CXCR4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - Optimization of immunofluorescence staining of CXCR4 protein. MDA, PC3, KG1 cells were used for the optimization experiments. Cells were seeded onto cell-tak coated 48 mm coverslips in a 48-well plate. Cells were fixed with 2% formaldehyde for 20 min, washed with PBS. After fixation, cells were blocked with 2.0% BSA in PBS for 1 h at room temperature. All the cells were incubated with a primary antibody for anti-rabbit CXCR4 for 1 h. After washing with PBS, cells were put in respective secondary antibodies-anti-rabbit dylight 405 for 1 h. PSMA= Magenta, EpCAM= Yellow, sLex= Green, CXCR4= Red, and Merge shows all the colors. MDA=PSMA+, EpCAM+, sLex+, CXCR4+. PC3 = PSMA-, EpCAM+, sLex-, CXCR4+. KG1 = PSMA-, EpCAM-, sLex+, CXCR4-. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0085143) licensed under a CC-BY license.

Western Blot: CXCR4 Antibody [NB100-74396] - The A2AR and baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 protein expression levels in lung tissue were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC5543745/) licensed under a CC-BY license.

Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R54 in combination with nivolumab reduced the expression of CXCR4-CXCL12 and PD-L1 in PES43 tumors. Representative IHC pictures (magnification 400x) for CXCR4, CXCR7 (red staining), CXCL12, PD-1 and PD-L1 expression (brown staining) in PES43 collected tumors from mice treated with Pep R54, nivolumab or combined treatment. Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-019-1420-8) licensed under a CC-BY license.

Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R in combination with anti-PD-1 reduced the expression of CXCR4-CXCL12 and PD-L1 in MC38 tumors. Representative IHC pictures for CXCR4, CXCR7, CXCL12, PD-1 and PD-L1 expression (brown staining) in MC38 collected tumors (magnification 400x), from mice treated with Pep R, anti-murine PD-1 or combined treatment showing the reduction of CXCR4, CXCL12 and PD-L1 expression in mice treated with Pep R alone and in combination with anti-PD-1Image collected and cropped by CiteAb from the following publication (//jeccr.biomedcentral.com/articles/10.1186/s13046-019-1420-8) licensed under a CC-BY license.

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 protein in human small intestine tissue lysate using 1:500 dilution of rabbit polyclonal CXCR4 antibody (Lot A2). The signal was developed using ECL method and the antibody generated a specific band of CXCR4 at ~40 kDa position.

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - CXCR4 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Immunostaining of CXCR4 in human cervical carcinoma tissue sections.

Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - The A2AR & baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 & MYH11 expression levels in mouse PASMCs were analyzed by immunofluorescence staining (a; n = 3). CXCR4 protein is stained red, & MYH11 is stained green to indicate the PASMCs (×400; scale bars indicate 50 μm). CXCR4 fluorescence intensity was calculated (b; n = 3). CXCR4 protein expression levels in lung tissue were examined by western blot. GAPDH served as an internal control (c, d; n = 3). Data are presented as the mean ± SD. #Value significantly greater than the corresponding value in saline-treated normoxic mice (##P 50 CTCs/ 2.5 ml blood) was processed via ficoll density centrifugation & the PBMC fraction was collected. Immunomagnetic anti-CD45 depletion was performed on the obtained PBMCs & the remaining cells were washed, cytospunned onto the slides. Slides were stained for PSMA, EpCAM, sLex, & CXCR4 using the protocol as described in Figure S1. MDA, PC3, & KG1 cells were simultaneously stained as a control for the following markers: PSMA= Magenta, EpCAM= Yellow, HECA-452= Green, CXCR4= Red. All prostate CTCs expressed CXCR4, while, sLex expression was variable. The analysis of sLex intensity is shown in Figure 4. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24386459), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CXCR4 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to a C-terminal region of the human CXCR4 protein (within residues 300-352). [Swiss-Prot P61073]
• Localization: Cell membrane; Multi-pass membrane protein. Note: In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:5000
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33116799).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for CXCR4 Antibody - BSA Free

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediates hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (NB100-74396)(30)

Publications using NB100-74396

• Ranjith Palanisamy, Nimnaka Indrajith Kahingalage, David Archibald, Ilaria Casari, Marco Falasca Synergistic Anticancer Activity of Plumbagin and Xanthohumol Combination on Pancreatic Cancer Models. International journal of molecular sciences 2024-02-26 [PMID: 38397018]
• Wang Y, Liu Y, Li XY et al. Vagus nerve stimulation-induced stromal cell-derived factor-l alpha participates in angiogenesis and repair of infarcted hearts ESC heart failure 2023-08-29 [PMID: 37641543] (ICC/IF, Human)
• Filidou E, Kandilogiannakis L, Tarapatzi G et al. A Simplified and Effective Approach for the Isolation of Small Pluripotent Stem Cells Derived from Human Peripheral Blood Biomedicines 2023-03-05 [PMID: 36979766] (Immunocytochemistry/ Immunofluorescence, Human)
• Zhang S, Peng B, Chen Z et al. Brain-targeting, acid-responsive antioxidant nanoparticles for stroke treatment and drug delivery Bioactive Materials 2022-10-01 [PMID: 35386312] (Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Human, Rat)
• Tagami M, Kakehashi A, Katsuyama-Yoshikawa A et al. FOXP3 and CXCR4-positive regulatory T cells in the tumor stroma as indicators of tumor immunity in the conjunctival squamous cell carcinoma microenvironment PLOS ONE 2022-03-31 [PMID: 35358193] (IF/IHC, Human)
• Wu, H;Peng, B;Mohammed, FS;Gao, X;Qin, Z;Sheth, KN;Zhou, J;Jiang, Z; Brain Targeting, Antioxidant Polymeric Nanoparticles for Stroke Drug Delivery and Therapy Small (Weinheim an der Bergstrasse, Germany) [PMID: 35306743] (WB, Mouse)
• Zhang X, Detering L, Sultan D et al. C-X-C Chemokine Receptor Type 4-Targeted Imaging in Glioblastoma Multiforme Using 64Cu-Radiolabeled Ultrasmall Gold Nanoclusters ACS Applied Bio Materials 2021-12-23 [PMID: 35014818] (IHC-Fr)
• Guan W, Li F, Zhao Z et al. Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer Genes 2021-05-19 [PMID: 34069563] (WB, Mouse)
• Peng C, Chen XT, Xu H et al. Role of the CXCR4/ALK5/Smad3 Signaling Pathway in Cancer-Induced Bone Pain J Pain Res 2020-10-14 [PMID: 33116799] (WB, Rat)
• Zhang, S, Deng, G Et al. Autocatalytic Delivery of Brain Tumor-targeting, Size-shrinkable Nanoparticles for Treatment of Breast Cancer Brain Metastases. Adv Funct Mater 2020-04-03 [PMID: 32440263] (ELISA, Rat)
• Zhou Y, Zhang S, Chen Z et al. Targeted Delivery of Secretory Promelittin via Novel Poly(lactone co-beta amino ester) Nanoparticles for Treatment of Breast Cancer Brain Metastases Adv. Sci. 2020-01-19 [PMID: 32154067] (IF/IHC, FLOW, Human)
• D'Alterio C, Buoncervello M et al. Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1. J Exp Clin Cancer Res 2019-10-28 [PMID: 31661001] (IF/IHC, Mouse)
• Ma J, Zhang S, Liu J et al. Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells Small 2019-07-10 [PMID: 31290245]
• Heo GS, Zhao Y, Sultan D, Zhang X et al. Assessment of Copper Nanoclusters for Accurate in Vivo Tumor Imaging and Potential for Translation ACS Appl Mater Interfaces 2019-05-22 [PMID: 31074257] (WB)
• Serresi M, Siteur B, Hulsman D et al. Ezh2 inhibition in Kras-driven lung cancer amplifies inflammation and associated vulnerabilities J. Exp. Med. 2018-12-03 [PMID: 30487290] (IHC-P, Human)
• Huang X, Wu P, Huang F et al. Baicalin attenuates chronic hypoxia-induced pulmonary hypertension via adenosine A2A receptor-induced SDF-1/CXCR4/PI3K/AKT signaling J. Biomed. Sci. 2017-08-03 [PMID: 28774332] (ICC/IF, Mouse)
• Xie C, Gao X, Luo Y et al. Electroacupuncture modulates stromal cell-derived factor-1a expression and mobilization of bone marrow endothelial progenitor cells in focal cerebral ischemia/reperfusion model rats Brain Res. 2016-07-21 [PMID: 27453543] (WB, Human)
• Giusti I, Delle Monache S, Di Francesco M et al. From glioblastoma to endothelial cells through extracellular vesicles: messages for angiogenesis. Tumour Biol 2016-09-01 [PMID: 27448307] (Human)
• Mayorga M, Kiedrowski M, Shamhart P et al. Early up-regulation of myocardial CXCR4 expression is critical for Dimethyloxalylglycine (DMOG) induced cardiac improvement in acute myocardial infarction (MI). Am. J. Physiol. Heart Circ. Physiol. 2015-10-30 [PMID: 26519029] (IHC-P, Mouse)
• Lee HW, Cho HJ, Lee SJ et al. Tpl2 induces castration resistant prostate cancer progression and metastasis. Int. J. Cancer. 2014-10-01 [PMID: 25274482] (ICC/IF, WB, IHC-P, Human)
  
  Details:
  CXCR4 antibody used for WB and ICC-IF on PC3 and 22Rv1 cells in relation to Tpl2 (i.e. cells with Tpl2 knockdown and with Tpl2 over-expression) . Antibody also used in IHC-P for the detection of CXCR4 on orthotopic xenograft tumors (Figure 6).
• Gakhar G, Navarro VN, Jurish M et al. Circulating Tumor Cells from Prostate Cancer Patients Interact with E-Selectin under Physiologic Blood Flow. PLoS One 2013-12-27 [PMID: 24386459] (ICC/IF, Human)
• Hoh BL, Hosaka K, Downes DP et al. Stromal cell-derived factor-1 promoted angiogenesis and inflammatory cell infiltration in aneurysm walls. J Neurosurg. 2013-10-25 [PMID: 24160472]
• Huang Yf, Chen MJ, Wu MH, Hung SC. The use of hypoxic cultured mesenchymal stem cell for oncolytic virus therapy. Cancer Gene Ther 2013-04-26 [PMID: 23618949]
• Mo W, Chen J, Patel A et al. CXCR4/CXCL12 Mediate Autocrine Cell- Cycle Progression in NF1-Associated Malignant Peripheral Nerve Sheath Tumors. Cell 2013-02-28 [PMID: 23434321] (IHC-P, WB, Mouse)
• Chen C, Cao J, Song X et al. Adrenaline administration promotes the efficiency of granulocyte colony stimulating factor-mediated hematopoietic stem and progenitor cell mobilization in mice Int J Hematol 2012-12-08 [PMID: 23224606] (WB, Mouse)
• Kumagai K, Takeuchi R, Ishikawa H et al. Low-intensity pulsed ultrasound accelerates fracture healing by stimulation of recruitment of both local and circulating osteogenic progenitors J Orthop Res 2012-09-01 [PMID: 22419401] (ICC/IF, IF/IHC, Mouse)
• Agarwal U, Ghalayini W, Dong F et al. Role of cardiac myocyte CXCR4 expression in development and left ventricular remodeling after acute myocardial infarction. Circ Res 2010-09-03 [PMID: 20634485] (Mouse)
• Hasegawa T, McLeod DS, Prow T, Merges C, Grebe R, Lutty GA. Vascular Precursors in Developing Human Retina. Invest Ophthalmol Vis Sci;49(5):2178-92. 2008-05-01 [PMID: 18436851] (IF/IHC, Human)
• Stumm RK, Zhou C, Ara T et al. CXCR4 regulates interneuron migration in the developing neocortex. J Neurosci;23(12):5123-30. 2003-06-15 [PMID: 12832536] (IF/IHC, Rat)
• Stumm RK, Rummel J, Junker V et al. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci;22(14):5865-78. 2002-07-15 [PMID: 12122049] (WB, IF/IHC, ICC/IF, Rat, Human)

Review for CXCR4 Antibody (NB100-74396) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/11/2014

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human

FAQs for CXCR4 Antibody (NB100-74396). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 7852(Human)
  • 12767(Mouse)
• Uniprot:
  • P70658()