CXCR4 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - Optimization of immunofluorescence staining of CXCR4 protein. MDA, PC3, KG1 cells were used for the optimization experiments. Cells were seeded ...read more
Biological Strategies: Western Blot: CXCR4 Antibody [NB100-74396] - The A2AR and baicalin attenuated CXCR4 expression in the hypoxia-induced PAH mouse model. CXCR4 protein expression levels in lung tissue were ...read more
Biological Strategies: Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R54 in combination with nivolumab reduced the expression of CXCR4-CXCL12 and PD-L1 in PES43 tumors. Representative IHC pictures ...read more
Biological Strategies: Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Pep R in combination with anti-PD-1 reduced the expression of CXCR4-CXCL12 and PD-L1 in MC38 tumors. Representative IHC pictures for ...read more
Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 in HeLa whole cell extract.
Western Blot: CXCR4 Antibody [NB100-74396] - Analysis of CXCR4 protein in human small intestine tissue lysate using 1:500 dilution of rabbit polyclonal CXCR4 antibody (Lot A2). The signal was developed using ECL method ...read more
Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody [NB100-74396] - CXCR4 antibody was tested in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: CXCR4 Antibody [NB100-74396] - Immunostaining of CXCR4 in human cervical carcinoma tissue sections.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
   

Biological Strategies

     

Order Details

CXCR4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of the human CXCR4 protein (within residues 300-352). [Swiss-Prot P61073]
Localization
Cell membrane; Multi-pass membrane protein. Note: In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CXCR4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:5000
Control
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NB100-74396 in the following applications:

Publications
Read Publications using
NB100-74396 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33116799).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for CXCR4 Antibody

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201E
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LAP-3
  • LCR1
  • LESTR
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3R
  • NPY3RWHIM
  • NPYR
  • NPYRL
  • NPYY3R
  • pCXCR4
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor
  • WHIMS

Background

CXCR4 (C-X-C chemokine receptor type 4) is a member of GPCR1 family and is expressed as a multipass membrane protein in several tissues wherein it acts as a receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. CXCR4 is involved in hematopoiesis, cardiac ventricular septum formation and plays an essential role in vascularization of the gastrointestinal tract via regulating vascular branching and/or remodeling processes in endothelial cells. CXCR4 is involved in cerebellar development and mediates hippocampal-neuron survival in CNS. It also acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates, and promotes Env-mediated fusion of the virus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR4 Antibody (NB100-74396)(24)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(1)
ICC/IF
(5)
IHC
(7)
IHC-P
(4)
WB
(9)
All Applications
Filter By Species
Human
(9)
Mouse
(8)
Rat
(3)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB100-74396 Applications Species
Guan W, Li F, Zhao Z et al. Tumor-Associated Macrophage Promotes the Survival of Cancer Cells upon Docetaxel Chemotherapy via the CSF1/CSF1R-CXCL12/CXCR4 Axis in Castration-Resistant Prostate Cancer Genes May 19 2021 [PMID: 34069563] (WB, Mouse) WB Mouse
Serresi M, Siteur B, Hulsman D et al. Ezh2 inhibition in Kras-driven lung cancer amplifies inflammation and associated vulnerabilities J. Exp. Med. Dec 3 2018 [PMID: 30487290] (IHC-P, Human) IHC-P Human
Mayorga M, Kiedrowski M, Shamhart P et al. Early up-regulation of myocardial CXCR4 expression is critical for Dimethyloxalylglycine (DMOG) induced cardiac improvement in acute myocardial infarction (MI). Am. J. Physiol. Heart Circ. Physiol. 2015 Oct 30 [PMID: 26519029] (IHC-P, Mouse) IHC-P Mouse
Tagami M, Kakehashi A, Katsuyama-Yoshikawa A et al. FOXP3/CD8 ratio and CXCR4-positive regulatory T cells as indicators of tumor immunity in the conjunctival squamous cell carcinoma microenvironment Research Square Mar 5 2021 (IHC, Human) IHC Human
Peng C, Chen XT, Xu H et al. Role of the CXCR4/ALK5/Smad3 Signaling Pathway in Cancer-Induced Bone Pain J Pain Res Oct 14 2020 [PMID: 33116799] (WB, Rat) WB Rat
Zhou Y, Zhang S, Chen Z et al. Targeted Delivery of Secretory Promelittin via Novel Poly(lactone co-beta amino ester) Nanoparticles for Treatment of Breast Cancer Brain Metastases Adv. Sci. Jan 19 2020 [PMID: 32154067] (Flow, IHC, Human) Flow, IHC Human
D'Alterio C, Buoncervello M et al. Targeting CXCR4 potentiates anti-PD-1 efficacy modifying the tumor microenvironment and inhibiting neoplastic PD-1. J Exp Clin Cancer Res Oct 28 2019 [PMID: 31661001] (IHC, Mouse) IHC Mouse
Ma J, Zhang S, Liu J et al. Targeted Drug Delivery to Stroke via Chemotactic Recruitment of Nanoparticles Coated with Membrane of Engineered Neural Stem Cells Small Jul 10 2019 [PMID: 31290245]
Heo GS, Zhao Y, Sultan D, Zhang X et al. Assessment of Copper Nanoclusters for Accurate in Vivo Tumor Imaging and Potential for Translation ACS Appl Mater Interfaces May 22 2019 [PMID: 31074257] (WB) WB
Huang X, Wu P, Huang F et al. Baicalin attenuates chronic hypoxia-induced pulmonary hypertension via adenosine A2A receptor-induced SDF-1/CXCR4/PI3K/AKT signaling J. Biomed. Sci. 2017 Aug 03 [PMID: 28774332] (ICC/IF, Mouse) ICC/IF Mouse
Show All 24 Publications.

Review for CXCR4 Antibody (NB100-74396) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-74396:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot CXCR4 NB100-74396
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3
reviewed by:
Verified Customer
WB Human 09/11/2014
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Summary

ApplicationWestern Blot
Sample Tested293T lysate
SpeciesHuman
CommentsThe antibody gives multiple bands but the one at 41KDa is a stronger one.

Blocking

Blocking Details5% BSA in PBS

Primary Anitbody

Dilution Ratio1:1000 diluted in PBS with triton X-100, blot was incubated at room temperature on a rocker

Secondary Antibody

Secondary Descriptionanti-rabbit IgG HRP conjugate (Jackson Labs)
Secondary Manufacturer Cat#111-005-045
Secondary Concentration1:20000

Details

Detection NotesProbed using Visualizer Western Blot Detection Kit (Millipore) and captured on X-ray films, 30 sec exposure

Comments

CommentsThe antibody gives multiple bands but the one at 41KDa is a stronger one.

Product General Protocols

View specific protocols for CXCR4 Antibody (NB100-74396): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CXCR4 Antibody (NB100-74396). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for CXCR4 Antibody (NB100-74396)

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Research Areas for CXCR4 Antibody (NB100-74396)

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Blogs on CXCR4.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.

CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.

Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.

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Recent Reviews

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Verified Customer
09/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CXCR4
Entrez
Uniprot