PABP Antibody (10E10)

Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.

Immunocytochemistry/Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.

Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.

• Reactivity: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
• Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
• Clone: 10E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PABP Antibody (10E10) Summary

• Immunogen: Recombinant human PABP protein. [Uniprot# P11940]
• Localization: Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PABPC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • CyTOF-ready
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  A-431 Lysate (NB800-PC12)
  HeLa Lysate (NB800-PC1)

Reactivity Notes

Human, Monkey, Bovine, Dog, and Hamster and Xenopus laevis. Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PABP Antibody (10E10)

• PAB1polyadenylate-binding protein 1
• PABP
• PABP-1
• PABP1poly(A) binding protein, cytoplasmic 2
• PABPC2
• PABPL1
• poly(A) binding protein, cytoplasmic 1
• Poly(A)-binding protein 1
• poly(A)-binding protein, cytoplasmic 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PABP Antibody (NB120-6125)(7)

Publications using NB120-6125

• Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013 Mar 25 [PMID: 23530232] (WB, Human)
• Sagliocco F, Laloo B, Cosson B et al. The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP. Biochem J. 2006 Dec 1 [PMID: 16834569] (WB, Mouse)
• Larralde O, Smith RW, Wilkie GS et al. Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein. J Virol. 2006 Feb [PMID: 16415034] (WB, Human)
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA. 2004 Mar [PMID: 14970384] (WB, Human)
• Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ. Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4. Eur J Biochem. 2004 Jan [PMID: 14717712] (IP, Human)
• Lee J, Bedford MT. PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays. EMBO Rep. 2002 Mar [PMID: 11850402] (IP, Human)
• Grosset C, Chen CY, Xu N et al. A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex. Cell. 2000 Sep 29 [PMID: 11051545] (WB, Mouse)

Review for PABP Antibody (NB120-6125) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rainbow trout, Oncorhynchus mykiss, Human.

reviewed by: Stephanie DeWitte-Orr WB Rainbow trout, Oncorhynchus mykiss, Human 05/02/2017

Summary

• Application: Western Blot
• Sample Tested: RTG-2 cells total lysate,HFF-1 cells total lysate
• Species: Rainbow trout, Oncorhynchus mykiss, Human
• Lot: A-1

Bioinformatics

• Gene Symbol: PABPC1
• Entrez:
  • 26986(Human)
• Uniprot:
  • P11940(Human)