CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

Images

 
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more
CXCR7/RDC‑1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at ...read more
CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. ...read more
CXCL12 induces CXCR4/CXCR7 and cell polarization in HUVECs. HUVECs were cultured on plastic Petri dishes (pretreated with PDL) with BSA stripes (the control group) and BSA plus CXCL12 stripes (CXCL12 group) for 5 min. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
11G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CXCR7/RDC-1 Antibody (11G8) [Unconjugated] Summary

Immunogen
Human CXCR7 encoding plasmid
Accession # P25106
Specificity
Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCR7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
MAB42273 in the following applications:

Publications
Read Publications using
MAB42273 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR7/RDC-1 Antibody (MAB42273)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 11 applications: Bioassay, Cell Selection, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Immunoprecipitation, Inhibition, Neutralization, Western Blot.


Filter By Application
Bioassay
(2)
Cell Selection
(1)
Flow Cytometry
(8)
Functional Assay
(1)
ICC
(5)
IHC
(6)
IHC-P
(3)
Immunoprecipitation
(1)
Inhibition
(1)
Neutralization
(4)
Western Blot
(2)
All Applications
Filter By Species
Human
(25)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using MAB42273 Applications Species
Hamshaw, I;Ellahouny, Y;Malusickis, A;Newman, L;Ortiz-Jacobs, D;Mueller, A; The role of PKC and PKD in CXCL12 and CXCL13 directed malignant melanoma and acute monocytic leukemic cancer cell migration Cellular signalling 2023-11-08 [PMID: 37949381] (Flow Cytometry, Human) Flow Cytometry Human
C D'Alterio, A Giardino, G Scognamigl, G Butturini, L Portella, G Guardascio, I Frigerio, M Montella, S Gobbo, G Martignoni, V Napolitano, F De Vita, F Tatangelo, R Franco, S Scala CXCR4-CXCL12-CXCR7 and PD-1/PD-L1 in Pancreatic Cancer: CXCL12 Predicts Survival of Radically Resected Patients Cells, 2022-10-22;11(21):. 2022-10-22 [PMID: 36359736] (IHC, Human) IHC Human
Brittany E. Hopkins, Ikuo Masuho, Dongjun Ren, Iredia D. Iyamu, Wei Lv, Neha Malik et al. Effects of Small Molecule Ligands on ACKR3 Receptors Molecular Pharmacology 9/1/2022 [PMID: 35809897]
C D'Alterio, A Spina, L Arenare, P Chiodini, M Napolitano, F Galdiero, L Portella, V Simeon, S Signoriell, F Raspaglies, D Lorusso, C Pisano, N Colombo, GF Zannoni, NS Losito, R De Cecio, G Scognamigl, D Califano, D Russo, V Tuninetti, MC Piccirillo, P Gargiulo, F Perrone, S Pignata, S Scala Biological Role of Tumor/Stromal CXCR4-CXCL12-CXCR7 in MITO16A/MaNGO-OV2 Advanced Ovarian Cancer Patients Cancers, 2022-04-06;14(7):. 2022-04-06 [PMID: 35406620] (IHC, Human) IHC Human
SF Mause, E Ritzel, A Deck, F Vogt, EA Liehn Engagement of the CXCL12-CXCR4 Axis in the Interaction of Endothelial Progenitor Cell and Smooth Muscle Cell to Promote Phenotype Control and Guard Vascular Homeostasis International Journal of Molecular Sciences, 2022-01-14;23(2):. 2022-01-14 [PMID: 35055054] (Neutralization, Human) Neutralization Human
M Meyrath, M Szpakowska, J Zeiner, L Massotte, MP Merz, T Benkel, K Simon, J Ohnmacht, JD Turner, R Krüger, V Seutin, M Ollert, E Kostenis, A Chevigné The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides Nat Commun, 2020-06-19;11(1):3033. 2020-06-19 [PMID: 32561830] (Flow Cytometry, Human) Flow Cytometry Human
ST Wei, YC Huang, ML Hsieh, YJ Lin, WC Shyu, HC Chen, CH Hsieh Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling Cell Death Dis, 2020-05-04;11(5):307. 5/4/2020 [PMID: 32366833]
N Pluchino, R Mamillapal, S Shaikh, S Habata, A Tal, M Gaye, HS Taylor CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking J. Cell. Mol. Med., 2020-01-06;24(4):2464-2474. 2020-01-06 [PMID: 31904910] (IHC-P, Human) IHC-P Human
C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019-07-22;10(8):562. 2019-07-22 [PMID: 31332163] (Neutralization, Mouse) Neutralization Mouse
Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al. Development and application of a high-content virion display human GPCR array Nature Communications 4/30/2019 [PMID: 31040288]
Show All 36 Publications.

Reviews for CXCR7/RDC-1 Antibody (MAB42273) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB42273:
Filter by Applications
IHC-Fr
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CXCR7/RDC-1 MAB42273
Enlarge
4
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 03/11/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotYQU0218091
  4
reviewed by:
Verified Customer
ELISA Human 02/20/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22457824
SpeciesHuman

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FAQs for CXCR7/RDC-1 Antibody (MAB42273) (0)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Kirsten Hattermann-Koch
03/11/2019
Application: IHC-Fr
Species: Mouse

 
Verified Customer
02/20/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CXCR7
Uniprot