NOV/CCN3 Antibody

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NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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NOV/CCN3 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3
Thr32-Met357
Accession # P48745
Specificity
Detects human NOV/CCN3 in ELISAs and Western blots. In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOV
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture:Human NOV/CCN3 Antibody (Catalog # AF1640)
ELISA Detection:Human NOV/CCN3 Biotinylated Antibody (Catalog # BAF1640)
Standard: Recombinant Human NOV/CCN3 (Catalog # 1640-NV)
Control
NOV/CCN3 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF1640 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NOV/CCN3 Antibody

  • CCN family member 3
  • CCN3
  • CCN3IGF-binding protein 9
  • IBP-9
  • IGFBP-9
  • IGFBP9novH
  • Insulin-like growth factor-binding protein 9
  • nephroblastoma overexpressed gene
  • Nephroblastoma-overexpressed gene protein homolog
  • NOV
  • NOVH
  • protein NOV homolog

Background

NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1-4). NOV/CCN3 contains an N‑terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38-50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1-4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C‑terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and  alpha 5 beta 1 mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).

  1. Perbal, B. (2004) Lancet 363:62.
  2. Perbal, B. (2006) Cell Commun. Signal. 4:3.
  3. Martinerie, C. et al. (1994) Oncogene 9:2729.
  4. Snaith, M.R. et al. (1996) Genomics 38:425.
  5. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
  6. Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
  7. Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
  8. Li, C.L. et al. (2002) Mol. Pathol. 55:250.
  9. Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
  10. Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
  11. Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
  12. Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NOV/CCN3 Antibody (AF1640)(3)

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Video Protocols

WB Video Protocol

FAQs for NOV/CCN3 Antibody (AF1640). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for the expression of CCN3/NOV in tumor cells and I am interested in finding an antibody that has a lot of data to support the antibody is working well. Can you make a recommendation?
    • We have two antibodies, NBP1-88154 and NBP1-88155 that have been extremely well characterized by testing at the Human Protein Atlas. Please see the Human Protein Atlas website for full testing for these products.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF1640

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