Actin Gamma 1 Antibody (NH3)

Images

 
Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.
Immunohistochemistry-Paraffin: Actin Gamma 1 Antibody (NH3) [NB100-64792] - Human inner ear tissue section stained with Actin Gamma 1 antibody at 1:100 overnight at 4C and detected with Alexa Fluor 488 Donkey anti-mouse ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
NH3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Actin Gamma 1 Antibody (NH3) Summary

Immunogen
Human monocytes and U937 cell line
Specificity
Recognizes human Filamentous actin (F-actin). The binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
ACTG1
Purity
IgM purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:500
  • ELISA 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin
Application Notes
Actin Gamma 1 antibody validated for ICC/IF and IHC-P from verified customer reviews.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-64792 in the following applications:

Publications
Read Publications using
NB100-64792 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgM purified

Alternate Names for Actin Gamma 1 Antibody (NH3)

  • ACT
  • ACTB
  • ACTG
  • actin, cytoplasmic 2
  • actin, gamma 1
  • cytoskeletal gamma-actin
  • deafness, autosomal dominant 20; deafness, autosomal dominant 26
  • DFNA20
  • DFNA26
  • Gamma-actin

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single Cell Western
NBP2-15698
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-58268
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-52559
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-76966
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
7270-IL/CF
Species: Hu
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IF
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, Gp, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze, Dr(-)
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ChIP, KD, KO
NBP2-13075
Species: Hu, Mu, Rt, Pm
Applications: WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NBP2-59208
Species: Hu, Mu
Applications: WB, ChIP, CHIP-SEQ, KD

Publications for Actin Gamma 1 Antibody (NB100-64792)(4)

Reviews for Actin Gamma 1 Antibody (NB100-64792) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-64792:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Actin Gamma 1 NB100-64792
Enlarge
reviewed by:
Anonymous
IHC-P Human 10/30/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedinner ear
SpeciesHuman
Lot148238
Immunofluorescence Actin Gamma 1 NB100-64792
Enlarge
reviewed by:
Chan-Yen Kuo
IF Human 07/05/2013
View

Summary

ApplicationImmunofluorescence
SpeciesHuman
Pub Med IDdoi: 10.1016/j.virusres.2013.03.017. Epub 2013 Apr 13
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Actin Gamma 1 Antibody (NB100-64792) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Actin Gamma 1 Products

Bioinformatics Tool for Actin Gamma 1 Antibody (NB100-64792)

Discover related pathways, diseases and genes to Actin Gamma 1 Antibody (NB100-64792). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Actin Gamma 1 Antibody (NB100-64792)

Discover more about diseases related to Actin Gamma 1 Antibody (NB100-64792).
 

Pathways for Actin Gamma 1 Antibody (NB100-64792)

View related products by pathway.

PTMs for Actin Gamma 1 Antibody (NB100-64792)

Learn more about PTMs related to Actin Gamma 1 Antibody (NB100-64792).

Blogs on Actin Gamma 1

There are no specific blogs for Actin Gamma 1, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
1
4
0
3
1
2
0
1
0

Anonymous
10/30/2019
Application: IHC-P
Species: Human

Chan-Yen Kuo
07/05/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol ACTG1
Entrez
Uniprot
COVID-19 update