CTGF/CCN2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody [NB100-724] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of CTGF/CCN2 antibody (NB100-724). The staining was developed using HRP labeled ...read more
Overexpression of IRF5 in MCF-7 and MDA-MB-231 cells sensitizes them to DNA damage-induced growth inhibition. A. Endogenous IRF expression was analyzed by Western blot in transformed mammary epithelial cell lines. ...read more
Repressive chromatin induced on p21 promoter in TRF2 dependent way. (A–C) Loss of chromatin activation marks H3K4Me, H3K4Me2 in stable TRF2 expressed HT1080 cells (A); increase in LSD1 occupancy in TRF2 ...read more
Absence of Atm does not relieve the selective pressure to inactivate p53 during chemical carcinogenesis.A. Representative images of 3MC-fibrosarcomas immunostained for Arf, p53 and p21 (see also Table 1). The upper and ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CTGF/CCN2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of human CTGF (between residues 299-349). [UniProt# P29279]
Localization
Extracellular matrix and as a soluble form.
Predicted Species
Porcine (100%), Bovine (100%), Chicken (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin reported in scientific literature (PMID 28562206)
  • Western Blot 1:1000 - 1:2000
Application Notes
In Western blot a band is observed at ~38 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
CTGF/CCN2 Peptide (NB100-724PEP)
Publications
Read Publications using
NB100-724 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CTGF/CCN2 Antibody

  • CCN2
  • CCN2IGFBP-8
  • connective tissue growth factor
  • CTGF
  • CTGRP
  • Fisp12
  • HCS24
  • Hypertrophic chondrocyte-specific protein 24
  • IBP-8
  • IGF-binding protein 8
  • IGFBP-8
  • IGFBP8CCN family member 2
  • Insulin-like growth factor-binding protein 8
  • MGC102839
  • NOV2

Background

CTGF (connective tissue growth factor) is a cysteine rich extracellular matrix (ECM) protein that belongs to CCN family with other members namely cysteine-rich 61 (CYR61/CCN1), connective tIssue growth factor (CTGF/CCN2), nephroblastoma overexpressed (NOV/CCN3), and Wnt-induced secreted proteins-1 (WISP-1/CCN4), -2 (WISP-2/CCN5) and -3 (WISP-3/CCN6). CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells and is also expressed by cells from bone marrow, thymus, and Wilms tumors. CTGF expression is induced by BMP-2, TGF-beta1, glucocorticoids, retinoids, taurine, endothelin, cortisol in different cell types, however, TGF-beta1 has been suggested to be the most potent CTGF inducer regardless of cell type. CTGF has been shown to regulate a diverse array of cellular functions including proliferation, migration, adhesion, survival, differentiation and synthesis of ECM proteins in different type of cells. CTGF has also been involved in angiogenesis, chondrogenesis, osteogenesis, wound healing, fibrosis and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CTGF/CCN2 Antibody (NB100-724)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, RIP, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(2)
RIP
(1)
WB
(4)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-724 Applications Species
Buse J Einfluss einer ERK1/2-Resistenz auf Zellwachstum und Invasivit�t von triple-negativen Mammakarzinomzellen Thesis 2023-01-27
Huang, HC;Wang, TY;Rousseau, J;Mungaray, M;Michaud, C;Plaisier, C;Chen, ZB;Wang, KC; Lesion-specific suppression of YAP/TAZ by biomimetic nanodrug ameliorates atherosclerosis development bioRxiv : the preprint server for biology 2023-04-26 [PMID: 37163067] (WB, Mouse) WB Mouse
Hazra R, Brine L, Garcia L et al. Platr4 is an early embryonic lncRNA that exerts its function downstream on cardiogenic mesodermal lineage commitment Developmental cell 2022-11-07 [PMID: 36347239] (RIP, Mouse) RIP Mouse
Hellinger JW, SchOmel F, Buse JV et al. Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling Sci Rep 2020-10-21 [PMID: 33087801] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Wu SH, Lu IC, Tai MH et al. Erythropoietin Alleviates Burn-induced Muscle Wasting Int J Med Sci 2020-01-01 [PMID: 31929736] (WB, Rat) WB Rat
Ho LTY, Osterwald A, Ruf I et al. Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity Biochim Biophys Acta Mol Basis Dis 2019-10-21 [PMID: 31648019] (WB, Human) WB Human
Pan X, Jing Y, Liu W et al. Lipopolysaccharide induces the differentiation of hepatic progenitor cells into myofibroblasts via activation of the Hedgehog signalling pathway. Cell Cycle. 2017-05-31 [PMID: 28562206]
Zhu L, Zhao S, Liu S et al. PTEN Regulates Renal Extracellular Matrix Deposit via Increased CTGF in Diabetes Mellitus J Cell Biochem [PMID: 26447680]
Yijing L, Liu H, Yuan C et al. The effects of qindan-capsule-containing serum on the TGF-beta1/ERK signaling pathway, matrix metalloproteinase synthesis and cell function in adventitial fibroblasts. Pharm Biol 2013-02-04 [PMID: 23373709] (WB, Rat) WB Rat
Alfaro MP, Deskins DL, Wallus M et al. A physiological role for connective tissue growth factor in early wound healing Lab Invest 2012-11-19 [PMID: 23212098] (ICC/IF, IF/IHC, Mouse) ICC/IF, IF/IHC Mouse

Reviews for CTGF/CCN2 Antibody (NB100-724) (0)

There are no reviews for CTGF/CCN2 Antibody (NB100-724). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CTGF/CCN2 Antibody (NB100-724). (Showing 1 - 2 of 2 FAQs).

  1. We are looking for a pair of mouse CTGF antibody for ELISA, we prefer no azide and/or glycerol, and in carrier free form (no EDTA, no Tris, no BSA). For the validation, we need approximately 100ug. If it works, we'll order more later.
    • Unfortunately we do not carry any CTGF antibodies that suit your specifications. We do carry AbSelect antibody purification kits that will remove BSA, Tris and azide from antibody formulations which may be an option for you.
  2. Can this antibody (Catalog number NB100-724) be used for paraffin section?
    • The IHC application was added to the product datasheet for NB100-724 based on citation of the antibody in the following publication: NCBI Site The authors used paraffin-embedded tissue sections.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CTGF/CCN2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol CCN2
Entrez
Uniprot