4EBP1 Antibody


Genetic Strategies: Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, KO
1.0 mg/ml
Validated by:

Genetic Strategies


4EBP1 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1:5000-1:20000
  • Immunohistochemistry-Paraffin 1:5000-1:20000
  • Knockout Validated
  • Western Blot 1:1000-1:6000
Application Notes
For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.
4EBP1 Overexpression Lysate
Read Publications using NB200-157.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1


eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 4EBP1 Antibody (NB200-157)(45)

We have publications tested in 1 confirmed species: Human.

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Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB200-157 Applications Species
Jozefczuk J, Kashofer K, Ummanni R et al. A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells Front Physiol 2012-01-01 [PMID: 22969728] (Human) Human
Kobayashi M, Arias C, Garabedian A et al. Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication. J Virol 2012-10-01 [PMID: 22837200]
Lang SM, Kazi AA, Hong-Brown L et al. Delayed recovery of skeletal muscle mass following hindlimb immobilization in mTOR heterozygous mice. PLoS One 2012-01-01 [PMID: 22745686]
Winter JN, Fox TE, Kester M et al. Phosphatidic acid mediates activation of mTORC1 through the ERK signaling pathway. Am J Physiol Cell Physiol 2010-08-01 [PMID: 20427710]
Konsavage W, Zhang L, Vary T et al. Hyperoxia inhibits protein synthesis and increases eIF2alpha phosphorylation in the newborn rat lung. Am J Physiol Lung Cell Mol Physiol 2010-05-01 [PMID: 20139179]
Wilson FA, Suryawan A, Orellana RA et al. Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation. J Nutr 2009-10-01 [PMID: 19692527]
Baum JI, Kimball SR, Jefferson LS et al. Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver. Am J Physiol Endocrinol Metab 2009-08-01 [PMID: 19509187]
Tyler WA, Gangoli N, Gokina P et al. Activation of the mammalian target of rapamycin (mTOR) is essential for oligodendrocyte differentiation. J Neurosci 2009-05-01 [PMID: 19439614]
Norton LE, Layman DK, Bunpo P et al. The leucine content of a complete meal directs peak activation but not duration of skeletal muscle protein synthesis and mammalian target of rapamycin signaling in rats. J Nutr 2009-06-01 [PMID: 19403715]
Hwee DT, Bodine SC. Age-related deficit in load-induced skeletal muscle growth. J Gerontol A Biol Sci Med Sci 2009-06-01 [PMID: 19351696]
Show All 45 Publications.

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Control Lysate(s)

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Gene Symbol EIF4EBP1