zheng Y, Li G, Luo Y et al. The RBPMSCreERT2-TdTomato Mouse for Study Retinal and Vascular Relevant Disease Available at SSRN 4417787 2023-04-13 (IHC) IHC
Chen M, Menon MC, Wang W et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy Nature communications 2023-07-18 [PMID: 37463911] (ICC/IF, Mouse) ICC/IF Mouse
Hsieh Y, Hsu W, Yang S et al. Potential Antimetastatic Effect of Timosaponin AIII against Human Osteosarcoma Cells through Regulating the Integrin/FAK/Cofilin Axis Pharmaceuticals 2021-03-14 [PMID: 33799345] (IF/IHC, Mouse) IF/IHC Mouse
Wendt TS, Li YJ, Gonzales RJ Ozanimod, an S1PR1 ligand, attenuates hypoxia plus glucose deprivation induced autophagic flux and phenotypic switching in human brain VSM cells American journal of physiology. Cell physiology 2021-03-31 [PMID: 33788630]
Hall G, Lane BM, Khan K et al. The Human FSGS-Causing ANLN R431C Mutation Induces Dysregulated PI3K/AKT/mTOR/Rac1 Signaling in Podocytes. J. Am. Soc. Nephrol. 2018-08-01 [PMID: 30002222]
Liu R, Lauridsen HM, Amezquita RA et al. IL-17 Promotes Neutrophil-Mediated Immunity by Activating Microvascular Pericytes and Not Endothelium. J. Immunol. 2016-08-17 [PMID: 27534549] (ICC/IF, Human) ICC/IF Human
Goncalves NP, Martins D, Saraiva MJ. Overexpression of Protocadherin-10 in Transthyretin-Related Familial Amyloidotic Polyneuropathy. Am. J. Pathol. 2016-07-01 [PMID: 27338109]
Cheng WY, Chiao MT, Liang YJ et al. Luteolin inhibits migration of human glioblastoma U-87 MG and T98G cells through downregulation of Cdc42 expression and PI3K/AKT activity. Mol Biol Rep 2013-05-16 [PMID: 23677714] (WB, Human) WB Human

Reviews for Actin Gamma 1 Antibody (NB100-64792) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-64792:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Actin Gamma 1 NB100-64792
Enlarge
3
reviewed by:
Verified Customer
IHC-P Human 10/30/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedinner ear
SpeciesHuman
Lot148238
  5
reviewed by:
Chan-Yen Kuo
IF Human 07/05/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Gamma 1 Antibody (NB100-64792) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on Actin Gamma 1

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Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Verified Customer
10/30/2019
Application: IHC-P
Species: Human

Chan-Yen Kuo
07/05/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACTG1
Entrez
Uniprot