p70 S6 Kinase/S6K [p Ser389] Antibody

Images

 
Western Blot: p70 S6 Kinase/S6K [p Thr389] Antibody [NB600-1049] - Analysis of p70 S6 Kinase phosphorylation expression in Insulin treatedJurkat whole cell lysates. The lane on the left is treated with the ...read more
Immunocytochemistry/ Immunofluorescence: p70 S6 Kinase/S6K [p Ser389] Antibody [NB600-1049] - p70 S6 Kinase/S6K [p Thr389] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.
Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389] Antibody [NB600-1049] - Human liver cancer tissue sections
Western Blot: p70 S6 Kinase/S6K [p Ser389] Antibody [NB600-1049] - Analysis of phospho-p70 S6 Kinase in human breast cancer cell lines (MDA-MB-231 and MDA-MB-435) using anti-p70 S6 Kinase [p Thr389] antibody. Image from ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

p70 S6 Kinase/S6K [p Ser389] Antibody Summary

Immunogen
Synthetic phospho-peptide surrounding amino acid Ser389 of human p70 S6 kinase
Modification
p Ser389
Specificity
This detects p70 S6 Kinase only when phosphorylated at Thr389.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6KB1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:50 - 1:200
Reviewed Applications
Read 1 Review rated 5
using
NB600-1049 in the following applications:

Publications
Read Publication using
NB600-1049 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 150 mM EDTA, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for p70 S6 Kinase/S6K [p Ser389] Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

p70 S6 Kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression. P70 S6 Kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 prime oligopyrimidine tract mRNAs. A second isoform, p85 S6 Kinase, is derived from the same gene and is identical to p70 S6 Kinase except for 23 extra residues at the N-terminus that encode a nuclear localizing signal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for p70 S6 Kinase/S6K Antibody (NB600-1049)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for p70 S6 Kinase/S6K Antibody (NB600-1049) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB600-1049:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot p70 S6 Kinase/S6K NB600-1049
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reviewed by:
Anonymous
WB Human 08/16/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

View specific protocols for p70 S6 Kinase/S6K Antibody (NB600-1049): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p70 S6 Kinase/S6K Antibody (NB600-1049) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for p70 S6 Kinase/S6K Antibody (NB600-1049)

Discover related pathways, diseases and genes to p70 S6 Kinase/S6K Antibody (NB600-1049). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on p70 S6 Kinase/S6K.

S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.

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Recent Reviews

5
5
1
4
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3
0
2
0
1
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Anonymous
08/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RPS6KB1
Entrez
Uniprot