PABP Antibody (10E10)

Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.

Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.

Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.

• Reactivity: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
• Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
• Clone: 10E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PABP Antibody (10E10) Summary

• Immunogen: Recombinant human PABP protein. [Uniprot# P11940]
• Localization: Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PABPC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • CyTOF-ready
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate

Reactivity Notes

Human, Monkey, Bovine, Dog, and Hamster and Xenopus laevis. Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PABP Antibody (10E10)

• PAB1polyadenylate-binding protein 1
• PABP
• PABP-1
• PABP1poly(A) binding protein, cytoplasmic 2
• PABPC2
• PABPL1
• poly(A) binding protein, cytoplasmic 1
• Poly(A)-binding protein 1
• poly(A)-binding protein, cytoplasmic 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PABP Antibody (NB120-6125)(7)

Publications using NB120-6125

• Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013 Mar 25 [PMID: 23530232] (WB, Human)
• Sagliocco F, Laloo B, Cosson B et al. The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP. Biochem J. 2006 Dec 1 [PMID: 16834569] (WB, Mouse)
• Larralde O, Smith RW, Wilkie GS et al. Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein. J Virol. 2006 Feb [PMID: 16415034] (WB, Human)
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004 Mar [PMID: 14970384] (WB, Human)
• Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ. Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4. Eur J Biochem. 2004 Jan [PMID: 14717712] (IP, Human)
• Lee J, Bedford MT. PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Rep. 2002 Mar [PMID: 11850402] (IP, Human)
• Grosset C, Chen CY, Xu N et al. A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. Cell. 2000 Sep 29 [PMID: 11051545] (WB, Mouse)

Review for PABP Antibody (NB120-6125) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rainbow trout, Oncorhynchus mykiss, Human.

reviewed by: Stephanie DeWitte-Orr WB Rainbow trout, Oncorhynchus mykiss, Human 05/02/2017

Summary

• Application: Western Blot
• Sample Tested: RTG-2 cells total lysate,HFF-1 cells total lysate
• Species: Rainbow trout, Oncorhynchus mykiss, Human
• Lot: A-1

Bioinformatics

• Gene Symbol: PABPC1
• Entrez:
  • 26986(Human)
• Uniprot:
  • P11940(Human)