Ribosomal Protein S6/RPS6 Antibody

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Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells ...read more
Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB
Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells ...read more
Loss of SPAG5 and CLUH lead to hyperactivation of mTORC1 signaling. (C) Western blots of WT and SPAG5 ind-KO HeLa cells transfected with siRNA against CLUH or untargeted control siRNA. Cells were induced for 4 days with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, XpSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Ribosomal Protein S6/RPS6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and the C-terminus (residue 249) of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194).
Predicted Species
Rat (100%), Chicken (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-1595 in the following applications:

Publications
Read Publications using
NB100-1595 in the following applications:

  • IF
    1 publication
  • WB
    5 publications

Reactivity Notes

X. laevis (100%).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribosomal Protein S6/RPS6 Antibody

  • Phosphoprotein NP33, 40S ribosomal protein S6
  • pS6
  • Ribosomal Protein S6
  • RPS6
  • S6

Background

Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA. Alternate names for RPS6 include 40S ribosomal protein S6, and phosphoprotein NP33.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IF, WB.


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IF
(1)
WB
(5)
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Human
(1)
Mouse
(4)
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Showing Publications 1 - 6 of 6.
Publications using NB100-1595 Applications Species
De Luca C, Gupta A, Bortvin A Retrotransposon LINE-1 bodies in the cytoplasm of piRNA-deficient mouse spermatocytes: Ribonucleoproteins overcoming the integrated stress response PLoS genetics 2023-06-01 [PMID: 37307272] (IF, WB, Mouse) IF, WB Mouse
Schatton D, Di Pietro G, Szczepanowska K et al. CLUH controls astrin-1 expression to couple mitochondrial metabolism to cell cycle progression eLife 2022-05-13 [PMID: 35559794]
Zaganjor E, Yoon H, Spinelli JB et al. SIRT4 is an early regulator of branched-chain amino acid catabolism that promotes adipogenesis Cell reports 2021-07-13 [PMID: 34260923] (WB) WB
Pla-Martin D, Schatton D, Wiederstein Jl et Al. CLUH granules coordinate translation of mitochondrial proteins with mTORC1 signaling and mitophagy EMBO J. 2020-03-09 [PMID: 32149416] (WB, Mouse) WB Mouse
Gao J, Schatton D, Martinelli P et al. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. CLUH regulates mitochondrial biogenesis by binding mRnAs of nuclear-encoded mitochondrial proteins. 2014-10-27 [PMID: 25349259] (WB, Human, Mouse) WB Human, Mouse
Castaneda J, Genzor P, van der Heijden GW et al. Reduced pachytene piRNAs and translation underlie spermiogenic arrest in Maelstrom mutant mice. EMBO J. 2014-07-25 [PMID: 25063675] (WB, Mouse)

Details:
RPS6 antibody/rabbit anti-RPS6 used in WB assay to verify sucrose gradient performance in sucrose density gradient sedimentation profiles testing of MAEL (Maelstrom) and MIWI (Piwi partner of pachytene piRNAs) in native protein lysates from adult WT mouse testes - puromycin/untreated and EDTA/untreated samples (Figure 8A, B, C).
WB Mouse

Review for Ribosomal Protein S6/RPS6 Antibody (NB100-1595) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1595:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Ribosomal Protein S6/RPS6 NB100-1595
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4
reviewed by:
Verified Customer
WB Human 09/18/2015
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Summary

ApplicationWestern Blot
Sample TestedHepG2
SpeciesHuman
LotA1

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Verified Customer
09/18/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RPS6
Entrez
Uniprot