Plexin A1 Antibody [Unconjugated]


Western blot shows lysates of mouse embryonic heart tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) followed by HRP-conjugated Anti-Goat more
Plexin A1 was detected in immersion fixed frozen sections of embryonic mouse spinal cord using Mouse Plexin A1 Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 15 µg/mL overnight at 4 °C. Tissue was stained more
Plexin A1 was detected in immersion fixed immature human dendritic cells using Goat Anti-Mouse Plexin A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4309) at 10 µg/mL for 3 hours at room temperature. more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF

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Plexin A1 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse Plexin A1
Accession # P70206
Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Plexin A2 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF4309 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin A1 Antibody [Unconjugated]

  • NOV
  • NOVP
  • Plexin A1
  • PLXN1
  • PLXNA1


Plexin A1 (formerly Plexin 1) is a 200 kDa type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). Plexin signaling induces cytoskeletal remodeling, which mediates cell migration and axon repulsion (2). The mouse Plexin A1 cDNA encodes 1894 amino acids (aa) including a 27 aa signal sequence, a 1215 aa extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain (1). Within the ECD, human Plexin A1 shares 95%, 95%, 92%, 80% and 79% aa sequence identity with mouse, rat, bovine, chicken and Xenopus Plexin A1, respectively. The four mouse Plexin A molecules share 59‑67% aa identity with each other. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2, and binds transmembrane Semaphorin 6D directly (3‑5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5‑8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12).

  1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
  2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
  3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
  4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
  5. Takahashi, T. et al. (1999) Cell 99:59.
  6. Yoshida, Y. et al. (2006) Neuron 52:775.
  7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
  8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
  9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
  10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
  11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
  12. Catalano, A. et al. (2009) Cancer Res. 69:1485.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Plexin A1 Antibody (AF4309)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC, IHC, Western Blot.

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Showing Publications 1 - 5 of 5.
Publications using AF4309 Applications Species
E Ivakhnitsk, MR Chin, D Siegel, VH Guaiquil Vinaxanthone inhibits Semaphorin3A induced axonal growth cone collapse in embryonic neurons but fails to block its growth promoting effects on adult neurons Scientific Reports, 2021;11(1):13019. 2021 [PMID: 34155284] (ICC, Human) ICC Human
MS Jahan, T Ito, S Ichihashi, T Masuda, MER Bhuiyan, I Takahashi, H Takamatsu, A Kumanogoh, T Tsuzuki, T Negishi, K Yukawa PlexinA1 deficiency in BALB/cAJ mice leads to excessive self-grooming and reduced prepulse inhibition IBRO Reports, 2020;9(0):276-289. 2020 [PMID: 33163687] (Western Blot, Mouse) Western Blot Mouse
N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (Western Blot, Mouse) Western Blot Mouse
MM Hossain, T Tsuzuki, K Sakakibara, F Imaizumi, A Ikegaya, M Inagaki, I Takahashi, T Ito, H Takamatsu, A Kumanogoh, T Negishi, K Yukawa PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice PLoS ONE, 2019;14(8):e0221440. 2019 [PMID: 31430342] (Western Blot, Mouse) Western Blot Mouse
JS Jung, KD Zhang, Z Wang, M McMurray, A Tkaczuk, Y Ogawa, R Hertzano, TM Coate Semaphorin-5B Controls Spiral Ganglion Neuron Branch Refinement During Development J. Neurosci., 2019;0(0):. 2019 [PMID: 31209173] (IHC, Mouse) IHC Mouse

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Gene Symbol PLXNA1