FoxP3 Antibody (3G3) Summary
Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).
Protein G purified
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- Western Blot 2-5 ug/ml
- Flow Cytometry 100 ng/ 10^6 cells in 50 ul
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Cross reacts with Human and Mouse.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS containing 0.2% gelatin
0.05% Sodium Azide
Protein G purified
Alternate Names for FoxP3 Antibody (3G3)
- forkhead box P3
- Forkhead Box Protein P3
- foxp3 flow cytometry
- foxp3 flow
- immune dysregulation, polyendocrinopathy, enteropathy, X-linked
- Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
- XPIDpolyendocrinopathy, enteropathy, X-linked
One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Publications for FoxP3 Antibody (NB100-56582)(2)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for FoxP3 Antibody (NB100-56582). (Showing 1 - 4 of 4 FAQs).
Does FoxP3 antibodies comes in lyophilized form?
- FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
What the theoretical molecular weight for FoxP3 antibodies?
- The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?
- Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.
What is the immunogen sequence of this FoxP3 antibody?
- The full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.
Positive Control Lysate(s)
Other Available Formats
Additional Array Products
Bioinformatics Tool for FoxP3 Antibody (NB100-56582)
Discover related pathways, diseases and genes to FoxP3 Antibody (NB100-56582). Need help?
Read the Bioinformatics Tool Guide
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Blogs on FoxP3.
Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde... Read full blog post.
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively,... Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of... Read full blog post.
FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post.