CD31/PECAM-1 Antibody (BLR127H)

Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Whole cell lysate (10 ug) from MOLT-4, HEK293T, U2OS, Jurkat, A-549, GaMG, MCF-7, and HUVEC-C cells prepared using NETN lysis buffer. Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Rabbit anti- CD31/PECAM-1 recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] -: Rabbit antiCD31/PECAM-1 recombinant monoclonal antibody[BLR127H]. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR127H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD31/PECAM-1 Antibody (BLR127H) Summary

• Additional Information: Recombinant Monoclonal
• Immunogen: residues 28-601 (ECD)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PECAM1
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100 to 1:500
  • Immunohistochemistry 1:100 to 1:500
  • Immunohistochemistry-Paraffin 1:100 to 1:500
  • Immunoprecipitation 6 µl/1 mg lysate
  • Western Blot 1:1,000
• Application Notes: 0. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for CD31/PECAM-1 Antibody (BLR127H)

• adhesion molecule
• CD31 antigen
• CD31
• CD31/EndoCAM
• EndoCAM
• FLJ34100
• FLJ58394
• GPIIA'
• PECA1
• PECAM1
• PECAM-1
• PECAM-1, CD31/EndoCAM
• platelet endothelial cell adhesion molecule
• platelet endothelial cell adhesion molecule-1
• platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PECAM1