Human Lymph Node Whole Tissue Lysate (Adult Whole Normal)

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Western Blot: Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) [NB820-59242] - Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Lymph Node Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Lymph Node

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES, pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Detergent and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Lymph Node

Type
Tissue
Life Stage
Adult
Tissue
Blood Lymph
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

WB Video Protocol

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