E-Cadherin Antibody (67A4) - BSA Free

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - A surface stain was performed on A549 cells with E-Cadherin Antibody (67A4) NBP1-42793AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) PE.

Flow Cytometry: E-Cadherin Antibody (67A4) [NBP1-42793] - Surface staining of HT-29 / SP2 cells with anti-CD324 (67A4) APC.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 67A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

E-Cadherin Antibody (67A4) - BSA Free Summary

• Description: Novus Biologicals Mouse E-Cadherin Antibody (67A4) - BSA Free (NBP1-42793) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-E-Cadherin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone 67A4 was used by HLDA to establish CD designation.
• Immunogen: T-47D cells
• Marker: Epithelial Cell Marker, Adherens Junctions Marker
• Specificity: The mouse monoclonal antibody 67A4 recognizes CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms. HLDA VIII
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 5-10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 4-8 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Flow Cytometry: Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 ug/ml per 1 million cells/ml. This antibody is CyTOF ready.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for E-Cadherin Antibody (67A4) - BSA Free

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Cadherin Antibody (NBP1-42793)(2)

Publications using NBP1-42793

• Lee, JS;Kweon, HJ;Lee, H;Suh, BC; Rapid resensitization of ASIC2a is conferred by three amino acid residues in the N terminus J. Gen. Physiol. 2019-04-22 [PMID: 31010811] (WB, Human)
• Kweon HJ, Cho JH, Jang IS, Suh BC. ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents BMB Rep 2016-10-01 [PMID: 27241858] (WB, Human)

Bioinformatics

• Gene Symbol: CDH1
• Entrez:
  • 999(Human)
• Uniprot:
  • P12830()