ITK Antibody

Western Blot: ITK Antibody [NB100-807] - Staining of Jurkat nuclear cell lysate with antibody at 1 ug/mL (A) and negative control Human parathyroid gland (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: ITK Antibody [NB100-807] - Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing strong cytoplasmic and weak nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Immunocytochemistry/Immunofluorescence: ITK Antibody [NB100-807] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ITK Antibody Summary

• Immunogen: Peptide with sequence C-RLLRQLAEIAESGL corresponding to C-Terminus according to NP_005537.3.
• Localization: Plasma membrane
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ITK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 75 kDa band bands observed in Jurkat Lysates.
• Control:

  Jurkat Whole Cell Lysate
  ITK Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ITK Antibody

• EC 2.7.10
• EC 2.7.10.2
• EMT
• EMTMGC126257
• homolog of mouse T-cell itk/tsk
• IL2-inducible T-cell kinase
• ITK
• Kinase EMT
• LYK
• LYKMGC126258
• PSCTK2
• T-cell-specific kinase
• Tcsk
• tyrosine-protein kinase ITK/TSK
• Tyrosine-protein kinase Lyk

Background

ITK is a T cell signaling protein. The Tec family of non receptor tyrosine kinases is composed of six proteins designated Tec, ITK (also known as Emt or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain pleckstrin homology (PH) and Tec homology (TH) domains in their amino termini. ITK associates with CD28 and becomes activated subsequent to CD28 ligation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ITK Antibody (NB100-807)(3)

Publications using NB100-807

• Sarkar R, Mondal PK, Datta M, Chatterjee BK. Note: A new optical method for the detection of bubble nucleation in superheated droplet detector. The Review of scientific instruments 2018-02-05 [PMID: 28667990]
• Willrich AH, Jung JE, Percicote AP et al. Adult rhabdomyoma at submandibular gland. Brazilian journal of otorhinolaryngology 2013-05-09 [PMID: 23108836]
• Tanaka N, Asao H, Ohtani K et al. A novel human tyrosine kinase gene inducible in T cells by interleukin 2. FEBS Lett 1993-06-07 [PMID: 8504851]

Bioinformatics

• Gene Symbol: ITK
• Entrez:
  • 3702(Human)
  • 16428(Mouse)
• Uniprot:
  • NP_005537(Human)