Smad3 Antibody

Images

 
Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a ...read more
Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.
Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Pm, Ch, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Smad3 Antibody Summary

Immunogen
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Predicted Species
Zebrafish (90%), Chicken (90%), Xenopus (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 0.5 - 2 ug/mL
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 5 Reviews rated 3.2
using
NB100-56479 in the following applications:

Publications
Read Publications using
NB100-56479 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Smad3 Antibody

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3

Background

SMAD3, a member of the SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. SMAD3 is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Publications for Smad3 Antibody (NB100-56479)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Canine.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
WB
(5)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Canine
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-56479 Applications Species
Chen B, Li R, Hernandez S et al. Differential effects of Smad2 and Smad3 in regulation of macrophage phenotype and function in the infarcted myocardium Journal of Molecular and Cellular Cardiology 2022-07-01 [PMID: 35780861]
Smith SS, Chu D, Qu T et al. Species-specific sensitivity to TGF? signaling and changes to the Mmp13 promoter underlie avian jaw development and evolution eLife 2022-06-06 [PMID: 35666955] (WB) WB
Wu X, Wang H, Chen H et al. Overexpression of smad7 inhibits the TGF-beta/Smad signaling pathway and EMT in NPHP1-defective MDCK cells Biochem Biophys Res Commun 2021-12-10 [PMID: 34689106] (WB, ICC/IF, Canine) WB, ICC/IF Canine
Li Y, Zhao S, Xu S et al. Palmitoylethanolamide (PEA) reduces postoperative adhesions after experimental strabismus surgery in rabbits by suppressing canonical and non-canonical TGF beta signaling through PPAR alpha Biochemical pharmacology 2020-12-29 [PMID: 33385371] (WB, Mouse) WB Mouse
Chen SM, Hsu LJ, Lee HL et al. Lactobacillus Attenuate the Progression of Pancreatic Cancer Promoted by Porphyromonas Gingivalis in K-rasG12D Transgenic Mice Cancers (Basel) 2020-11-26 [PMID: 33255941] (IHC-P, Mouse)

Details:
Pancreas samples from transgenic mice exposed to P. gingivalis underwent immunohistochemical analysis.
IHC-P Mouse
Sivadas VP, George NA, Kattoor J, Kannan S. Novel mutations and expression alterations in SMAD3/TGFBR2 genes in oral carcinoma correlate with poor prognosis. Genes Chromosomes Cancer 2013-11-01 [PMID: 23913824] (WB, Human) WB Human
Ruebel KH, Leontovich AA, Tanizaki Y et al. Effects of TGFbeta1 on gene expression in the HP75 human pituitary tumor cell line identified by gene expression profiling. Endocrine. 2008-02-01 [PMID: 18401765] (WB, Human) WB Human

Reviews for Smad3 Antibody (NB100-56479) (5) 3.25

Average Rating: 3.2
(Based on 5 reviews)
We have 5 reviews tested in 4 species: Human, Mouse, Bovine, mice.

Reviews using NB100-56479:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
Bovine
(1)
mice
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Smad3 NB100-56479
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5
reviewed by:
Verified Customer
WB Mouse 08/02/2021
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Summary

ApplicationWestern Blot
Sample TestedLung tissue
SpeciesMouse
Lot07172507B-3
Western Blot Smad3 NB100-56479
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1
reviewed by:
Verified Customer
WB mice 05/26/2021
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Summary

ApplicationWestern Blot
Sample Testedeye tissue
Speciesmice
Lot07172507B-4

Comments

Commentsused at 1:100 overnight.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Western Blot Smad3 NB100-56479
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2
reviewed by:
Verified Customer
WB Bovine 05/26/2021
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Summary

ApplicationWestern Blot
Sample Testedeye tissue
SpeciesBovine
Lot07172507B-4

Comments

CommentsUsed at 1:1000- for overnight.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
Western Blot Smad3 NB100-56479
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3
reviewed by:
Verified Customer
WB Human 05/24/2021
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Summary

ApplicationWestern Blot
Sample Testedocular tissue
SpeciesHuman
Lot07172507B-4

Comments

Commentsused at 1:1000 and incubated at 4degrees overnight
Western Blot Smad3 NB100-56479
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 06/17/2019
View

Summary

ApplicationWestern Blot
Sample TestedHuman fibroblast and HeLa whole cell lysate
SpeciesMouse
Lotimg-5848a

Comments

Comments1:1000 dilution
1 hour blocking in milk 5%

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Smad3 Antibody (NB100-56479). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Smad3 Products

Research Areas for Smad3 Antibody (NB100-56479)

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Blogs on Smad3.

TGF-beta for treating degenerative intervertebral disc disease
By Jamshed Arslan Pharm.D. Our upright posture and balance depend on a jelly-like material, called nucleus pulposus (NP), in the middle of intervertebral discs. NP cells protect us from disc degeneration by maintain...  Read full blog post.

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Recent Reviews

3.2
5
2
4
0
3
1
2
1
1
1

Verified Customer
08/02/2021
Application: WB
Species: Mouse

Verified Customer
05/26/2021
Application: WB
Species: mice

Verified Customer
05/26/2021
Application: WB
Species: Bovine

Bioinformatics

Gene Symbol SMAD3
Entrez
Uniprot