E-Cadherin Antibody [Unconjugated]

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E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 °C. ...read more
E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in immersion fixed mouse intestinal organoids using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room ...read more
E‑Cadherin was detected in perfusion fixed frozen sections of mouse spinal cord using Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 1.7 µg/mL overnight at 4 ...read more
Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse ...read more
Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

E-Cadherin Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Cadherin
Asp157-Val709
Accession # P09803
Specificity
Detects mouse E-Cadherin in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDH1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF748 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Cadherin Antibody [Unconjugated]

  • Arc-1
  • CAD1
  • cadherin 1, E-cadherin (epithelial)
  • cadherin 1, type 1, E-cadherin (epithelial)
  • Cadherin-1
  • calcium-dependent adhesion protein, epithelial
  • CAM 120/80
  • CD324 antigen
  • CD324
  • CDH1
  • CDHE
  • cell-CAM 120/80
  • Cell-CAM120/80
  • ECAD
  • ECadherin
  • E-Cadherin
  • Epithelial cadherin
  • LCAM
  • L-CAM
  • UVOE-Cadherin
  • Uvomorulin

Background

Epithelial (E)‑Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) 251:1451.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Cadherin Antibody (AF748)(34)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: Confocal Microscopy, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Confocal Microscopy
(1)
Flow Cytometry
(1)
ICC
(5)
IHC
(10)
IHC-Fr
(6)
IHC-P
(5)
Neutralization
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(30)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using AF748 Applications Species
AD Werts, WB Fulton, MR Ladd, A Saad-Eldin, YX Chen, ML Kovler, H Jia, EC Banfield, R Buck, K Goerhing, T Prindle, S Wang, Q Zhou, P Lu, Y Yamaguchi, CP Sodhi, DJ Hackam A novel role for necroptosis in the pathogenesis of necrotizing enterocolitis Cell Mol Gastroenterol Hepatol, 2019;0(0):. 2019 [PMID: 31756560] (IHC, Mouse) IHC Mouse
C Liu, T Tate, E Batourina, ST Truschel, S Potter, M Adam, T Xiang, M Picard, M Reiley, K Schneider, M Tamargo, C Lu, X Chen, J He, H Kim, CL Mendelsohn Pparg promotes differentiation and regulates mitochondrial gene expression in bladder epithelial cells Nat Commun, 2019;10(1):4589. 2019 [PMID: 31597917] (IHC-P, Mouse) IHC-P Mouse
MK Yanda, B Cha, C Cebotaru, L Cebotaru Pharmacological reversal of renal cysts from secretion to absorption suggests a potential therapeutic strategy for managing polycystic kidney disease J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31570523] (Confocal Microscopy, Mouse) Confocal Microscopy Mouse
K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice Dis Model Mech, 2019;0(0):. 2019 [PMID: 31562139] (Western Blot, Mouse) Western Blot Mouse
DM Gay, RA Ridgway, M Müeller, MC Hodder, A Hedley, W Clark, JD Leach, R Jackstadt, C Nixon, DJ Huels, AD Campbell, TG Bird, OJ Sansom Loss of BCL9/9l suppresses Wnt driven tumourigenesis in models that recapitulate human cancer Nat Commun, 2019;10(1):723. 2019 [PMID: 30760720] (Proximity Ligation Assay (PLA), Mouse) Proximity Ligation Assay (PLA) Mouse
MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018;25(13):3811-3827.e7. 2018 [PMID: 30590051] (IHC-Fr, Mouse) IHC-Fr Mouse
VP O'Brien, DA Dorsey, TJ Hannan, SJ Hultgren Host restriction of Escherichia coli recurrent urinary tract infection occurs in a bacterial strain-specific manner PLoS Pathog., 2018;14(12):e1007457. 2018 [PMID: 30543708] (IHC-P, Mouse) IHC-P Mouse
S Flemming, AC Luissint, A Nusrat, CA Parkos Analysis of leukocyte transepithelial migration using an in vivo murine colonic loop model JCI Insight, 2018;3(20):. 2018 [PMID: 30333307] (IHC-P, Mouse) IHC-P Mouse
J Wang, E Batourina, K Schneider, S Souza, T Swayne, C Liu, CD George, T Tate, H Dan, G Wiessner, Y Zhuravlev, JC Canman, IU Mysorekar, CL Mendelsohn Polyploid Superficial Cells that Maintain the Urothelial Barrier Are Produced via Incomplete Cytokinesis and Endoreplication Cell Rep, 2018;25(2):464-477.e4. 2018 [PMID: 30304685] (IHC, Mouse) IHC Mouse
O Nekrasova, RM Harmon, JA Broussard, JL Koetsier, LM Godsel, GN Fitz, ML Gardel, KJ Green Desmosomal cadherin association with Tctex-1 and cortactin-Arp2/3 drives perijunctional actin polymerization to promote keratinocyte delamination Nat Commun, 2018;9(1):1053. 2018 [PMID: 29535305] (ICC, Human) ICC Human
Show All 34 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 FAB748R
Biotin BAF748

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Blogs on E-Cadherin.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

Beta-catenin - I am versatile!
Beta-catenin is a cytosolic, 88 kDa intracellular protein associated with cell surface cadherin glycoproteins. It is a member of the larger calcium-dependent catenin family that includes alpha-catenin, beta-catenin, and gamma-catenin (also known as pl...  Read full blog post.

Vimentin: Regulating EMT and Cancer
Vimentin, a member of the intermediate filament (IF) family, is a protein responsible for maintaining cellular integrity and reducing damage caused by stress. The vimentin protein is ubiquitously expressed in normal mesenchymal cells, and recent resea...  Read full blog post.

Carbonic Anhydrase IX Roles in Tumor Growth, Survival and Invasion
Carbonic anhydrase IX (CAIX) is a membrane-associated carbonic anhydrase, strongly induced by hypoxia. CA IX is overexpressed by several cancer cells from many tumor types, and is a component of the pH regulatory system invoked by these cells to comba...  Read full blog post.

E-Cadherin as a Cancer Biomarker
E-cadherin is a calcium-regulated adhesion molecule expressed in most normal epithelial tissues. E-cadherin is also associated with gland formation, stratification, and epithelial polarization, while loss of E-cadherin can cause dedifferentiation and...  Read full blog post.

E-Cadherin in Cell-Cell Adhesion and Cancer Diagnostics
E-Cadherin is a member of the cadherin superfamily and is fundamental player in a wide range of cellular processes such as development, morphology, polarity, migration and tissue integrity. Specifically, E-cadherin is an approximately 100 kDa epitheli...  Read full blog post.

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Bioinformatics

Gene Symbol CDH1
Uniprot