Cyr61/CCN1 Antibody [Unconjugated]


Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with more
Cyr61/CCN1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using 15 µg/mL Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) overnight at more
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Cyr61/CCN1 at approximately 51 kDa (as indicated) using 10 more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Simple Western, IHC

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Catalog# & Formulation Size Price

Cyr61/CCN1 Antibody [Unconjugated] Summary

E. coli-derived recombinant mouse Cyr61/CCN1
Accession # NP_034646
Detects mouse Cyr61/CCN1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant human Cyr61/CCN1.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF4055 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cyr61/CCN1 Antibody [Unconjugated]

  • CCN family member 1
  • CCN1
  • CCN1IGF-binding protein 10
  • Cyr61
  • Cysteine-rich angiogenic inducer 61
  • cysteine-rich, angiogenic inducer, 61
  • GIG1
  • GIG1cysteine-rich heparin-binding protein 61
  • IBP-10
  • IGFBP-10
  • IGFBP10cysteine-rich, anigogenic inducer, 61
  • Insulin-like growth factor-binding protein 10
  • protein CYR61
  • Protein GIG1


Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated protein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176‑281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cyr61/CCN1 Antibody (AF4055)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Direct ELISA, IHC-Fr, Immunoprecipitation, Neutralization, Western Blot.

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Showing Publications 1 - 8 of 8.
Publications using AF4055 Applications Species
Menger, MM;Bauer, D;Bleimehl, M;Scheuer, C;Braun, BJ;Herath, SC;Rollmann, MF;Menger, MD;Laschke, MW;Histing, T; Sildenafil, a phosphodiesterase-5 inhibitor, stimulates angiogenesis and bone regeneration in an atrophic non-union model in mice Journal of translational medicine 2023-09-08 [PMID: 37684656] (Mouse) Mouse
N Cheng, KH Kim, LF Lau Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2 JCI Insight, 2022-06-16;0(0):. 2022-06-16 [PMID: 35708907] (Western Blot, Mouse) Western Blot Mouse
MM Menger, B Merscher, C Scheuer, BJ Braun, SC Herath, MF Rollmann, D Stenger, T Später, T Pohlemann, MD Menger, T Histing Amlodipine accelerates bone healing in a stable closed femoral fracture model in mice European cells & materials, 2021-05-24;41(0):592-602. 2021-05-24 [PMID: 34027631] (Western Blot, Mouse) Western Blot Mouse
D Feng, C Ngov, N Henley, N Boufaied, C Gerarduzzi Characterization of Matricellular Protein Expression Signatures in Mechanistically Diverse Mouse Models of Kidney Injury Sci Rep, 2019-11-13;9(1):16736. 2019-11-13 [PMID: 31723159] (Western Blot, Mouse) Western Blot Mouse
CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56. 2016-08-01 [PMID: 27482114] (Direct ELISA, IHC-Fr, Neutralization, Mouse) Direct ELISA, IHC-Fr, Neutralization Mouse
Kim K, Chen C, Alpini G, Lau L CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB. J Clin Invest, 2015-03-30;125(5):1886-900. 2015-03-30 [PMID: 25822023] (Western Blot, Mouse) Western Blot Mouse
Zhang F, Hao F, An D, Zeng L, Wang Y, Xu X, Cui M The matricellular protein Cyr61 is a key mediator of platelet-derived growth factor-induced cell migration. J Biol Chem, 2015-01-26;290(13):8232-42. 2015-01-26 [PMID: 25623072] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Kim K, Chen C, Monzon R, Lau L Matricellular protein CCN1 promotes regression of liver fibrosis through induction of cellular senescence in hepatic myofibroblasts. Mol Cell Biol, 2013-03-18;33(10):2078-90. 2013-03-18 [PMID: 23508104] (Western Blot, Mouse) Western Blot Mouse

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Gene Symbol CYR61