Ribosomal Protein S6/RPS6 Antibody

Images

 
Independent Antibodies: Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of Human RPS6 on HeLa whole cell lysate using NB100-1595. RPS6 was also immunoprecipitated using rabbit anti-RPS6 ...read more
Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RPS6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Ribosomal Protein S6/RPS6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 200 and the C-terminus (residue 249) of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPS6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-1595 in the following applications:

Publications
Read Publications using
NB100-1595 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribosomal Protein S6/RPS6 Antibody

  • Phosphoprotein NP33, 40S ribosomal protein S6
  • pS6
  • Ribosomal Protein S6
  • RPS6
  • S6

Background

Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA. Alternate names for RPS6 include 40S ribosomal protein S6, and phosphoprotein NP33.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-607
Species: Hu, Rt
Applications: WB, EM, ELISA, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF8962
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-89647
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF1518
Species: Hu, Mu, Rt
Applications: WB, IHC
NBP2-19804
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-38535
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-90149
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF6696
Species: Hu, Mu, Rt
Applications: WB, ICC
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)(2)

Review for Ribosomal Protein S6/RPS6 Antibody (NB100-1595) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-1595:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ribosomal Protein S6/RPS6 NB100-1595
Enlarge
reviewed by:
Anonymous
WB Human 09/18/2015
View

Summary

ApplicationWestern Blot
Sample TestedHepG2
SpeciesHuman
LotA1

Blocking

Blocking DetailsOdyssey Blocking Buffer (PBS) (Licor, part No. 927-40000), 1 h at room temperature

Primary Anitbody

Dilution Ratio1:1000 in 1:1 diluted Odyssey blocker : PBST (0.1% Tween 20 in PBS)

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, IRDye 800CW
Secondary Manufacturer Cat#(Licor 926-32211)
Secondary Concentration1:15000 in 1:1 Odyssey blocker:PBST

Details

Detection NotesLicor Odyssey CLx infrared scanner

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ribosomal Protein S6/RPS6 Antibody (NB100-1595) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ribosomal Protein S6/RPS6 Products

Bioinformatics Tool for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)

Discover related pathways, diseases and genes to Ribosomal Protein S6/RPS6 Antibody (NB100-1595). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)

Discover more about diseases related to Ribosomal Protein S6/RPS6 Antibody (NB100-1595).
 

Pathways for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)

View related products by pathway.

PTMs for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)

Learn more about PTMs related to Ribosomal Protein S6/RPS6 Antibody (NB100-1595).
 

Research Areas for Ribosomal Protein S6/RPS6 Antibody (NB100-1595)

Find related products by research area.

Blogs on Ribosomal Protein S6/RPS6

There are no specific blogs for Ribosomal Protein S6/RPS6, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
09/18/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol RPS6
Entrez
Uniprot