Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a ...read more
Immunocytochemistry/ Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.
Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified
Alternate Names for Smad3 Antibody
DKFZp586N0721
DKFZp686J10186
hMAD-3
hSMAD3
HsT17436
JV15-2MGC60396
MAD homolog 3
mad protein homolog
MAD, mothers against decapentaplegic homolog 3 (Drosophila)
MAD, mothers against decapentaplegic homolog 3
mad3
MADH3mad homolog JV15-2
mothers against decapentaplegic homolog 3
Mothers against DPP homolog 3
SMA- and MAD-related protein 3
SMAD 3
SMAD family member 3HSPC193
SMAD, mothers against DPP homolog 3 (Drosophila)
SMAD, mothers against DPP homolog 3
Smad3
Background
SMAD3, a member of the SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. SMAD3 is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Smad3 Antibody (NB100-56479). (Showing 1 - 1 of 1 FAQs).
I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.
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