eIF4G1 Antibody

Images

 
Western Blot: eIF4G1 Antibody [NB100-268] - Rat liver lysate [10K x g supernatant, ~50 ug (left) or 150 ug (right lane)] separated on 7.5% gel and transferred to PVDF membranes.Antibody: Affinity purified rabbit ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

eIF4G1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 575 and 625 of human Eukaryotic Initiation Factor 4 Gamma 1 (NP_886553.2).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF4G1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1 - 4 ug/mg of lysate
Application Notes
EIF-4G1 migrates in SDS-PAGE just above myosin standards.
Publications
Read Publications using
NB100-268 in the following applications:

  • WB
    2 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 22013195).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF4G1 Antibody

  • DKFZp686A1451
  • EIF4F
  • eIF-4G 1
  • eIF4G1
  • EIF-4G1
  • eIF-4-gamma 1
  • EIF4-gamma
  • EIF4GI
  • EIF4Gp220
  • eucaryotic translation initiation factor 4G
  • eukaryotic translation initiation factor 4 gamma 1
  • eukaryotic translation initiation factor 4 gamma, 1
  • P220

Background

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for eIF4G1 Antibody (NB100-268)(25)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Porcine
(2)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-268 Applications Species
Hernandez-Garcia AD, Columbus DA, Manjarin R et al. Leucine supplementation stimulates protein synthesis and reduces degradation signal activation in muscle of newborn pigs during acute endotoxemia Am. J. Physiol. Endocrinol. Metab. Sep 13 2016 [PMID: 27624100] (WB, Porcine) WB Porcine
Galicia-Vazquez G, Cencic R, Robert F et al. A cellular response linking eIF4AI activity to eIF4AII transcription. RNA 2012 Jul [PMID: 22589333]
El-Kadi SW, Suryawan A, Gazzaneo MC et al. Anabolic Signaling and Protein Deposition Are Enhanced by Intermittent as Compared with Continuous Feeding in Skeletal Muscle of Neonates. American Journal of Physiology, Endocrinology and Metabolism. 2012 [PMID: 22215651]
Gazzaneo MC, Suryawan A, Orellana RA et al. Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs J Nutr 2011 Dec [PMID: 22013195] (WB, Porcine) WB Porcine
Cencic R, Hall DR, Robert F et al. Reversing chemoresistance by small molecule inhibition of the translation initiation complex eIF4F. Proc Natl Acad Sci U S A 2011 Jan [PMID: 21191102]
Sharma A, Hoeffer CA, Takayasu Y et al. Dysregulation of mTOR signaling in fragile X syndrome. J Neurosci 2010 Jan [PMID: 20071534]
Chale-Rush A, Morris EP, Kendall TL et al. Effects of chronic overload on muscle hypertrophy and mTOR signaling in young adult and aged rats. J Gerontol A Biol Sci Med Sci 2009 Dec [PMID: 19808838]
Wilson FA, Suryawan A, Orellana RA et al. Feeding Rapidly Stimulates Protein Synthesis in Skeletal Muscle of Neonatal Pigs by Enhancing Translation Initiation. J Nutr;139(10):1873-1880. 2009 [PMID: 19692527]
Spurgeon ME, Ornelles DA. The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection. J Virol 2009 Oct [PMID: 19605483]
Suryawan A, Jeyapalan AS, Orellana RA et al. Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. Am J Physiol Endocrinol Metab 2008 Oct [PMID: 18682538]
Show All 25 Publications.

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Bioinformatics

Gene Symbol EIF4G1
Entrez
Uniprot