CXCR4 Antibody

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 protein in human small intestine tissue lysate using 1:500 dilution of rabbit polyclonal CXCR4 antibody (Lot A2). The signal was developed using ECL method and the antibody generated a specific band of CXCR4 at ~40 kDa position.

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-74396] - CXCR4 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Immunostaining of CXCR4 in human cervical carcinoma tissue sections.

Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CXCR4 Antibody Summary

• Immunogen: A synthetic peptide made to a C-terminal region of the human CXCR4 protein (within residues 300-352). [Swiss-Prot P61073]
• Localization: Cell membrane; Multi-pass membrane protein. Note: In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
• Application Notes: This CXCR4 antibody is useful for IHC-P sections, ICC/IF and WB. In WB a band is observed ~ 40 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24160472).
• Control:

  HeLa Whole Cell Lysate
  CXCR4 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for CXCR4 Antibody

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediates hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (NB100-74396)(18)

Publications using NB100-74396

• Ma J, Zhang S, Liu J et al. Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells Small Jul 10 2019 [PMID: 31290245]
• Heo GS, Zhao Y, Sultan D, Zhang X et al. Assessment of Copper Nanoclusters for Accurate in Vivo Tumor Imaging and Potential for Translation ACS Appl Mater Interfaces May 22 2019 [PMID: 31074257] (WB)
• Serresi M, Siteur B, Hulsman D et al. Ezh2 inhibition in Kras-driven lung cancer amplifies inflammation and associated vulnerabilities J. Exp. Med. Dec 3 2018 [PMID: 30487290] (IHC-P, Human)
• Huang X, Wu P, Huang F et al. Baicalin attenuates chronic hypoxia-induced pulmonary hypertension via adenosine A2A receptor-induced SDF-1/CXCR4/PI3K/AKT signaling J. Biomed. Sci. 2017 Aug 03 [PMID: 28774332] (ICC/IF, Mouse)
• Xie C, Gao X, Luo Y et al. Electroacupuncture modulates stromal cell-derived factor-1a expression and mobilization of bone marrow endothelial progenitor cells in focal cerebral ischemia/reperfusion model rats Brain Res. 2016 Jul 21 [PMID: 27453543] (WB, Human)
• Giusti I, Delle Monache S, Di Francesco M et al. From glioblastoma to endothelial cells through extracellular vesicles: messages for angiogenesis. Tumour Biol 2016 Sep [PMID: 27448307] (Human)
• Mayorga M, Kiedrowski M, Shamhart P et al. Early up-regulation of myocardial CXCR4 expression is critical for Dimethyloxalylglycine (DMOG) induced cardiac improvement in acute myocardial infarction (MI). Am. J. Physiol. Heart Circ. Physiol. 2015 Oct 30 [PMID: 26519029] (IHC-P, Mouse)
• Lee HW, Cho HJ, Lee SJ et al. Tpl2 induces castration resistant prostate cancer progression and metastasis. Int. J. Cancer. 2014 Oct 01 [PMID: 25274482] (WB, ICC/IF, IHC-P, Human)
  
  Details:
  CXCR4 antibody used for WB and ICC-IF on PC3 and 22Rv1 cells in relation to Tpl2 (i.e. cells with Tpl2 knockdown and with Tpl2 over-expression). Antibody also used in IHC-P for the detection of CXCR4 on orthotopic xenograft tumors (Figure 6).
• Gakhar G, Navarro VN, Jurish M et al. Circulating Tumor Cells from Prostate Cancer Patients Interact with E-Selectin under Physiologic Blood Flow. PLoS One 2013 Dec 27 [PMID: 24386459] (ICC/IF, Human)
• Hoh BL, Hosaka K, Downes DP et al. Stromal cell-derived factor-1 promoted angiogenesis and inflammatory cell infiltration in aneurysm walls. J Neurosurg. 2013 Oct 25 [PMID: 24160472]
• Huang Yf, Chen MJ, Wu MH, Hung SC. The use of hypoxic cultured mesenchymal stem cell for oncolytic virus therapy. Cancer Gene Ther 2013 Apr 26 [PMID: 23618949]
• Mo W, Chen J, Patel A et al. CXCR4/CXCL12 Mediate Autocrine Cell- Cycle Progression in NF1-Associated Malignant Peripheral Nerve Sheath Tumors. Cell 2013 Feb 28 [PMID: 23434321] (WB, IHC-P, Mouse)
• Chen C, Cao J, Song X et al. Adrenaline administration promotes the efficiency of granulocyte colony stimulating factor-mediated hematopoietic stem and progenitor cell mobilization in mice Int J Hematol 2012 Dec 8 [PMID: 23224606] (WB, Mouse)
• Kumagai K, Takeuchi R, Ishikawa H et al. Low-intensity pulsed ultrasound accelerates fracture healing by stimulation of recruitment of both local and circulating osteogenic progenitors J Orthop Res 2012 Sep [PMID: 22419401] (IHC, ICC/IF, Mouse)
• Agarwal U, Ghalayini W, Dong F et al. Role of cardiac myocyte CXCR4 expression in development and left ventricular remodeling after acute myocardial infarction. Circ Res 2010 Sep 3 [PMID: 20634485] (Mouse)
• Hasegawa T, McLeod DS, Prow T, Merges C, Grebe R, Lutty GA. Vascular Precursors in Developing Human Retina. Invest Ophthalmol Vis Sci;49(5):2178-92. May 1 2008. [PMID: 18436851] (IHC, Human)
• Stumm RK, Zhou C, Ara T et al. CXCR4 regulates interneuron migration in the developing neocortex. J Neurosci;23(12):5123-30. 2003 Jun 15. [PMID: 12832536] (IHC, Rat)
• Stumm RK, Rummel J, Junker V et al. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci;22(14):5865-78. 2002 Jul 15. [PMID: 12122049] (IHC, ICC/IF, WB, Human, Rat)

Review for CXCR4 Antibody (NB100-74396) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 09/11/2014

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human
• Comments: The antibody gives multiple bands but the one at 41KDa is a stronger one.

Blocking

• Blocking Details: 5% BSA in PBS

Primary Anitbody

• Dilution Ratio: 1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

• Secondary Description: anti-rabbit IgG HRP conjugate (Jackson Labs)
• Secondary Manufacturer Cat#: 111-005-045
• Secondary Concentration: 1:20000

Details

• Detection Notes: Probed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Comments

• Comments: The antibody gives multiple bands but the one at 41KDa is a stronger one.

FAQs for CXCR4 Antibody (NB100-74396). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 7852(Human)
  • 12767(Mouse)
• Uniprot:
  • P70658()