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PABP Antibody (10E10)

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Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Immunocytochemistry/ Immunofluorescence: PABP Antibody (10E10) [NB120-6125] - PABP antibody was tested in HeLa cells at a 1:50 dilution using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA ...read more
Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase ...read more

Product Details

Summary
Reactivity Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
Clone
10E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-6125AF350
Alexa Fluor 405
NB120-6125AF405
Alexa Fluor 488
NB120-6125AF488
Alexa Fluor 532
NB120-6125AF532
Alexa Fluor 594
NB120-6125AF594
Alexa Fluor 647
NB120-6125AF647
Alexa Fluor 700
NB120-6125AF700
Alexa Fluor 750
NB120-6125AF750
Allophycocyanin
NB120-6125APC
Biotin
NB120-6125B
DyLight 350
NB120-6125UV
DyLight 405
NB120-6125V
DyLight 488
NB120-6125G
DyLight 550
NB120-6125R
DyLight 594
NB120-6125DL594
DyLight 650
NB120-6125C
DyLight 680
NB120-6125FR
DyLight 755
NB120-6125IR
HRP
NB120-6125H
Janelia Fluor 549
NB120-6125JF549
Janelia Fluor 646
NB120-6125JF646
PE
NB120-6125PE
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80901
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PABP Antibody (10E10) Summary

Immunogen
Recombinant human PABP protein. [Uniprot# P11940]
Localization
Cytoplasmic. Shuttles between the cytoplasm and the nucleus.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PABPC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Microarray
  • CyTOF-ready
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
A-431 Whole Cell Lysate
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB120-6125 in the following applications:
Publications
Read Publications using
NB120-6125 in the following applications:

  • IP
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, Monkey, Bovine, Dog, and Hamster and Xenopus laevis. Does not cross-react with Drosophilia melanogaster or Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PABP Antibody (10E10)

  • PAB1polyadenylate-binding protein 1
  • PABP
  • PABP-1
  • PABP1poly(A) binding protein, cytoplasmic 2
  • PABPC2
  • PABPL1
  • poly(A) binding protein, cytoplasmic 1
  • Poly(A)-binding protein 1
  • poly(A)-binding protein, cytoplasmic 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody.
Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) antibody
Immunohistochemistry: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho-Ser209) antibody
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Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.
Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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Immunocytochemistry/Immunofluorescence: PAIP2 Antibody [NBP2-38802] - Immunofluorescent staining of human cell line HeLa shows localization to vesicles.
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Western Blot: PAIP1 Antibody [NBP1-57310] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml
Immunohistochemistry-Paraffin: PAIP1 Antibody [NBP1-57310] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Immunohistochemistry-Paraffin: PAIP1 Antibody [NBP1-57310] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
Species: Hu
Applications: WB, IHC, IHC-P
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Western Blot: PABPN1 Antibody (JM11-28) [NBP2-67015] - Analysis of PABPN1 on MCF-7 cells lysates using anti-PABPN1 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: PABPN1 Antibody (JM11-28) [NBP2-67015] - Staining PABPN1 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PABPN1 Antibody (JM11-28) [NBP2-67015] - Analysis of paraffin-embedded mouse testis tissue using anti-PABPN1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu
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Western Blot: eIF4A1 Antibody [NBP2-24632] - Analysis of eIF4A1 in K562 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) RAW cell lysate in the absence of immunizing peptide using NBP2-24632 at 0.25 ug/ml.
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Western Blot: PABPC4 Antibody [NB100-74593] - Detection of PABP4 on HeLa whole cell lysate (5, 15, and 50 microgram for WB; 1mg for IP, 20% of IP loaded), 293T (T; 50 microgram) and mouse NIH3T3 (M; 50 microgram) using NB100-74593. PABP4 was also immunoprecipitated by rabbit anti-PABP4 antibody NB100-74594.
Immunohistochemistry-Paraffin: PABPC4 Antibody [NB100-74593] - Section of human prostate carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- PABP4 used at a dilution of 1:200 (1ug/ml). Detection: DAB
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Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31113] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eRF1 protein stained by eRF1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000. Blue: Hoechst 33342 staining.
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(1 Review)
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Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP‑2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP‑2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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SDS-Page: Recombinant Human RNPC3 Protein [H00055599-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
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PABP Antibody
(10E10)
NB120-6125
PABP Antibody (10E10)
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready

Publications for PABP Antibody (NB120-6125)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.


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(2)
WB
(5)
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Mouse
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Review for PABP Antibody (NB120-6125) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rainbow trout, Oncorhynchus mykiss, Human.

Reviews using NB120-6125:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rainbow trout, Oncorhynchus mykiss, Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PABP NB120-6125
Enlarge
reviewed by:
Stephanie DeWitte-Orr
 WB Rainbow trout, Oncorhynchus mykiss, Human 05/02/2017
View

Summary

ApplicationWestern Blot
Sample TestedRTG-2 cells total lysate,HFF-1 cells total lysate
SpeciesRainbow trout, Oncorhynchus mykiss, Human
LotA-1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PABP Antibody (NB120-6125) (0)

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Control Lysate(s)

A-431 Whole Cell Lysate
HeLa Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB120-6125AF350
Alexa Fluor 405 NB120-6125AF405
Alexa Fluor 488 NB120-6125AF488
Alexa Fluor 532 NB120-6125AF532
Alexa Fluor 594 NB120-6125AF594
Alexa Fluor 647 NB120-6125AF647
Alexa Fluor 700 NB120-6125AF700
Alexa Fluor 750 NB120-6125AF750
Allophycocyanin NB120-6125APC
Biotin NB120-6125B
DyLight 350 NB120-6125UV
DyLight 405 NB120-6125V
DyLight 488 NB120-6125G
DyLight 550 NB120-6125R
DyLight 594 NB120-6125DL594
DyLight 650 NB120-6125C
DyLight 680 NB120-6125FR
DyLight 755 NB120-6125IR
HRP NB120-6125H
Janelia Fluor 549 NB120-6125JF549
Janelia Fluor 646 NB120-6125JF646
PE NB120-6125PE
Learn about Custom Antibody Labeling

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Western Blot PABP NB120-6125
Enlarge
 Stephanie DeWitte-Orr
05/02/2017
Application: WB
Species: Rainbow trout, Oncorhynchus mykiss, Human
read this review

Bioinformatics

Gene Symbol PABPC1
Entrez
Uniprot
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