Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free [NBP3-25417] - Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (NBP3-25417) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2048 + HL1907
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Collagen I/II/III.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COL1A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Collagen I/II/III Antibody (HL2048 + HL1907) - Azide and BSA Free

• Alpha-1 type I collagen
• collagen alpha 1 chain type I
• collagen alpha-1(I) chain
• collagen of skin, tendon and bone, alpha-1 chain
• collagen, type I, alpha 1
• OI4
• pro-alpha-1 collagen type 1

Background

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL1A1