PABP Antibody (10E10)

Images

 
Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA ...read more
Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
Clone
10E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PABP Antibody (10E10) Summary

Immunogen
Recombinant human PABP protein. [Uniprot# P11940]
Localization
Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PABPC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • CyTOF-ready
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
A-431 Lysate (NB800-PC12)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NB120-6125 in the following applications:

Publications
Read Publications using
NB120-6125 in the following applications:

  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, Monkey, Bovine, Dog, and Hamster and Xenopus laevis. Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PABP Antibody (10E10)

  • PAB1polyadenylate-binding protein 1
  • PABP
  • PABP-1
  • PABP1poly(A) binding protein, cytoplasmic 2
  • PABPC2
  • PABPL1
  • poly(A) binding protein, cytoplasmic 1
  • Poly(A)-binding protein 1
  • poly(A)-binding protein, cytoplasmic 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready

Publications for PABP Antibody (NB120-6125)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(2)
WB
(5)
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Filter By Species
Human
(5)
Mouse
(2)
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Review for PABP Antibody (NB120-6125) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rainbow trout, Oncorhynchus mykiss, Human.

Reviews using NB120-6125:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rainbow trout, Oncorhynchus mykiss, Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PABP NB120-6125
Enlarge
reviewed by:
Stephanie DeWitte-Orr
WB Rainbow trout, Oncorhynchus mykiss, Human 05/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedRTG-2 cells total lysate,HFF-1 cells total lysate
SpeciesRainbow trout, Oncorhynchus mykiss, Human
LotA-1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PABP Antibody (NB120-6125) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-6125AF405
Alexa Fluor 488 Labeled NB120-6125AF488
Alexa Fluor 647 Labeled NB120-6125AF647
Alexa Fluor 700 Labeled NB120-6125AF700
Allophycocyanin Labeled NB120-6125APC
DyLight 350 Labeled NB120-6125UV
DyLight 405 Labeled NB120-6125V
DyLight 488 Labeled NB120-6125G
DyLight 680 Labeled NB120-6125FR
DyLight 755 Labeled NB120-6125IR
PE Labeled NB120-6125PE

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Bioinformatics Tool for PABP Antibody (NB120-6125)

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Blogs on PABP

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Stephanie DeWitte-Orr
05/02/2017
Application: WB
Species: Rainbow trout, Oncorhynchus mykiss, Human

Bioinformatics

Gene Symbol PABPC1
Entrez
Uniprot