eIF4G1 Antibody


Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ...read more
Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI ...read more

Product Details

Reactivity Hu, Po, MuSpecies Glossary
Applications WB, IP
1.0 mg/ml

eIF4G1 Antibody Summary

The immunogen recognized by this antibody maps to a region between residues 575 and 625 of human Eukaryotic Initiation Factor 4 Gamma 1 (NP_886553.2).
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
Read Publications using
NB100-268 in the following applications:

  • WB
    3 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 22013195).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for eIF4G1 Antibody

  • DKFZp686A1451
  • EIF4F
  • eIF-4G 1
  • eIF4G1
  • EIF-4G1
  • eIF-4-gamma 1
  • EIF4-gamma
  • EIF4GI
  • EIF4Gp220
  • eucaryotic translation initiation factor 4G
  • eukaryotic translation initiation factor 4 gamma 1
  • eukaryotic translation initiation factor 4 gamma, 1
  • P220


eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
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Publications for eIF4G1 Antibody (NB100-268)(25)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: WB.

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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB100-268 Applications Species
Mendez-Solis O, Bendjennat M, Naipauer J et al. Kaposi's sarcoma herpesvirus activates the hypoxia response to usurp HIF2alpha-dependent translation initiation for replication and oncogenesis Cell reports 2021-12-28 [PMID: 34965440] (WB) WB
Hernandez-Garcia AD, Columbus DA, Manjarin R et al. Leucine supplementation stimulates protein synthesis and reduces degradation signal activation in muscle of newborn pigs during acute endotoxemia Am. J. Physiol. Endocrinol. Metab. 2016-09-13 [PMID: 27624100] (WB, Porcine) WB Porcine
Galicia-Vazquez G, Cencic R, Robert F et al. A cellular response linking eIF4AI activity to eIF4AII transcription. RNA 2012-07-01 [PMID: 22589333]
El-Kadi SW, Suryawan A, Gazzaneo MC et al. Anabolic Signaling and Protein Deposition Are Enhanced by Intermittent as Compared with Continuous Feeding in Skeletal Muscle of Neonates. American Journal of Physiology, Endocrinology and Metabolism. 2012-01-01 [PMID: 22215651]
Gazzaneo MC, Suryawan A, Orellana RA et al. Intermittent bolus feeding has a greater stimulatory effect on protein synthesis in skeletal muscle than continuous feeding in neonatal pigs J Nutr 2011-12-01 [PMID: 22013195] (WB, Porcine) WB Porcine
Cencic R, Hall DR, Robert F et al. Reversing chemoresistance by small molecule inhibition of the translation initiation complex eIF4F. Proc Natl Acad Sci U S A 2011-01-01 [PMID: 21191102]
Sharma A, Hoeffer CA, Takayasu Y et al. Dysregulation of mTOR signaling in fragile X syndrome. J Neurosci 2010-01-01 [PMID: 20071534]
Wilson FA, Suryawan A, Orellana RA et al. Feeding rapidly stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing translation initiation. J Nutr 2009-10-01 [PMID: 19692527]
Spurgeon ME, Ornelles DA. The adenovirus E1B 55-kilodalton and E4 open reading frame 6 proteins limit phosphorylation of eIF2alpha during the late phase of infection. J Virol 2009-10-01 [PMID: 19605483]
Suryawan A, Jeyapalan AS, Orellana RA et al. Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. Am J Physiol Endocrinol Metab 2008-10-01 [PMID: 18682538]
Show All 25 Publications.

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Gene Symbol EIF4G1