FoxP3 Antibody (3G3)

Images

 
Western Blot: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Azide Free version of NB100-56582. Detection of 1) full-length transfected and 2) mock transfected 293 cell lysate at 2 ug/ml.
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control ...read more
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and ...read more
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Azide Free version of NB100-56582. Staining of 2x10^6 mouse lymph node cells using 0.1 ug of PE conjugated antibody.
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Azide Free version of NB100-56582. Staining of human PBMCs stimulated with anti-hCD3 and rhIL-2 for 48 hours using isotype control (left) and ...read more
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the Allophycocyanin conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype ...read more
Flow Cytometry: FoxP3 Antibody (3G3) [NB100-56582] - Analysis using the FITC conjugate of NB100-56582. Staining of FOXP3 in mouse splenocytes stimulated with anti-mCD3 and rmIL-2 for 48 hours using isotype control ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow
Clone
3G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

FoxP3 Antibody (3G3) Summary

Immunogen
Full-length His-tagged murine FOXP3 was used as the immunogen. The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein (NP_001107849).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXP3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-5 ug/ml
  • Flow Cytometry 100 ng/ 10^6 cells in 50 ul
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
FoxP3 Lysate (NBL1-10817)
Lymph Node Lysate (NB820-59242)
Publications
Read Publications using NB100-56582.

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for FoxP3 Antibody (3G3)

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 flow cytometry
  • foxp3 flow
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

One of the many immunotolerance mechanisms that the immune system has developed to distinguish between self and non-self antigens is regulatory T cells or Tregs. Several elegant experiments using transgenic mice and retrovirus-mediated over expression studies, have led to the identification of FoxP3, a transcription factor, as a specific molecular marker essential for the development and function of Tregs. The primary evidence regarding the involvement of FoxP3 in the development of Tregs was provided in patients suffering from IPEX, a rare and fatal human autoimmune disease. The emergence of Tregs and the role of FoxP3 as a critical player in the negative control of various normal and pathological immune responses holds great promise for the development of novel therapies for autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (NB100-56582)(2)


Showing Publications 1 - 2 of 2.
Publications using NB100-56582 Applications Species
Gavin MA, Torgerson TR, Houston E et al. Single-cell analysis of normal and FOXP3-mutant human T cells: FOXP3 expression without regulatory T cell development. Proc Natl Acad Sci U S A. 2006 Apr 25 [PMID: 16617117]
Wells JC, Smith Ann Davenport, V Morse R. The influence of regulatory T cells and mobilisation regimens on graft versus malignancy, graft versus host disease and relapse in haematopoietic progenitor cell transplantation (DBMS, University of the West of England).. Thesis. 2015 May 06

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FoxP3 Antibody (NB100-56582). (Showing 1 - 4 of 4 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. Since the protocol to isolate cells from mice seems to be quite long I would like to know if you have experience in staining with Foxp3 and Ror gamma and leaving cells overnight? Would you suggest leaving cells overnight in Fix/Perm or better after having completed the staining? And in which buffer would you leave them then?
    • Where as FOXP3 and ROR gamma staining can be stopped after the fixation step, we suggest that researchers strictly follow the protocol without variation (even if it takes a long time), when trying for the 1st time. After you establish this for your system, then you can work on optimizing a varied protocol according to your needs and convenience. In this case, after the fix/perm step (Step 13 of NBP2-266911) wash the cells in 1X Staining buffer (2 times) and suspend in Staining buffer (500 ul) overnight. Then, in the morning proceed to Step 14 and continue the protocol.
  4. What is the immunogen sequence of this FoxP3 antibody?
    • The full-length His-tagged murine FOXP3 was used as the immunogen.  The precise epitope is not known, but it has been mapped to the N-terminal portion of the protein.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56582AF405
Alexa Fluor 488 Labeled NB100-56582AF488
Alexa Fluor 647 Labeled NB100-56582AF647
Alexa Fluor 700 Labeled NB100-56582AF700
Allophycocyanin Labeled NBP2-26671
Biotin Labeled NB100-56582B
DyLight 350 Labeled NB100-56582UV
DyLight 405 Labeled NB100-56582V
DyLight 488 Labeled NB100-56582G
DyLight 550 Labeled NB100-56582R
DyLight 650 Labeled NB100-56582C
DyLight 680 Labeled NB100-56582FR
DyLight 755 Labeled NB100-56582IR
FITC Labeled NBP2-26668
PE Labeled NBP2-33297PE
PerCP Labeled NB100-56582PCP

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Bioinformatics Tool for FoxP3 Antibody (NB100-56582)

Discover related pathways, diseases and genes to FoxP3 Antibody (NB100-56582). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for FoxP3 Antibody (NB100-56582)

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Blogs on FoxP3.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively,...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot