| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57716-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04611-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08883 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08883 | |||
![Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Western-Blot-NBP2-24529-img0001.jpg "Western Blot: eIF4A2 Antibody [NBP2-24529] - analysis of eIF4A2 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) 3T3 cell lysate using eIF4A2 antibody at 0.05 ug/ml, 0.05 ug/ml and 0.025 ug/ml, respectively.")
![Immunohistochemistry-Paraffin: eIF4A2 Antibody [NBP2-24529] - Formalin-fixed, paraffin-embedded human small intestine stained with eIF4A2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Immunohistochemistry-Paraffin-NBP2-24529-img0002.jpg)
![Simple Western: eIF4A2 Antibody [NBP2-24529] - Simple Western lane view shows a specific band for Eif4a2 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Simple-Western-NBP2-24529-img0003.jpg)
![Western Blot: eIF4A2 Antibody (PSH01-88) [NBP3-32300] - Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (NBP3-32300) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: C2C12 cell lysate Lane 3: COS-1 cell lysate Lane 4: Human brain tissue lysate Lane 5: Mouse testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46.4 kDa Observed band size: 46 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32300) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32300_rabbit-eif4a2-mab-psh01-88-278202415295556.jpg "Western Blot: eIF4A2 Antibody (PSH01-88) [NBP3-32300] - Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (NBP3-32300) at 1/1,000 dilution. Lane 1: PC-3M cell lysate Lane 2: C2C12 cell lysate Lane 3: COS-1 cell lysate Lane 4: Human brain tissue lysate Lane 5: Mouse testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46.4 kDa Observed band size: 46 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32300) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4A2 Antibody (PSH01-88) [NBP3-32300] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-eIF4A2 antibody (NBP3-32300) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32300) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32300_rabbit-eif4a2-mab-psh01-88-298202414262690.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4A2 Antibody (PSH01-88) [NBP3-32300] - Immunocytochemistry analysis of PC-12 cells labeling eIF4A2 with Rabbit anti-eIF4A2 antibody (NBP3-32300) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF4A2 antibody (NBP3-32300) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32300_rabbit-eif4a2-mab-psh01-88-29202410234258.jpg)
![Flow Cytometry: eIF4A2 Antibody (PSH01-88) [NBP3-32300] - Flow cytometric analysis of HeLa cells labeling eIF4A2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32300, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32300_rabbit-eif4a2-mab-psh01-88-29202416372120.jpg)
![Western Blot: eIF4A2 Antibody [NBP2-57716] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Western-Blot-NBP2-57716-img0002.jpg "Western Blot: eIF4A2 Antibody [NBP2-57716] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: eIF4A2 Antibody [NBP2-57716] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57716-img0001.jpg)
![Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301] - Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (NBP3-32301) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-3M cell lysate Lane 4: 293T cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: COS-1 cell lysate Lane 10: Human brain tissue lysate Lane 11: Mouse testis tissue lysate Lane 12: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32301) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32301_rabbit-eif4a2-mab-psh01-89-278202415212418.jpg "Western Blot: eIF4A2 Antibody (PSH01-89) [NBP3-32301] - Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (NBP3-32301) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-3M cell lysate Lane 4: 293T cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C2C12 cell lysate Lane 8: PC-12 cell lysate Lane 9: COS-1 cell lysate Lane 10: Human brain tissue lysate Lane 11: Mouse testis tissue lysate Lane 12: Rat testis tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32301) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: eIF4A2 Antibody [NBP3-04611] - Analysis of extracts of various cell lines, using eIF4A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/eIF4A2-Antibody-Western-Blot-NBP3-04611-img0004.jpg "Western Blot: eIF4A2 Antibody [NBP3-04611] - Analysis of extracts of various cell lines, using eIF4A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Western Blot: eIF4A2 Overexpression Lysate (Adult Normal) [NBL1-10202] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4A2.](http://images.novusbio.com/fullsize/eIF4A2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10202-img0002.jpg "Western Blot: eIF4A2 Overexpression Lysate (Adult Normal) [NBL1-10202] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4A2.")
![Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4A2 Mouse Monoclonal Antibody (PCRP-eIF4A2-2B5).](http://images.novusbio.com/fullsize/eIF4A2-Antibody-PCRP-EIF4A2-2B5-Protein-Array-NBP3-07140-img0001.jpg "Protein Array: eIF4A2 Antibody (PCRP-EIF4A2-2B5) [NBP3-07140] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4A2 Mouse Monoclonal Antibody (PCRP-eIF4A2-2B5).")
![ELISA: Human eIF4A2 ELISA Kit (Colorimetric) [NBP3-31554] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31554_human-eif4a2-elisa-kit-colorimetric-206202415294036.png "ELISA: Human eIF4A2 ELISA Kit (Colorimetric) [NBP3-31554] - Standard Curve Reference")
![ELISA: Human eIF4A2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31555] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31555_human-eif4a2-ready-to-use-elisa-kit-colorimetric-206202415314849.png "ELISA: Human eIF4A2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31555] - Standard Curve Reference")