eIF4A2 Antibody (PCRP-EIF4A2-2B5)

Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4A2 Mouse Monoclonal Antibody (PCRP-eIF4A2-2B5).

• Reactivity: Hu
• Applications: ELISA, MA
• Clone: PCRP-EIF4A2-2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eIF4A2 Antibody (PCRP-EIF4A2-2B5) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08883)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human eIF4A2 protein (Uniprot: Q14240)
• Localization: Nucleus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF4A2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Protein Array
• Application Notes: ELISA: For coating, order Ab without BSA.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for eIF4A2 Antibody (PCRP-EIF4A2-2B5)

• ATP-dependent RNA helicase eIF4A-2
• BM-010
• DDX2Beukaryotic translation initiation factor 4A, isoform 2
• EC 3.6.1
• EC 3.6.4.13
• EIF4A
• eIF-4A-II
• eIF4A-II
• EIF4F
• eukaryotic initiation factor 4A-II
• eukaryotic translation initiation factor 4A
• eukaryotic translation initiation factor 4A2

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4A2
• Uniprot:
  • Q14240(Human)