eIF4B Antibody

Western Blot: eIF4B Antibody [NB100-93308] - Detection of Human eIF4B on HeLa whole cell lysate using NB100-93308. eIF4B was also immunoprecipitated by rabbit anti-eIF4B antibody NB100-93307.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eIF4B Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 561 and 611 of human eukaryotic translation initiation factor 4B using the numbering given in entry NP_001408.2 (GeneID 1975).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4B Antibody

• Eif4a2
• eIF4B
• EIF-4B
• eukaryotic translation initiation factor 4B
• PRO1843

Background

Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4B Antibody (NB100-93308)(2)

Publications using NB100-93308

• Galan A, Lozano G, Pineiro D, Martinez-Salas E. G3BP1 interacts directly with the FMDV IRES and negatively regulates translation FEBS J. 2017-07-29 [PMID: 28755480] (WB, Human)
• Meijer HA, Kong YW, Lu W et al. Translational repression and eIF4A2 activity are critical for miRNA mediated gene regulation. Science 2013-04-05 [PMID: 23559250] (WB, Human)

Reviews for eIF4B Antibody (NB100-93308) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/22/2012

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: a1

reviewed by: Verified Customer WB Human 05/27/2011

Summary

• Application: Western Blot
• Sample Tested: HeLa cell lysate, Sample Amount: 15ug/well
• Species: Human
• Lot: A1

Bioinformatics

• Gene Symbol: EIF4B
• Entrez:
  • 1975(Human)
• Uniprot:
  • P23588()