Genetic Strategies: Knockout Validated: 4EBP1 Antibody [NB200-157] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 4EBP1 knockout (KO) HeLa cell line. PVDF membrane ...read more
Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using ...read more
The immunogen recognized by this antibody maps to a region between residue 75 and the C-terminus (residue 118) of human eukaryotic translation initiation factor 4E binding protein 1 using the numbering given in entry NP_004086.1 (GeneID 1978).
Immunogen affinity purified
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eukaryotic translation initiation factor 4E binding protein 1,4EBP1,4E-BP1BP-1
eukaryotic translation initiation factor 4E-binding protein 1
Phosphorylated heat- and acid-stable protein regulated by insulin 1
eIF4E is a protein that modulates the translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. . Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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