4EBP1 Antibody

Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human 4EBP1 Polyclonal Antibody (Catalog # NB200-157) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for 4EBP1 at approximately 17-20 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.

Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

4EBP1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
• Localization: Cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4EBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:5000-1:20000
  • Immunohistochemistry-Paraffin 1:5000-1:20000
  • Knockout Validated
  • Western Blot 1:1000-1:6000
• Application Notes: For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.
• Control:

  4EBP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for 4EBP1 Antibody

• 4EBP1
• 4E-BP1
• eIF4E-binding protein 1
• EIF4EBP1
• eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
• eukaryotic translation initiation factor 4E-binding protein 1
• PHAS-I
• PHAS-IMGC4316
• Phosphorylated heat- and acid-stable protein regulated by insulin 1

Background

eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for 4EBP1 Antibody (NB200-157)(45)

Publications using NB200-157

• Jozefczuk J, Kashofer K, Ummanni R et al. A Systems Biology Approach to Deciphering the Etiology of Steatosis Employing Patient-Derived Dermal Fibroblasts and iPS Cells Front Physiol 2012-01-01 [PMID: 22969728] (Human)
• Kobayashi M, Arias C, Garabedian A et al. Site-specific cleavage of the host poly(A) binding protein by the encephalomyocarditis virus 3C proteinase stimulates viral replication. J Virol 2012-10-01 [PMID: 22837200]
• Lang SM, Kazi AA, Hong-Brown L et al. Delayed recovery of skeletal muscle mass following hindlimb immobilization in mTOR heterozygous mice. PLoS One 2012-01-01 [PMID: 22745686]
• Winter JN, Fox TE, Kester M et al. Phosphatidic acid mediates activation of mTORC1 through the ERK signaling pathway. Am J Physiol Cell Physiol 2010-08-01 [PMID: 20427710]
• Konsavage W, Zhang L, Vary T et al. Hyperoxia inhibits protein synthesis and increases eIF2alpha phosphorylation in the newborn rat lung. Am J Physiol Lung Cell Mol Physiol 2010-05-01 [PMID: 20139179]
• Wilson FA, Suryawan A, Orellana RA et al. Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation. J Nutr 2009-10-01 [PMID: 19692527]
• Baum JI, Kimball SR, Jefferson LS et al. Glucagon acts in a dominant manner to repress insulin-induced mammalian target of rapamycin complex 1 signaling in perfused rat liver. Am J Physiol Endocrinol Metab 2009-08-01 [PMID: 19509187]
• Tyler WA, Gangoli N, Gokina P et al. Activation of the mammalian target of rapamycin (mTOR) is essential for oligodendrocyte differentiation. J Neurosci 2009-05-01 [PMID: 19439614]
• Norton LE, Layman DK, Bunpo P et al. The leucine content of a complete meal directs peak activation but not duration of skeletal muscle protein synthesis and mammalian target of rapamycin signaling in rats. J Nutr 2009-06-01 [PMID: 19403715]
• Hwee DT, Bodine SC. Age-related deficit in load-induced skeletal muscle growth. J Gerontol A Biol Sci Med Sci 2009-06-01 [PMID: 19351696]
• Pruznak AM, Kazi AA, Frost RA et al. Activation of AMP-activated protein kinase by 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside prevents leucine-stimulated protein synthesis in rat skeletal muscle. J Nutr 2008-10-01 [PMID: 18806097]
• Pruznak AM, Hong-Brown L, Lantry R et al. Skeletal and cardiac myopathy in HIV-1 transgenic rats. Am J Physiol Endocrinol Metab 2008-10-01 [PMID: 18713959]
• Wilson FA, Suryawan A, Orellana RA et al. Fed levels of amino acids are required for the somatotropin-induced increase in muscle protein synthesis. Am J Physiol Endocrinol Metab 2008-10-01 [PMID: 18682537]
• Wilson FA, Orellana RA, Suryawan A et al. Stimulation of muscle protein synthesis by somatotropin in pigs is independent of the somatotropin-induced increase in circulating insulin. Am J Physiol Endocrinol Metab 2008-07-01 [PMID: 18460595]
• Walsh D, Arias C, Perez C et al. Eukaryotic translation initiation factor 4F architectural alterations accompany translation initiation factor redistribution in poxvirus-infected cells. Mol Cell Biol 2008-04-01 [PMID: 18250159]
• Kimball SR, Do AN, Kutzler L et al. Rapid turnover of the mTOR complex 1 (mTORC1) repressor REDD1 and activation of mTORC1 signaling following inhibition of protein synthesis. J Biol Chem 2008-02-01 [PMID: 18070882]
• Suryawan A, Orellana RA, Nguyen HV et al. Activation by insulin and amino acids of signaling components leading to translation initiation in skeletal muscle of neonatal pigs is developmentally regulated. Am J Physiol Endocrinol Metab 2007-12-01 [PMID: 17878222]
• Orellana RA, Jeyapalan A, Escobar J et al. Amino acids augment muscle protein synthesis in neonatal pigs during acute endotoxemia by stimulating mTOR-dependent translation initiation. Am J Physiol Endocrinol Metab 2007-11-01 [PMID: 17848637]
• Lamouille S, Derynck R. Cell size and invasion in TGF-beta-induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway. J Cell Biol 2007-07-01 [PMID: 17646396]
• Jeyapalan AS, Orellana RA, Suryawan A et al. Glucose stimulates protein synthesis in skeletal muscle of neonatal pigs through an AMPK- and mTOR-independent process. Am J Physiol Endocrinol Metab 2007-08-01 [PMID: 17551002]
• Shaheen HH, Horetsky RL, Kimball SR et al. Retrograde nuclear accumulation of cytoplasmic tRNA in rat hepatoma cells in response to amino acid deprivation. Proc Natl Acad Sci U S A 2007-05-01 [PMID: 17502605]
• Lang CH, Frost RA, Vary TC et al. Skeletal muscle protein synthesis and degradation exhibit sexual dimorphism after chronic alcohol consumption but not acute intoxication. Am J Physiol Endocrinol Metab 2007-06-01 [PMID: 17264221]
• Anthony TG, McDaniel BJ, Knoll P et al. Feeding meals containing soy or whey protein after exercise stimulates protein synthesis and translation initiation in the skeletal muscle of male rats. J Nutr 2007-02-01 [PMID: 17237311]
• Orellana RA, Kimball SR, Suryawan A et al. Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation. Am J Physiol Endocrinol Metab 2007-02-01 [PMID: 17047163]
• Vary TC, Kimball SR, Sumner A et al. Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption. Am J Physiol Regul Integr Comp Physiol 2007-02-01 [PMID: 16946086]
• Vary TC, Lynch CJ. Meal feeding stimulates phosphorylation of multiple effector proteins regulating protein synthetic processes in rat hearts. J Nutr 2006-09-01 [PMID: 16920842]
• Arvisais EW, Romanelli A, Hou X et al. AKT-independent phosphorylation of TSC2 and activation of mTOR and ribosomal protein S6 kinase signaling by prostaglandin F2alpha. J Biol Chem 2006-09-01 [PMID: 16816403]
• Williamson DL, Bolster DR, Kimball SR et al. Time course changes in signaling pathways and protein synthesis in C2C12 myotubes following AMPK activation by AICAR. Am J Physiol Endocrinol Metab 2006-07-01 [PMID: 16760336]
• Caso G, Garlick PJ, Ballou LM et al. The increase in human muscle protein synthesis induced by food intake is similar when assessed with the constant infusion and flooding techniques. J Nutr 2006-06-01 [PMID: 16702312]
• Lang CH. Elevated plasma free fatty acids decrease basal protein synthesis, but not the anabolic effect of leucine, in skeletal muscle. Am J Physiol Endocrinol Metab 2006-09-01 [PMID: 16684854]
• Lynch CJ, Gern B, Lloyd C et al. Leucine in food mediates some of the postprandial rise in plasma leptin concentrations. Am J Physiol Endocrinol Metab 2006-09-01 [PMID: 16638821]
• Escobar J, Frank JW, Suryawan A et al. Regulation of cardiac and skeletal muscle protein synthesis by individual branched-chain amino acids in neonatal pigs. Am J Physiol Endocrinol Metab 2006-04-01 [PMID: 16278252]
• Vary TC. IGF-I stimulates protein synthesis in skeletal muscle through multiple signaling pathways during sepsis. Am J Physiol Regul Integr Comp Physiol 2006-02-01 [PMID: 16150839]
• Krawiec BJ, Frost RA, Vary TC et al. Hindlimb casting decreases muscle mass in part by proteasome-dependent proteolysis but independent of protein synthesis. Am J Physiol Endocrinol Metab 2005-12-01 [PMID: 16046454]
• Crozier SJ, Vary TC, Kimball SR et al. Cellular energy status modulates translational control mechanisms in ischemic-reperfused rat hearts. Am J Physiol Heart Circ Physiol 2005-09-01 [PMID: 15894572]
• Hong-Brown LQ, Pruznak AM, Frost RA et al. Indinavir alters regulators of protein anabolism and catabolism in skeletal muscle. Am J Physiol Endocrinol Metab 2005-09-01 [PMID: 15827064]
• Williamson DL, Kimball SR, Jefferson LS et al. Acute treatment with TNF-alpha attenuates insulin-stimulated protein synthesis in cultures of C2C12 myotubes through a MEK1-sensitive mechanism. Am J Physiol Endocrinol Metab 2005-07-01 [PMID: 15701678]
• Dufner DA, Bederman IR, Brunengraber DZ et al. Using 2H2O to study the influence of feeding on protein synthesis: effect of isotope equilibration in vivo vs. in cell culture. Am J Physiol Endocrinol Metab 2005-06-01 [PMID: 15671077]
• Escobar J, Frank JW, Suryawan A et al. Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation. Am J Physiol Endocrinol Metab 2005-05-01 [PMID: 15644455]
• Reiter AK, Bolster DR, Crozier SJ et al. Repression of protein synthesis and mTOR signaling in rat liver mediated by the AMPK activator aminoimidazole carboxamide ribonucleoside. Am J Physiol Endocrinol Metab 2005-05-01 [PMID: 15613684]
• Kubica N, Bolster DR, Farrell PA et al. Resistance exercise increases muscle protein synthesis and translation of eukaryotic initiation factor 2Bepsilon mRNA in a mammalian target of rapamycin-dependent manner. J Biol Chem 2005-03-01 [PMID: 15591312]
• Anthony TG, McDaniel BJ, Byerley RL et al. Preservation of liver protein synthesis during dietary leucine deprivation occurs at the expense of skeletal muscle mass in mice deleted for eIF2 kinase GCN2. J Biol Chem 2004-08-01 [PMID: 15213227]
• Lang CH, Frost RA. Differential effect of sepsis on ability of leucine and IGF-I to stimulate muscle translation initiation. Am J Physiol Endocrinol Metab 2004-10-01 [PMID: 15186995]
• Kimball SR, Horetsky RL, Jefferson LS et al. A microtiter plate assay for assessing the interaction of eukaryotic initiation factor eIF4E with eIF4G and eIF4E binding protein-1. Anal Biochem 2004-02-01 [PMID: 14751272]
• Lang CH, Frost RA, Deshpande N et al. Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle. Am J Physiol Endocrinol Metab 2003-12-01 [PMID: 12944322]

Bioinformatics

• Gene Symbol: EIF4EBP1
• Entrez:
  • 1978(Human)
  • 13685(Mouse)
  • 116636(Rat)
• Uniprot:
  • Q13541()