CTGF/CCN2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.01 mg/ml

CTGF/CCN2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of human CTGF (between residues 299-349). [UniProt# P29279]
Localization
Extracellular matrix and as a soluble form.
Predicted Species
Porcine (100%), Bovine (100%), Chicken (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTGF
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
In Western blot a band is observed at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28562206).
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
CTGF/CCN2 Knockout HeLa Cell Lysate
Control Peptide
Publications
Read Publications using
NB100-724 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.01 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CTGF/CCN2 Antibody

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

CTGF (connective tissue growth factor) is a cysteine rich extracellular matrix (ECM) protein that belongs to CCN family with other members namely cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells and is also expressed by cells from bone marrow, thymus, and Wilms tumors. CTGF expression is induced by BMP-2, TGF-beta1, glucocorticoids, retinoids, taurine, endothelin, cortisol in different cell types, however, TGF-beta1 has been suggested to be the most potent CTGF inducer regardless of cell type. CTGF has been shown to regulate a diverse array of cellular functions including proliferation, migration, adhesion, survival, differentiation and synthesis of ECM proteins in different type of cells. CTGF has also been involved in angiogenesis, chondrogenesis, osteogenesis, wound healing, fibrosis and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTGF/CCN2 Antibody (NB100-724)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
Rat
(2)
All Species

Reviews for CTGF/CCN2 Antibody (NB100-724) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CTGF/CCN2 Antibody (NB100-724). (Showing 1 - 2 of 2 FAQs).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
  2. Can this antibody (Catalog number NB100-724) be used for paraffin section?
    • The IHC application was added to the product datasheet for NB100-724 based on citation of the antibody in the following publication: NCBI Site The authors used paraffin-embedded tissue sections.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-724AF350
Alexa Fluor 405 NB100-724AF405
Alexa Fluor 488 NB100-724AF488
Alexa Fluor 532 NB100-724AF532
Alexa Fluor 594 NB100-724AF594
Alexa Fluor 647 NB100-724AF647
Alexa Fluor 700 NB100-724AF700
Alexa Fluor 750 NB100-724AF750
Biotin NB100-724B
DyLight 350 NB100-724UV
DyLight 405 NB100-724V
DyLight 488 NB100-724G
DyLight 550 NB100-724R
DyLight 594 NB100-724DL594
DyLight 650 NB100-724C
DyLight 680 NB100-724FR
DyLight 755 NB100-724IR
FITC NB100-724F
HRP NB100-724H
Janelia Fluor 549 NB100-724JF549
Janelia Fluor 646 NB100-724JF646

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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Bioinformatics

Gene Symbol CTGF
Entrez
Uniprot
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