CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

CXCR7/RDC‑1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 11G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CXCR7/RDC-1 Antibody (11G8) [Unconjugated] Summary

• Immunogen: Human CXCR7 encoding plasmid
  Accession # P25106
• Specificity: Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCR7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

• ACKR3
• chemokine (C-X-C motif) receptor 7
• Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
• CMKOR1
• CXCR7
• CXC-R7
• CXCR-7
• G protein-coupled receptor
• GPR159
• GPR159G-protein coupled receptor 159
• RDC-1
• RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (MAB42273)(24)

Publications using MAB42273

• C D'Alterio, A Giardino, G Scognamigl, G Butturini, L Portella, G Guardascio, I Frigerio, M Montella, S Gobbo, G Martignoni, V Napolitano, F De Vita, F Tatangelo, R Franco, S Scala CXCR4-CXCL12-CXCR7 and PD-1/PD-L1 in Pancreatic Cancer: CXCL12 Predicts Survival of Radically Resected Patients Cells, 2022;11(21):. 2022 [PMID: 36359736] (IHC, Human)
• C D'Alterio, A Spina, L Arenare, P Chiodini, M Napolitano, F Galdiero, L Portella, V Simeon, S Signoriell, F Raspaglies, D Lorusso, C Pisano, N Colombo, GF Zannoni, NS Losito, R De Cecio, G Scognamigl, D Califano, D Russo, V Tuninetti, MC Piccirillo, P Gargiulo, F Perrone, S Pignata, S Scala Biological Role of Tumor/Stromal CXCR4-CXCL12-CXCR7 in MITO16A/MaNGO-OV2 Advanced Ovarian Cancer Patients Cancers, 2022;14(7):. 2022 [PMID: 35406620] (IHC, Human)
• SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022;23(2):. 2022 [PMID: 35055054] (Neutralization, Human)
• N Pluchino, R Mamillapal, S Shaikh, S Habata, A Tal, M Gaye, HS Taylor CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking J. Cell. Mol. Med., 2020;24(4):2464-2474. 2020 [PMID: 31904910] (IHC-P, Human)
• C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019;10(8):562. 2019 [PMID: 31332163] (Neutralization, Mouse)
• S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer Cancer Res., 2019;0(0):. 2019 [PMID: 30952632] (Flow Cytometry, Human)
• S Xu, J Tang, C Wang, J Liu, Y Fu, Y Luo CXCR7 promotes melanoma tumorigenesis via Src kinase signaling Cell Death Dis, 2019;10(3):191. 2019 [PMID: 30804329] (IHC, Human)
• M Wang, X Yang, M Wei, Z Wang The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer J Cancer, 2018;9(21):3898-3903. 2018 [PMID: 30410593] (IHC, Human)
• E Willems, M Dedobbelee, M Digregorio, A Lombard, N Goffart, PN Lumapat, J Lambert, P Van den Ac, M Szpakowska, A Chevigné, F Scholtes, B Rogister Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration Oncogene, 2018;0(0):. 2018 [PMID: 30082913] (Cell Selection, Human)
• N Pluchino, R Mamillapal, I Moridi, R Tal, HS Taylor G-Protein-Coupled Receptor CXCR7 Is Overexpressed in Human and Murine Endometriosis Reprod Sci, 2018;0(0):1933719118766. 2018 [PMID: 29587613] (IHC-P, Human)
• T D'huys, S Claes, T Van Loy, D Schols CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells Heliyon, 2018;4(3):e00557. 2018 [PMID: 29560468] (Flow Cytometry, Human)
• N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29180449] (Flow Cytometry, Human)
• YH Cheng, JM Eby, HM LaPorte, BF Volkman, M Majetschak Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function PLoS ONE, 2017;12(11):e0187949. 2017 [PMID: 29125867] (Functional Assay, Human)
• M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells Sci Rep, 2017;7(1):8289. 2017 [PMID: 28811579] (Bioassay, Human)
• Vashendriya V V Hira Cathepsin K cleavage of SDF-1? inhibits its chemotactic activity towards glioblastoma stem-like cells Biochim. Biophys. Acta, 2016;0(0):. 2016 [PMID: 28040478] (Flow Cytometry, Human)
• Muhlethaler-Mottet A, Liberman J, Ascencao K, Flahaut M, Balmas Bourloud K, Yan P, Jauquier N, Gross N, Joseph J The CXCR4/CXCR7/CXCL12 Axis Is Involved in a Secondary but Complex Control of Neuroblastoma Metastatic Cell Homing. PLoS ONE, 2015;10(5):e0125616. 2015 [PMID: 25955316] (Flow Cytometry, Human)
• Walsh T, Harper M, Poole A SDF-1alpha is a novel autocrine activator of platelets operating through its receptor CXCR4. Cell Signal, 2015;27(1):37-46. 2015 [PMID: 25283599] (Neutralization, Human)
• Liu Y, Carson-Walter E, Walter K Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells. PLoS ONE, 2014;9(8):e103938. 2014 [PMID: 25084358] (ICC, Human)
• Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration. BMC Cancer, 2014;14(0):407. 2014 [PMID: 24906407] (Western Blot, Human)
• Walters , M J, Ebsworth , K, Berahovich , R D, Penfold , M E T, Liu , S-C, Al Omran , R, Kioi , M, Chernikova , S B, Tseng , D, Mulkearns-Hubert , E E, Sinyuk , M, Ransohoff , R M, Lathia , J D, Karamchandani , J, Kohrt , H E K, Zhang , P, Powers , J P, Jaen , J C, Schall , T J, Merchant , M, Recht , L, Brown , J M Inhibition of CXCR7 extends survival following irradiation of brain tumours in mice and rats. Br J Cancer, 2014;110(5):1179-88. 2014 [PMID: 24423923] (IHC-P, Human)
• Ding B, Cao Z, Lis R, Nolan D, Guo P, Simons M, Penfold M, Shido K, Rabbany S, Rafii S Divergent angiocrine signals from vascular niche balance liver regeneration and fibrosis. Nature, 2014;505(7481):97-102. 2014 [PMID: 24256728] (Immunoprecipitation, Human)
• Liberman J, Sartelet H, Flahaut M, Muhlethaler-Mottet A, Coulon A, Nyalendo C, Vassal G, Joseph J, Gross N Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma. PLoS ONE, 2012;7(8):e43665. 2012 [PMID: 22916293] (ICC, Human)
• Canals M, Scholten DJ, de Munnik S, Han MK, Smit MJ, Leurs R Ubiquitination of CXCR7 controls receptor trafficking. PLoS ONE, 2012;7(3):e34192. 2012 [PMID: 22457824] (Bioassay, Human)
• Naumann U, Cameroni E, Pruenster M, Mahabaleshwar H, Raz E, Zerwes HG, Rot A, Thelen M CXCR7 functions as a scavenger for CXCL12 and CXCL11. PLoS ONE, 2010;5(2):e9175. 2010 [PMID: 20161793] (ICC, Human)

Reviews for CXCR7/RDC-1 Antibody (MAB42273) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 03/11/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: YQU0218091

reviewed by: cynthia morimoto ELISA Human 02/20/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 22457824
• Species: Human

Bioinformatics

• Gene Symbol: CXCR7
• Uniprot:
  • P25106()