PABP Antibody (10E10) - BSA Free

Images

 
Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA ...read more
Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
Clone
10E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

PABP Antibody (10E10) - BSA Free Summary

Immunogen
Recombinant human PABP protein. [Uniprot# P11940]
Localization
Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PABPC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • Western Blot 1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
A-431 Whole Cell Lysate
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB120-6125 in the following applications:

Publications
Read Publications using
NB120-6125 in the following applications:

  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, Monkey, Bovine, Dog, and Hamster and Xenopus laevis. Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PABP Antibody (10E10) - BSA Free

  • PAB1polyadenylate-binding protein 1
  • PABP
  • PABP-1
  • PABP1poly(A) binding protein, cytoplasmic 2
  • PABPC2
  • PABPL1
  • poly(A) binding protein, cytoplasmic 1
  • Poly(A)-binding protein 1
  • poly(A)-binding protein, cytoplasmic 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-268
Species: Hu, Po
Applications: IP, WB
NB100-81898
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-24529
Species: Ca, Ch, Hu, Mu, Pm
Applications: IHC, IHC-P, Simple Western, WB
NBP2-38802
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00010605-M04
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP2-67015
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-24632
Species: Ca, Ch, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
NB100-74593
Species: Hu, Mu
Applications: IHC, IHC-Fr (-), IHC-P, IP, Single-Cell Western, WB
NBP1-31113
Species: Hu, Pm, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB200-157
Species: Hu
Applications: IHC, IHC-P, KO, WB
NBP1-90063
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-97572
Species: Hu, Mu, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, IP, In vivo, KD, Simple Western, WB
NB100-1591
Species: Hu, Mu
Applications: IHC, IHC-P, IP, KD, WB
NBP2-37488
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-28751
Species: Hu, Mu
Applications: IB, IP, KD, WB
NBP2-86906
Species: Hu
Applications: IHC, IHC-P, WB
AF5059
Species: Hu
Applications: IHC, WB
NBP2-93867
Species: Hu, Mu, Rt
Applications: WB
NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready

Publications for PABP Antibody (NB120-6125)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(2)
WB
(5)
All Applications
Filter By Species
Human
(5)
Mouse
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB120-6125 Applications Species
Genois MM, Gagné JP, Yasuhara T, et al. CARM1 regulates replication fork speed and stress response by stimulating PARP1 Molecular cell Dec 30 2020 [PMID: 33412112]
Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013 Mar 25 [PMID: 23530232] (WB, Human) WB Human
Sagliocco F, Laloo B, Cosson B et al. The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP. Biochem J. 2006 Dec 1 [PMID: 16834569] (WB, Mouse) WB Mouse
Larralde O, Smith RW, Wilkie GS et al. Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein. J Virol. 2006 Feb [PMID: 16415034] (WB, Human) WB Human
Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004 Mar [PMID: 14970384] (WB, Human) WB Human
Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ. Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4. Eur J Biochem. 2004 Jan [PMID: 14717712] (IP, Human) IP Human
Lee J, Bedford MT. PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Rep. 2002 Mar [PMID: 11850402] (IP, Human) IP Human
Grosset C, Chen CY, Xu N et al. A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. Cell. 2000 Sep 29 [PMID: 11051545] (WB, Mouse) WB Mouse

Review for PABP Antibody (NB120-6125) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human, Oncorhynchus mykiss, Rainbow trout.

Reviews using NB120-6125:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human, Oncorhynchus mykiss, Rainbow trout
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PABP NB120-6125
Enlarge
5
reviewed by:
Stephanie DeWitte-Orr
WB Human, Oncorhynchus mykiss, Rainbow trout 05/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedHFF-1 cells total lysate,RTG-2 cells total lysate
SpeciesHuman, Oncorhynchus mykiss, Rainbow trout
LotA-1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PABP Antibody (NB120-6125) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PABP Products

Bioinformatics Tool for PABP Antibody (NB120-6125)

Discover related pathways, diseases and genes to PABP Antibody (NB120-6125). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PABP Antibody (NB120-6125)

Discover more about diseases related to PABP Antibody (NB120-6125).
 

Pathways for PABP Antibody (NB120-6125)

View related products by pathway.

PTMs for PABP Antibody (NB120-6125)

Learn more about PTMs related to PABP Antibody (NB120-6125).
 

Research Areas for PABP Antibody (NB120-6125)

Find related products by research area.

Blogs on PABP

There are no specific blogs for PABP, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Stephanie DeWitte-Orr
05/02/2017
Application: WB
Species: Human, Oncorhynchus mykiss, Rainbow trout

Bioinformatics

Gene Symbol PABPC1
Entrez
Uniprot