MNK1 Antibody (2F12) - Azide and BSA Free

MNK1-Antibody-2F12-Western-Blot-H00008569-M14-img0010.jpg

Immunocytochemistry/Immunofluorescence: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of monoclonal antibody to MKNK1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - detection against Immunogen (77.26 KDa) .

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - Analysis of MKNK1 expression in Raw 264.7. (Isoform: 51.342, 47.402, 39.103 kD)

Western Blot: MNK1 Antibody (2F12) [H00008569-M14] - p38 alpha -mediated phosphorylation pathways.a. Old wild type & p38 alpha knock out livers were Western blotted for phosphorylated MKK3/6 & MKK4, for phosphorylated p38 & total levels of p38 alpha . Densitometric quantification of p-MKK3/6 (S189/207)/ alpha -tubulin, p-MKK4 (S257/T261)/a tubulin were done. b. Old wild type & p38 alpha knock out livers were Western blotted for MNK1 & phosphorylated MNK1 on Thr197/202, for MK2 & phosphorylated MK2 on Thr334 & Thr222, for AKT & phosphorylated AKT on Ser473, for GSK3 beta & phosphorylated GSK3 beta on serine 9 & for HSP27 & phosphorylated HSP27 on Ser 82. alpha -tubulin was used as a loading control. Densitometric quantification of p-MNK-1(T197/202)/MNK1, p-MK2 (T334)/MK2, p-MK2 (T222) /MK2, p-AKT(S473)/AKT, p-GSK3 beta (S9)/GSK3 beta & p-HSP27(S82)/HSP27 were determined. Data are shown as mean ± SD. **P < 0.01 WT versus KO. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0171738), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MNK1 Antibody (2F12) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
• Specificity: MAP kinase interacting serine/threonine kinase 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MKNK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MNK1 Antibody (2F12) - Azide and BSA Free

• EC 2.7.11
• EC 2.7.11.1
• MAP kinase interacting kinase 1
• MAP kinase interacting serine/threonine kinase 1
• MAP kinase signal-integrating kinase 1
• MAP kinase-interacting serine/threonine-protein kinase 1
• MKNK1
• MNK1
• MNK1MAP kinase-interacting serine/threonine kinase 1

Background

MNK1 may play a role in the response to environmental stress and cytokines. It appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MNK1 Antibody (H00008569-M14)(1)

Publication using H00008569-M14

• Tormos AM, Rius-Perez S, Jorques M et al. p38alpha regulates actin cytoskeleton and cytokinesis in hepatocytes during development and aging. PLoS ONE. 2017-02-06 [PMID: 28166285] (WB, Mouse)

Bioinformatics

• Gene Symbol: MKNK1
• Entrez:
  • 8569(Human)
• OMIM:
  • 606724(Human)
• Uniprot:
  • AAH02755(Human)