4EBP1 Antibody

Images

 
Genetic Strategies: Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

4EBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
Localization
Cytoplasmic
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4EBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:6000
  • Immunohistochemistry 1:5000-1:20000
  • Immunohistochemistry-Paraffin 1:5000-1:20000
  • Knockout Validated
Application Notes
For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.
Control
4EBP1 Lysate (NBL1-10206)
4EBP1 Lysate (NBP2-64630)
Publications
Read Publications using NB200-157.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

  • 4EBP1
  • 4E-BP1
  • eIF4E-binding protein 1
  • EIF4EBP1
  • eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
  • eukaryotic translation initiation factor 4E-binding protein 1
  • PHAS-I
  • PHAS-IMGC4316
  • Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation.. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for 4EBP1 Antibody (NB200-157)(45)

We have publications tested in 1 confirmed species: Human.


Filter By Application
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Filter By Species
Human
(1)
All Species
Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using NB200-157 Applications Species
Jozefczuk J, Kashofer K, Ummanni R et al. A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells Front Physiol 2012 [PMID: 22969728] (Human) Human
Kobayashi M, Arias C, Garabedian A et al. Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication. J Virol 2012 Oct [PMID: 22837200]
Lang SM, Kazi AA, Hong-Brown L et al. Delayed recovery of skeletal muscle mass following hindlimb immobilization in mTOR heterozygous mice. PLoS One 2012 [PMID: 22745686]
Winter JN, Fox TE, Kester M et al. Phosphatidic acid mediates activation of mTORC1 through the ERK signaling pathway. Am J Physiol Cell Physiol 2010 Aug [PMID: 20427710]
Konsavage W, Zhang L, Vary T et al. Hyperoxia inhibits protein synthesis and increases eIF2alpha phosphorylation in the newborn rat lung. Am J Physiol Lung Cell Mol Physiol 2010 May [PMID: 20139179]
Wilson FA, Suryawan A, Orellana RA et al. Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation. J Nutr 2009 Oct [PMID: 19692527]
Baum JI, Kimball SR, Jefferson LS et al. Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver. Am J Physiol Endocrinol Metab 2009 Aug [PMID: 19509187]
Tyler WA, Gangoli N, Gokina P et al. Activation of the mammalian target of rapamycin (mTOR) is essential for oligodendrocyte differentiation. J Neurosci 2009 May [PMID: 19439614]
Norton LE, Layman DK, Bunpo P et al. The leucine content of a complete meal directs peak activation but not duration of skeletal muscle protein synthesis and mammalian target of rapamycin signaling in rats. J Nutr 2009 Jun [PMID: 19403715]
Hwee DT, Bodine SC. Age-related deficit in load-induced skeletal muscle growth. J Gerontol A Biol Sci Med Sci 2009 Jun [PMID: 19351696]
Show All 45 Publications.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF4EBP1
Entrez
Uniprot