Smad2/3 Antibody [Unconjugated]

Images

 
Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. ...read more
Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific ...read more
Smad2/3 was detected in immersion fixed MCF‑7 human breast cancer cell line induced (upper panel) or non-induced (lower panel) to undergo epithelial-mesenchymal transition (EMT) using Goat Anti-Human/Mouse Smad2/3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Smad2/3 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad3
Ser2-Ala230
Accession # P84022
Specificity
Detects human, mouse, and rat Smad2/3 in Western blots. Predicted to detect rat based on sequence homology.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SMAD2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 25 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3797 in the following applications:

Publications
Read Publications using
AF3797 in the following applications:

  • 3 publications
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad2/3 Antibody [Unconjugated]

  • hMAD-2
  • hSMAD2
  • JV18
  • JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
  • MAD homolog 2
  • Mad protein homolog
  • Mad, mothers against decapentaplegic homolog 2
  • MADH2MGC34440
  • MADR2MGC22139
  • Mad-related protein 2
  • mother against DPP homolog 2
  • mothers against decapentaplegic homolog 2
  • Mothers against DPP homolog 2
  • Sma- and Mad-related protein 2
  • SMAD 2
  • SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
  • SMAD, mothers against DPP homolog 2
  • Smad2
  • Smad2/3

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77836
Species: Hu, Mu, Rt, Po, Bv, Ch, Xp, Ze
Applications: WB, ELISA, Flow, IHC, IHC-P, Single Cell Western
AF2097
Species: Hu
Applications: WB, ChIP, ICC
MAB2029
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC
AF3025
Species: Hu
Applications: WB, ELISA, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-56656
Species: Hu, Mu, Rt
Applications: WB
NBP2-45808
Species: Hu
Applications: IHC
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-19773
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
MAB17761
Species: Hu, Mu, Rt
Applications: WB, ICC

Publications for Smad2/3 Antibody (AF3797)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ChIP, IHC, IP, WB.


Filter By Application
ChIP
(3)
IHC
(1)
IP
(1)
WB
(3)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF3797 Applications Species
Tao Cheng Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-?1 to -?3 Translation Am. J. Pathol, 2016;0(0):. 2016 [PMID: 27939132] (WB,, Mouse) WB Mouse
Potential mechanisms underlying ectodermal differentiation of Wharton's jelly mesenchymal stem cells Biochem Biophys Res Commun, 2016;0(0):. 2016 [PMID: 27501759] (WB,, Human) WB Human
Yoon, Se-Jin, Foley, Joseph W, Baker, Julie C HEB associates with PRC2 and SMAD2/3 to regulate developmental fates. Nat Commun, 2015;6(0):6546. 2015 [PMID: 25775035] (ChIP,, Mouse) ChIP Mouse
Rosa A, Papaioannou M, Krzyspiak J, Brivanlou A miR-373 is regulated by TGFbeta signaling and promotes mesendoderm differentiation in human Embryonic Stem Cells. Dev Biol, 2014;391(1):81-8. 2014 [PMID: 24709321] (ChIP,, Human) ChIP Human
Pereira P, Dobreva M, Maas E, Cornelis F, Moya I, Umans L, Verfaillie C, Camus A, de Sousa Lopes S, Huylebroeck D, Zwijsen A Antagonism of Nodal signaling by BMP/Smad5 prevents ectopic primitive streak formation in the mouse amnion. Development, 2012;139(18):3343-54. 2012 [PMID: 22912414] (IP,, Human) IP Human
Kudryashova E, Kramerova I, Spencer MJ Satellite cell senescence underlies myopathy in a mouse model of limb-girdle muscular dystrophy 2H. J. Clin. Invest., 2012;122(5):1764-76. 2012 [PMID: 22505452] (WB,, Mouse) WB Mouse
Kim SW, Yoon SJ, Chuong E, Oyolu C, Wills AE, Gupta R, Baker J Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs. Dev. Biol., 2011;357(2):492-504. 2011 [PMID: 21741376] (ChIP,, Human) ChIP Human
Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension. Eur. Respir. J., 2007;29(6):1094-104. 2007 [PMID: 17392319] (IHC,, Rat) IHC Rat

Review for Smad2/3 Antibody (AF3797) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3797:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22868230
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22868230

Details

Detection NotesSee PMID 22868230

Comments

CommentsPublished: See PMID 22868230Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smad2/3 Antibody (AF3797) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array AF3797

Bioinformatics Tool for Smad2/3 Antibody (AF3797)

Discover related pathways, diseases and genes to Smad2/3 Antibody (AF3797). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Smad2/3 Antibody (AF3797)

Discover more about diseases related to Smad2/3 Antibody (AF3797).
 

Pathways for Smad2/3 Antibody (AF3797)

View related products by pathway.

PTMs for Smad2/3 Antibody (AF3797)

Learn more about PTMs related to Smad2/3 Antibody (AF3797).

Blogs on Smad2/3

There are no specific blogs for Smad2/3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SMAD2
Uniprot