p70 S6 Kinase/S6K [p Thr389/412] Antibody

Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Abeta facilitates HIF1alpha synthesis and autophagy inhibition via mTOR activation. Cells were incubated with rapamycin (10 nM) for 30 min prior to Abeta treatment for 24 h. Phosphorylation of 4EBP1 (Thr 37/46) and 4EBP1, phosphorylation of p70S6K1 (Thr 389), HIF1alpha and beta-actin were analyzed by western blot. n = 6. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fnmol.2017.00229/full) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.

Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections

Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of phospho-p70 S6 Kinase in human breast cancer cell lines (MDA-MB-231 and MDA-MB-435) using p70 S6 Kinase/S6K [p Thr390, p Thr412] Antibody. Image from verified customer review.

Western Blot: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of p70 S6 Kinase phosphorylation expression in Insulin treatedJurkat whole cell lysates. The lane on the left is treated with the antigen-specific peptide.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p70 S6 Kinase/S6K [p Thr389/412] Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab2571)
• Immunogen: Synthetic phospho-peptide surrounding amino acids Thr389/412 of human p70 S6 Kinase/S6K
• Modification: p Thr389/412
• Specificity: p70 S6 Kinase/S6K [p Thr389/412] Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Threonine 389/412
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KB1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Application Notes: Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID:32339950).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150 mM EDTA, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for p70 S6 Kinase/S6K [p Thr389/412] Antibody

• EC 2.7.11
• EC 2.7.11.1
• p70 ribosomal S6 kinase alpha
• p70 S6 kinase alpha
• p70 S6 Kinase
• p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
• p70 S6 kinase, alpha 2
• p70 S6KA
• p70 S6K-alpha
• p70(S6K)-alpha
• p70-alpha
• p70-S6K
• P70S6K1
• PS6K
• ribosomal protein S6 kinase beta-1
• Ribosomal protein S6 kinase I
• ribosomal protein S6 kinase, 70kD, polypeptide 1
• ribosomal protein S6 kinase, 70kDa, polypeptide 1
• RPS6KB1
• S6K
• S6K1
• S6K1p70-S6K 1
• S6K-beta-1
• serine/threonine kinase 14 alpha
• Serine/threonine-protein kinase 14A
• STK14A

Background

p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 prime oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for p70 S6 Kinase/S6K Antibody (NB600-1049)(6)

Publications using NB600-1049

• Hashikawa-Hobara N, Otsuka A, Okujima C, Hashikawa N CGRP overexpression does not alter depression-like behavior in mice PeerJ 2021-07-02 [PMID: 34249519] (WB)
• Chen S, Lu XT, He TT et al. Betaine Delayed Muscle Loss by Attenuating Samtor Complex Inhibition for mTORC1 Signaling Via Increasing SAM Level Molecular nutrition & food research 2021-06-01 [PMID: 34061446]
• Gao K, Niu J, Dang X Wnt-3a improves functional recovery through autophagy activation via inhibiting the mTOR signaling pathway after spinal cord injury Neurosci. Lett. 2020-08-17 [PMID: 32818590] (WB, Rat)
• Zhang Q, Wei D, Tan M et al. Transgenic expression of Sag/Rbx2 E3 causes early stage tumor promotion, late stage cytogenesis and acinar loss in the Kras-PDAC model Neoplasia 2020-04-24 [PMID: 32339950] (IHC-P, IF/IHC, Mouse)
• Lee HJ, Jung YH, Choi GE et al. O-cyclic phytosphingosine-1-phosphate stimulates HIF1 alpha-dependent glycolytic reprogramming to enhance the therapeutic potential of mesenchymal stem cells Cell Death Dis 2019-08-05 [PMID: 31383843] (WB)
• Lee KH, Lee S, Lee HJ et al. Amyloid B1-42 (AB1-42) Induces the CDK2-Mediated Phosphorylation of Tau through the Activation of the mTORC1 Signaling Pathway While Promoting Neuronal Cell Death Front. Mol. Neurosci. 2017-07-24 [PMID: 28790888] (WB, Human)

Review for p70 S6 Kinase/S6K Antibody (NB600-1049) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Haiquan Lu WB Human 08/16/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: RPS6KB1
• Uniprot:
  • Q9UBS0()
  • NP_003152(Human)
  • P23443(Human)
  • NP_001107806.1(Mouse)
  • Q8BSK8(Mouse)
  • NP_114191.1(Rat)
  • P67999(Rat)