CTGF/CCN2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.01 mg/ml

CTGF/CCN2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of human CTGF (between residues 299-349). [UniProt# P29279]
Localization
Extracellular matrix and as a soluble form.
Predicted Species
Porcine (100%), Chicken (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTGF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunohistochemistry
Application Notes
This CTGF antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Kidney Lysate (NB820-59661)
Control Peptide
CTGF/CCN2 Peptide (NB100-724PEP)
Publications
Read Publications using
NB100-724 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Predicted to react with porcine, bovine, chicken and sheep based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.01 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CTGF/CCN2 Antibody

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

CTGF (connective tissue growth factor) is a cysteine rich extracellular matrix (ECM) protein that belongs to CCN family with other members namely cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells and is also expressed by cells from bone marrow, thymus, and Wilms tumors. CTGF expression is induced by BMP-2, TGF-beta1, glucocorticoids, retinoids, taurine, endothelin, cortisol in different cell types, however, TGF-beta1 has been suggested to be the most potent CTGF inducer regardless of cell type. CTGF has been shown to regulate a diverse array of cellular functions including proliferation, migration, adhesion, survival, differentiation and synthesis of ECM proteins in different type of cells. CTGF has also been involved in angiogenesis, chondrogenesis, osteogenesis, wound healing, fibrosis and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1640
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-356
Species: Hu, Rb
Applications: WB, Simple Western, B/N, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF4309
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP1-84037
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
NBP1-77836
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC

Publications for CTGF/CCN2 Antibody (NB100-724)(4)

Reviews for CTGF/CCN2 Antibody (NB100-724) (0)

There are no reviews for CTGF/CCN2 Antibody (NB100-724). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for CTGF/CCN2 Antibody (NB100-724): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CTGF/CCN2 Antibody (NB100-724). (Showing 1 - 2 of 2 FAQs).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
  2. Can this antibody (Catalog number NB100-724) be used for paraffin section?
    • The IHC application was added to the product datasheet for NB100-724 based on citation of the antibody in the following publication: NCBI Site The authors used paraffin-embedded tissue sections.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-724AF405
Alexa Fluor 488 Labeled NB100-724AF488
Alexa Fluor 647 Labeled NB100-724AF647
Alexa Fluor 700 Labeled NB100-724AF700
Biotin Labeled NB100-724B
DyLight 405 Labeled NB100-724V
DyLight 488 Labeled NB100-724G
DyLight 550 Labeled NB100-724R
DyLight 650 Labeled NB100-724C
FITC Labeled NB100-724F
HRP Labeled NB100-724H

Additional CTGF/CCN2 Products

Bioinformatics Tool for CTGF/CCN2 Antibody (NB100-724)

Discover related pathways, diseases and genes to CTGF/CCN2 Antibody (NB100-724). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CTGF/CCN2 Antibody (NB100-724)

Discover more about diseases related to CTGF/CCN2 Antibody (NB100-724).
 

Pathways for CTGF/CCN2 Antibody (NB100-724)

View related products by pathway.

PTMs for CTGF/CCN2 Antibody (NB100-724)

Learn more about PTMs related to CTGF/CCN2 Antibody (NB100-724).
 

Research Areas for CTGF/CCN2 Antibody (NB100-724)

Find related products by research area.

Blogs on CTGF/CCN2

There are no specific blogs for CTGF/CCN2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CTGF/CCN2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CTGF
Entrez
Uniprot