E-Cadherin Antibody [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse ...read more
E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. ...read more
E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded ...read more
Retention of the spermatogonial phenotype following gene correction.Immunostaining was performed on gene-corrected GT59 (left) and GT65 cells (right): DAZL, a germ cell specific marker; GFRA1, POU5F1, ETV5, CDH1, and ...read more
Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each ...read more
Cellular localization of PEDF in testicular spermatogenic cells. Methanol-fixed cells were used to stain premeiotic and meiotic/postmeiotic cells as described in Figure 2. (A) alpha 6-integrin, (B) GFR-alpha , (C) ...read more
Testis morphogenesis of Mafb KO embryos developed normally.(A) Histological section of WT and KO E18.5 testes stained with HE. No morphological alteration or distribution was detectable. (B and C) Counts of Leydig and ...read more
Localization of MAFB in mouse testes.(A) Localization of MAFB in E18.5 mouse testes. Double immunostaining of MAFB with E-cadherin (ECAD), GATA4, or STAR is shown. Nuclei were counterstained with DAPI. The color of each ...read more
Testis morphogenesis of Mafb KO embryos developed normally.(A) Histological section of WT and KO E18.5 testes stained with HE. No morphological alteration or distribution was detectable. (B and C) Counts of Leydig and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

E-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709
Accession # P09803
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF748 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody [Unconjugated]

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha , beta , and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP3-25538
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-91662
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-15723
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
NBP1-54467
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-48309
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
NBP1-86509
Species: Hu
Applications: IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-85047
Species: Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-91258
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-03886
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
1129-ER
Species: Hu
Applications: BA
DVE00
Species: Hu
Applications: ELISA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF

Publications for E-Cadherin Antibody (AF748)(69)

We have publications tested in 5 confirmed species: Human, Mouse, Porcine, Transgenic Mouse, Xenopus.

We have publications tested in 10 applications: Confocal Microscopy, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Confocal Microscopy
(1)
Flow Cytometry
(1)
ICC
(12)
IF
(1)
IHC
(38)
IHC-Fr
(7)
IHC-P
(6)
Neutralization
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(14)
Mouse
(50)
Porcine
(1)
Transgenic Mouse
(4)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 69. Show All 69 Publications.
Publications using AF748 Applications Species
Liu, K;Tang, M;Xu, W;Meng, X;Jin, H;Han, M;Pu, J;Li, Y;Jiao, F;Sun, R;Shen, R;Lui, KO;Lu, L;Zhou, B; An inducible hACE2 transgenic mouse model recapitulates SARS-CoV-2 infection and pathogenesis in vivo Proceedings of the National Academy of Sciences of the United States of America 2023-06-20 [PMID: 37307455] (IHC, Transgenic Mouse) IHC Transgenic Mouse
Liu, X;Weng, W;He, L;Zhou, B; Genetic recording of in vivo cell proliferation by ProTracer Nature protocols 2023-06-02 [PMID: 37268780] (IHC, Mouse) IHC Mouse
Alvizi, L;Nani, D;Brito, LA;Kobayashi, GS;Passos-Bueno, MR;Mayor, R; Neural crest E-cadherin loss drives cleft lip/palate by epigenetic modulation via pro-inflammatory gene-environment interaction Nature communications 2023-05-24 [PMID: 37225711] (ICC, Human) ICC Human
Frum, T;Hsu, PP;Hein, RFC;Conchola, AS;Zhang, CJ;Utter, OR;Anand, A;Zhang, Y;Clark, SG;Glass, I;Sexton, JZ;Spence, JR; Opposing roles for TGF?- and BMP-signaling during nascent alveolar differentiation in the developing human lung bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205521] (IHC, Human) IHC Human
Lin, YH;Wei, Y;Zeng, Q;Wang, Y;Pagani, CA;Li, L;Zhu, M;Wang, Z;Hsieh, MH;Corbitt, N;Zhang, Y;Sharma, T;Wang, T;Zhu, H; IGFBP2 expressing midlobular hepatocytes preferentially contribute to liver homeostasis and regeneration Cell stem cell 2023-05-04 [PMID: 37146585] (IHC, Transgenic Mouse) IHC Transgenic Mouse
M Costa Fuji, A Yazdanpana, S Horne, A Lamont, P Lopez, C Farr Zuend, K Birse, M Taverner, R Greenslade, M Abou, L Noel-Romas, B Abrenica, O Ajibola, N Ikeogu, RC Su, LR McKinnon, H Pymar, V Poliquin, AR Berard, A Burgener, TT Murooka Non-optimal bacteria species induce neutrophil-driven inflammation and barrier disruption in the female genital tract Mucosal Immunology, 2023-04-29;0(0):. 2023-04-29 [PMID: 37121385] (IHC, Mouse) IHC Mouse
M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins bioRxiv : the preprint server for biology, 2023-04-04;0(0):. 2023-04-04 [PMID: 37066397] (IHC, Mouse) IHC Mouse
SK Russell, JK Harrison, BS Olson, HJ Lee, VP O'Brien, X Xing, J Livny, L Yu, EDO Roberson, R Bomjan, C Fan, M Sha, S Estfanous, AO Amer, M Colonna, TS Stappenbec, T Wang, TJ Hannan, SJ Hultgren Uropathogenic Escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome Nature Microbiology, 2023-04-10;0(0):. 2023-04-10 [PMID: 37037942] (ICC, Mouse) ICC Mouse
MC Paul, C Schneeweis, C Falcomatà, C Shan, D Rossmeisl, S Koutsouli, C Klement, M Zukowska, SA Widholz, M Jesinghaus, KK Heuermann, T Engleitner, B Seidler, K Sleiman, K Steiger, M Tschurtsch, B Walter, SA Weidemann, R Pietsch, A Schnieke, RM Schmid, MS Robles, G Andrieux, M Boerries, R Rad, G Schneider, D Saur Non-canonical functions of SNAIL drive context-specific cancer progression Nature Communications, 2023-03-07;14(1):1201. 2023-03-07 [PMID: 36882420] (ICC, Human) ICC Human
MC Paul, C Schneeweis, C Falcomatà, C Shan, D Rossmeisl, S Koutsouli, C Klement, M Zukowska, SA Widholz, M Jesinghaus, KK Heuermann, T Engleitner, B Seidler, K Sleiman, K Steiger, M Tschurtsch, B Walter, SA Weidemann, R Pietsch, A Schnieke, RM Schmid, MS Robles, G Andrieux, M Boerries, R Rad, G Schneider, D Saur Non-canonical functions of SNAIL drive context-specific cancer progression Nature Communications, 2023;14(1):1201. 2023 [PMID: 36882420] (ICC, Human) ICC Human
Show All 69 Publications.

Reviews for E-Cadherin Antibody (AF748) (0)

There are no reviews for E-Cadherin Antibody (AF748). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for E-Cadherin Antibody (AF748) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional E-Cadherin Products

Diseases for E-Cadherin Antibody (AF748)

Discover more about diseases related to E-Cadherin Antibody (AF748).
 

Pathways for E-Cadherin Antibody (AF748)

View related products by pathway.

PTMs for E-Cadherin Antibody (AF748)

Learn more about PTMs related to E-Cadherin Antibody (AF748).

Blogs on E-Cadherin.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and ...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our E-Cadherin Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol CDH1
Uniprot