CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

Images

 
CXCR7/RDC‑1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273) at 5 µg/mL for 1 hour at ...read more
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human CXCR7/RDC‑1 Monoclonal Antibody (Catalog # MAB42273, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
11G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CXCR7/RDC-1 Antibody (11G8) [Unconjugated] Summary

Immunogen
Human CXCR7 encoding plasmid
Accession # P25106
Specificity
Detects human CXCR7/RDC-1 in flow cytometry of five distinct human CXCR7 transfectants, but not their respective parental lines.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CXCR7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4
using
MAB42273 in the following applications:

Publications
Read Publications using
MAB42273 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR7/RDC-1 Antibody (11G8) [Unconjugated]

  • ACKR3
  • chemokine (C-X-C motif) receptor 7
  • Chemokine orphan receptor 1CMKOR1C-X-C chemokine receptor type 7
  • CMKOR1
  • CXCR7
  • CXC-R7
  • CXCR-7
  • G protein-coupled receptor
  • GPR159
  • GPR159G-protein coupled receptor 159
  • RDC-1
  • RDC1G-protein coupled receptor RDC1 homolog

Background

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I‑TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2, and 3, human and mouse CXCR7 share 84%, 100%, 96%, and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression (as assessed using anti-CXCR7, clone 9C4) (J. Biol. Chem. 2005, 280(42):35760, J. Immunol. 2006, 176(4):2197) indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (as assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells, and few other cell types (J. Exp. Med. 2006, 203(9):2201). The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR7/RDC-1 Antibody (MAB42273)(20)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 10 applications: Bioassay, Cell Selection, Flow Cytometry, Functional Assay, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Bioassay
(2)
Cell Selection
(1)
Flow Cytometry
(5)
Functional Assay
(1)
ICC
(3)
IHC
(2)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(19)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using MAB42273 Applications Species
C Ieranò, C D'Alterio, S Giarra, M Napolitano, G Rea, L Portella, A Santagata, AM Trotta, A Barbieri, V Campani, A Luciano, C Arra, AM Anniciello, G Botti, L Mayol, G De Rosa, R Pacelli, S Scala CXCL12 loaded-dermal filler captures CXCR4 expressing melanoma circulating tumor cells Cell Death Dis, 2019;10(8):562. 2019 [PMID: 31332163] (Neutralization, Mouse) Neutralization Mouse
S Li, KW Fong, G Gritsina, A Zhang, JC Zhao, J Kim, A Sharp, W Yuan, C Aversa, XJ Yang, PS Nelson, FY Feng, AM Chinnaiyan, JS de Bono, C Morrissey, MB Rettig, J Yu Activation of MAPK signaling by CXCR7 leads to enzalutamide resistance in prostate cancer Cancer Res., 2019;0(0):. 2019 [PMID: 30952632] (Flow Cytometry, Human) Flow Cytometry Human
S Xu, J Tang, C Wang, J Liu, Y Fu, Y Luo CXCR7 promotes melanoma tumorigenesis via Src kinase signaling Cell Death Dis, 2019;10(3):191. 2019 [PMID: 30804329] (IHC, Human) IHC Human
M Wang, X Yang, M Wei, Z Wang The Role of CXCL12 Axis in Lung Metastasis of Colorectal Cancer J Cancer, 2018;9(21):3898-3903. 2018 [PMID: 30410593] (IHC, Human) IHC Human
E Willems, M Dedobbelee, M Digregorio, A Lombard, N Goffart, PN Lumapat, J Lambert, P Van den Ac, M Szpakowska, A Chevigné, F Scholtes, B Rogister Aurora A plays a dual role in migration and survival of human glioblastoma cells according to the CXCL12 concentration Oncogene, 2018;0(0):. 2018 [PMID: 30082913] (Cell Selection, Human) Cell Selection Human
N Pluchino, R Mamillapal, I Moridi, R Tal, HS Taylor G-Protein-Coupled Receptor CXCR7 Is Overexpressed in Human and Murine Endometriosis Reprod Sci, 2018;0(0):1933719118766. 2018 [PMID: 29587613] (IHC-P, Human) IHC-P Human
T D'huys, S Claes, T Van Loy, D Schols CXCR7/ACKR3-targeting ligands interfere with X7 HIV-1 and HIV-2 entry and replication in human host cells Heliyon, 2018;4(3):e00557. 2018 [PMID: 29560468] (Flow Cytometry, Human) Flow Cytometry Human
N Montpas, G St-Onge, N Nama, D Rhainds, B Benredjem, M Girard, G Hickson, V Pons, N Heveker Ligand-specific conformational transitions and intracellular transport required for atypical chemokine receptor 3-mediated chemokine scavenging J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29180449] (Flow Cytometry, Human) Flow Cytometry Human
YH Cheng, JM Eby, HM LaPorte, BF Volkman, M Majetschak Effects of cognate, non-cognate and synthetic CXCR4 and ACKR3 ligands on human lung endothelial cell barrier function PLoS ONE, 2017;12(11):e0187949. 2017 [PMID: 29125867] (Functional Assay, Human) Functional Assay Human
M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells Sci Rep, 2017;7(1):8289. 2017 [PMID: 28811579] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

Reviews for CXCR7/RDC-1 Antibody (MAB42273) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB42273:
Filter by Applications
IHC-Fr
(1)
ELISA
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CXCR7/RDC-1 MAB42273
Enlarge
4
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 03/11/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotYQU0218091
  4
reviewed by:
Anonymous
ELISA Human 02/20/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 22457824
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22457824

Details

Detection NotesSee PMID 22457824

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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FAQs for CXCR7/RDC-1 Antibody (MAB42273) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB42273U
Alexa Fluor 405 FAB42273V
Alexa Fluor 488 FAB42273G
Alexa Fluor 594 FAB42273T
Alexa Fluor 647 FAB42273R
Alexa Fluor 700 FAB42273N
Alexa Fluor 750 FAB42273S
Allophycocyanin FAB4227A
Allophycocyanin FAB4227A
Phycoerythrin FAB4227P
Phycoerythrin FAB4227P

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Kirsten Hattermann-Koch
03/11/2019
Application: IHC-Fr
Species: Mouse

 
Anonymous
02/20/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol CXCR7
Uniprot