eIF4B Antibody

Images

 
Independent Antibodies: Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

eIF4B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 561 and 611 of human eukaryotic translation initiation factor 4B using the numbering given in entry NP_001408.2 (GeneID 1975).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Positive Control
eIF4B Lysate (NBP2-66060)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-93308 in the following applications:

Publications
Read Publication using
NB100-93308 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4B Antibody

  • Eif4a2
  • eIF4B
  • EIF-4B
  • eukaryotic translation initiation factor 4B
  • PRO1843

Background

Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-93308
Species: Hu
Applications: WB, IP

Publications for eIF4B Antibody (NB100-93308)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
All Species

Reviews for eIF4B Antibody (NB100-93308) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-93308:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot eIF4B NB100-93308
Enlarge
reviewed by:
Wei-Ting Lu
WB Human 06/22/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lota1
Commentsspecific band observed ta 70 kD as predicted in swissprot and verified with siRNA. non-specific band at approx 120kD

Blocking

Blocking Details5% milk in TBST

Primary Anitbody

Dilution Ratio1/2000

Secondary Antibody

Secondary DescriptionLi-Cor Goat anti-Rabbit IRdye800
Secondary Concentration1/15000

Details

Detection NotesLi-Cor Odyssey scanner

Comments

Commentsspecific band observed ta 70 kD as predicted in swissprot and verified with siRNA. non-specific band at approx 120kD
Western Blot eIF4B NB100-93308
Enlarge
reviewed by:
Wei-Ting Lu
WB Human 05/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 15ug/well
SpeciesHuman
LotA1

Blocking

Blocking DetailsBlocking Buffer: 5% milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:2000, Incubation Dilution Buffer: 3% milk in TBST, Incubation Time: overnight, Incubation Temp: 4 C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit LI-COR IR800 antibody, Secondary Ab Dilution Ratio: 1:15000

Details

Detection NotesDetection Method: LI-COR odyssey scanner, Exposure Time: intensity 7.5, Specific Bands: 80kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eIF4B Antibody (NB100-93308) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Wei-Ting Lu
06/22/2012
Application: WB
Species: Human

Wei-Ting Lu
05/27/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol EIF4B
Entrez
Uniprot