Smad3 Antibody

Western Blot: Smad3 Antibody [NB100-56479] - Analysis in human fibroblast and HeLa whole cell lysates. Untreated lysates show a band around 55 kDa. 1:1000 dilution, 1 hour blocking in milk 5%. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: Smad3 Antibody [NB100-56479] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Smad3 at 10 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Smad3 Antibody [NB100-56479] - Human liver tissue probed with SMAD3 antibody (bottom left, right) and isotype control antibody (top left) at 10 ug/mL.

Western Blot: Smad3 Antibody [NB100-56479] - Analysis using SMAD3 antibody. Lysate from human liver in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver and 4) rat liver probed with SMAD3 antibody at 0.5 ug/mL. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Pm, Ch, Xp, Ze
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Smad3 Antibody Summary

• Immunogen: A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
• Predicted Species: Zebrafish (90%), Chicken (90%), Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMAD3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Western Blot 0.5 - 2 ug/mL
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Smad3 Antibody

• DKFZp586N0721
• DKFZp686J10186
• hMAD-3
• hSMAD3
• HsT17436
• JV15-2MGC60396
• MAD homolog 3
• mad protein homolog
• MAD, mothers against decapentaplegic homolog 3 (Drosophila)
• MAD, mothers against decapentaplegic homolog 3
• mad3
• MADH3mad homolog JV15-2
• mothers against decapentaplegic homolog 3
• Mothers against DPP homolog 3
• SMA- and MAD-related protein 3
• SMAD 3
• SMAD family member 3HSPC193
• SMAD, mothers against DPP homolog 3 (Drosophila)
• SMAD, mothers against DPP homolog 3
• Smad3

Background

SMAD3, a member of the SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain. It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. SMAD3 is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad3 Antibody (NB100-56479)(6)

Publications using NB100-56479

• Chen B, Li R, Hernandez S et al. Differential effects of Smad2 and Smad3 in regulation of macrophage phenotype and function in the infarcted myocardium Journal of Molecular and Cellular Cardiology 2022-07-01 [PMID: 35780861]
• Wu X, Wang H, Chen H et al. Overexpression of smad7 inhibits the TGF-beta/Smad signaling pathway and EMT in NPHP1-defective MDCK cells Biochem Biophys Res Commun 2021-12-10 [PMID: 34689106] (WB, ICC/IF, Canine)
• Li Y, Zhao S, Xu S et al. Palmitoylethanolamide (PEA) reduces postoperative adhesions after experimental strabismus surgery in rabbits by suppressing canonical and non-canonical TGF beta signaling through PPAR alpha Biochemical pharmacology 2020-12-29 [PMID: 33385371] (WB, Mouse)
• Chen SM, Hsu LJ, Lee HL et al. Lactobacillus Attenuate the Progression of Pancreatic Cancer Promoted by Porphyromonas Gingivalis in K-rasG12D Transgenic Mice Cancers (Basel) 2020-11-26 [PMID: 33255941] (IHC-P, Mouse)
  
  Details:
  Pancreas samples from transgenic mice exposed to P. gingivalis underwent immunohistochemical analysis.
• Sivadas VP, George NA, Kattoor J, Kannan S. Novel mutations and expression alterations in SMAD3/TGFBR2 genes in oral carcinoma correlate with poor prognosis. Genes Chromosomes Cancer 2013-11-01 [PMID: 23913824] (WB, Human)
• Ruebel KH, Leontovich AA, Tanizaki Y et al. Effects of TGFbeta1 on gene expression in the HP75 human pituitary tumor cell line identified by gene expression profiling. Endocrine. 2008-02-01 [PMID: 18401765] (WB, Human)

Reviews for Smad3 Antibody (NB100-56479) (5)

Average Rating: 3.2

(Based on 5 reviews)

We have 5 reviews tested in 4 species: Human, Mouse, Bovine, mice.

reviewed by: Ashleigh Dean WB Mouse 08/02/2021

Summary

• Application: Western Blot
• Sample Tested: Lung tissue
• Species: Mouse
• Lot: 07172507B-3

reviewed by: Ajay Ashok WB mice 05/26/2021

Summary

• Application: Western Blot
• Sample Tested: eye tissue
• Species: mice
• Lot: 07172507B-4

Comments

• Comments: used at 1:100 overnight.
  ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

reviewed by: Ajay Ashok WB Bovine 05/26/2021

Summary

• Application: Western Blot
• Sample Tested: eye tissue
• Species: Bovine
• Lot: 07172507B-4

Comments

• Comments: Used at 1:1000- for overnight.
  ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to that that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

reviewed by: Ajay Ashok WB Human 05/24/2021

Summary

• Application: Western Blot
• Sample Tested: ocular tissue
• Species: Human
• Lot: 07172507B-4

Comments

• Comments: used at 1:1000 and incubated at 4degrees overnight

reviewed by: Yun Chen WB Mouse 06/17/2019

Summary

• Application: Western Blot
• Sample Tested: Human fibroblast and HeLa whole cell lysate
• Species: Mouse
• Lot: img-5848a

Comments

• Comments: 1:1000 dilution
  1 hour blocking in milk 5%

FAQs for Smad3 Antibody (NB100-56479). (Showing 1 - 1 of 1 FAQs).

1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
   • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Bioinformatics

• Gene Symbol: SMAD3
• Entrez:
  • 17127(Mouse)
  • 25631(Rat)
• Uniprot:
  • NP_005893(Human)