CD44 Antibody (MEM-263)

Images

 
Western Blot: CD44 Antibody (MEM-263) [NB500-481] - Fig. 1. Western Blotting analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263). Lane 1: lysate of ...read more
Immunocytochemistry/ Immunofluorescence: CD44 Antibody (MEM-263) [NB500-481] - analysis of CD44 in 4% paraformaldehyde fixed Caov-3 cells (adherent human ovarian cancer cells) using anti-Cd44 antibody. The primary ...read more
Flow Cytometry: CD44 Antibody (MEM-263) [NB500-481] - A surface stain was performed on hPBMCs with CD44 (MEM-263) antibody NB500-481AF647 (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were ...read more

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
MEM-263
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CD44 Antibody (MEM-263) Summary

Immunogen
COS-7 cells, African Green Monkey (NM_000610.3).
Marker
Cell Membrane Marker
Specificity
The antibody MEM-263 reacts with extracellular (N-terminal) domain of standard CD44 (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. HLDA III; WS Code T 155
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry 4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (6% separating gel). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25481699)
Reviewed Applications
Read 1 Review rated 4
using
NB500-481 in the following applications:

Publications
Read Publications using
NB500-481 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD44 Antibody (MEM-263)

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (NB500-481)(5)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 2 applications: FLOW, ICC/IF.


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FLOW
(2)
ICC/IF
(1)
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Human
(1)
Porcine
(2)
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Review for CD44 Antibody (NB500-481) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB500-481:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence CD44 NB500-481
Enlarge
reviewed by:
Joelle Sacks
IF Human 12/16/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedadherent human ovarian cancer cells
SpeciesHuman
Lot518833

Blocking

Blocking Details10% goat serum in PBS 1 hour room temperature

Primary Anitbody

Dilution Ratio1:1000 in 2% goat serum in PBS for 2 hours room temperature

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, Alexa Fluor 488
Secondary Manufacturer Cat#Life Technologies #A11029
Secondary Concentration1:2000, 1 hour room temperature

Details

Detection NotesZeiss Axioskop2 fluorescence microscope, using Axiovision 3.0 software, 284ms exposure
Fixation Details4% paraformaldehye fixation 20 mins room temperature
Wash Description3 washes with PBS 3 mins each

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD44 Antibody (NB500-481) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-481AF405
Alexa Fluor 488 Labeled NB500-481AF488
Alexa Fluor 647 Labeled NB500-481AF647
Alexa Fluor 700 Labeled NB500-481AF700
Allophycocyanin Labeled NB500-481APC
DyLight 350 Labeled NB500-481UV
DyLight 405 Labeled NB500-481V
DyLight 488 Labeled NB500-481G
DyLight 680 Labeled NB500-481FR
DyLight 755 Labeled NB500-481IR
FITC Labeled NBP1-42789
PE Labeled NB500-481PE
PerCP Labeled NB500-481PCP

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Joelle Sacks
12/16/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol CD44
Entrez
Uniprot