Immunocytochemistry/ Immunofluorescence: CTGF Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Immunogen affinity purified
This CTGF antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed at ~38 kDa.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human, mouse and rat. Predicted to react with porcine, bovine, chicken and sheep based on 100% sequence homology.
Alternate Names for CTGF/CCN2 Antibody
connective tissue growth factor
Hypertrophic chondrocyte-specific protein 24
IGF-binding protein 8
IGFBP8CCN family member 2
Insulin-like growth factor-binding protein 8
CTGF (connective tissue growth factor) is a cysteine rich extracellular matrix (ECM) protein that belongs to CCN family with other members namely cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells and is also expressed by cells from bone marrow, thymus, and Wilms tumors. CTGF expression is induced by BMP-2, TGF-beta1, glucocorticoids, retinoids, taurine, endothelin, cortisol in different cell types, however, TGF-beta1 has been suggested to be the most potent CTGF inducer regardless of cell type. CTGF has been shown to regulate a diverse array of cellular functions including proliferation, migration, adhesion, survival, differentiation and synthesis of ECM proteins in different type of cells. CTGF has also been involved in angiogenesis, chondrogenesis, osteogenesis, wound healing, fibrosis and carcinogenesis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CTGF/CCN2 Antibody (NB100-724). (Showing 1 - 2 of 2 FAQs).
We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
Can this antibody (Catalog number NB100-724) be used for paraffin section?
The IHC application was added to the product datasheet for NB100-724 based on citation of the antibody in the following publication: NCBI Site The authors used paraffin-embedded tissue sections.