Smad2/3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more
Smad2/3 was detected in immersion fixed MCF-7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific ...read more
Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 3 µg/mL for 1 ...read more
Smad2/3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) at 10 µg/mL ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Smad2/3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad3
Ser2-Ala230
Accession # P84022
Specificity
Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMAD2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3797 in the following applications:

Publications
Read Publications using
AF3797 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2/3 Antibody [Unconjugated]

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2MGC34440
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smad2/3

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad2/3 Antibody (AF3797)(24)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Xenopus.

We have publications tested in 7 applications: ChIP, Flow Cytometry, ICC, IHC, Immunoprecipitation, Simple Western, Western Blot.


Filter By Application
ChIP
(4)
Flow Cytometry
(1)
ICC
(1)
IHC
(2)
Immunoprecipitation
(2)
Simple Western
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(11)
Mouse
(5)
Rat
(1)
Xenopus
(1)
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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using AF3797 Applications Species
Militi, S;Nibhani, R;Jalali, M;Pauklin, S; RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling Cell reports 2023-09-18 [PMID: 37725511] (ICC, Human) ICC Human
Mouti MA, Deng S, Pook M et al. KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics Nature communications 9/14/2023 [PMID: 37709746]
Yoshida M, Kokubun T, Sato K et al. DPP-4 Inhibitors Attenuate Fibrosis After Glaucoma Filtering Surgery by Suppressing the TGF-?/Smad Signaling Pathway Investigative ophthalmology & visual science 7/3/2023 [PMID: 37405760]
RA Razali, MD Yazid, A Saim, RBH Idrus, Y Lokanathan Approaches in Hydroxytyrosol Supplementation on Epithelial-Mesenchymal Transition in TGFbeta1-Induced Human Respiratory Epithelial Cells International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835384] (Simple Western, Human) Simple Western Human
M Sisto, D Ribatti, G Ingravallo, S Lisi The Expression of Follistatin-like 1 Protein Is Associated with the Activation of the EMT Program in Sj�gren's Syndrome Journal of Clinical Medicine, 2022-09-13;11(18):. 2022-09-13 [PMID: 36143013] (Western Blot, Human) Western Blot Human
Rebecca L. Mather, Abhijit Parolia, Sandra E. Carson, Erik Venalainen, David Roig‐Carles, Mustapha Jaber et al. The evolutionarily conserved long non‐coding RNA LINC00261 drives neuroendocrine prostate cancer proliferation and metastasis via distinct nuclear and cytoplasmic mechanisms Molecular Oncology 7/1/2021 [PMID: 33793068]
LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human) Western Blot Human
Chen PY, Qin L, Li G et al. Smooth Muscle Cell Reprogramming in Aortic Aneurysms Cell Stem Cell 4/2/2020 [PMID: 32243809]
L Wang, Y Li, X Wang, P Wang, K Essandoh, S Cui, W Huang, X Mu, Z Liu, Y Wang, T Peng, GC Fan GDF3 Protects Mice against Sepsis-Induced Cardiac Dysfunction and Mortality by Suppression of Macrophage Pro-Inflammatory Phenotype Cells, 2020-01-03;9(1):. 2020-01-03 [PMID: 31947892] (Mouse) Mouse
M Sisto, L Lorusso, G Ingravallo, D Ribatti, S Lisi TGFbeta1-Smad canonical and -Erk noncanonical pathways participate in interleukin-17-induced epithelial-mesenchymal transition in Sj�gren's syndrome Lab. Invest., 2020-01-10;100(6):824-836. 2020-01-10 [PMID: 31925325] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
Show All 24 Publications.

Review for Smad2/3 Antibody (AF3797) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3797:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22868230
SpeciesMouse

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Verified Customer
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SMAD2
Uniprot