CXCR4 Antibody (44716) [Unconjugated]

Images

 
CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with ...read more
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), ...read more
CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the ...read more
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, B/N, CyTOF-ready
Clone
44716
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

CXCR4 Antibody (44716) [Unconjugated] Summary

Immunogen
3T3 cells transfected with human CXCR4
Specificity
Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CXCR4
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Neutralization 2.5-12 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB172 in the following applications:

Publications
Read Publications using
MAB172 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (44716) [Unconjugated]

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HM89NPYRL
  • HSY3RR
  • LAP3
  • LCR1
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPY3RWHIM
  • NPYR
  • NPYY3R
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor

Background

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).
  1. Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
  2. Zagzag, D. et al. (2005) Cancer Res. 65:6178.
  3. Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
  4. Wang, L. et al. (2009) Oncology Reports 22:1333.
  5. Amara, S. et al. (2015) Cancer Biomark. 15:869.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR4 Antibody (MAB172)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 12 applications: Flow, Functional Assay, ICC, IHC, IHC Fresh, IHC Fresh/Frozen, IHC Frozen, IHC Paraffin-embedded, IHC-P, IP, Neut, WB.


Filter By Application
Flow
(5)
Functional Assay
(1)
ICC
(8)
IHC
(2)
IHC Fresh
(2)
IHC Fresh/Frozen
(1)
IHC Frozen
(2)
IHC Paraffin-embedded
(6)
IHC-P
(1)
IP
(1)
Neut
(2)
WB
(1)
All Applications
Filter By Species
Human
(30)
Mouse
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using MAB172 Applications Species
W Tang, Y Chen, X Wang, Y Chen, J Zhang, Z Lin Expression of CXC-motif-chemokine 12 and the receptor C-X-C receptor 4 in glioma and theeffect on peritumoral brain edema Oncol Lett, 2018;15(2):2501-2507. 2018 [PMID: 29434965] (IHC-P,, Human) IHC-P Human
J Zhang, C Liu, X Mo, H Shi, S Li Mechanisms by which CXCR4/CXCL12 cause metastatic behavior in pancreatic cancer Oncol Lett, 2018;15(2):1771-1776. 2018 [PMID: 29434873] (IHC,, Human) IHC Human
M Fareh, F Almairac, L Turchi, F Burel-Vand, P Paquis, D Fontaine, S Lacas-Gerv, MP Junier, H Chneiweiss, T Virolle Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth Cell Death Dis, 2017;8(3):e2713. 2017 [PMID: 28358371] (WB,, Human) WB Human
Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27543332] (IHC Fresh,, Human) IHC Fresh Human
CJ Coke, KA Scarlett, MA Chetram, KJ Jones, BJ Sandifer, AS Davis, AI Marcus, CV Hinton Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression J. Biol. Chem., 2016;291(19):9991-10005. 2016 [PMID: 26841863] (Neut,, Human) Neut Human
Pilling D, Cox N, Vakil V, Verbeek J, Gomer R The long pentraxin PTX3 promotes fibrocyte differentiation. PLoS ONE, 2015;10(3):e0119709. 2015 [PMID: 25774777] (IHC Fresh,, Human) IHC Fresh Human
Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro. PLoS ONE, 2014;9(6):e99056. 2014 [PMID: 24897301] (ICC,, Human) ICC Human
Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res, 2014;20(13):3422-33. 2014 [PMID: 24763614] (IHC,, Human) IHC Human
Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms. PLoS ONE, 2014;9(4):e93965. 2014 [PMID: 24713821] (Flow,, Human) Flow Human
Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael An important role of the SDF-1/CXCR4 axis in chronic skin inflammation. PLoS ONE, 2014;9(4):e93665. 2014 [PMID: 24695674] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Show All 33 Publications.

Review for CXCR4 Antibody (MAB172) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB172:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry CXCR4 MAB172
Enlarge
reviewed by:
Anonymous
Flow Human 03/09/2018
View

Summary

ApplicationFlow Cytometry
Sample TestedJurkat
SpeciesHuman
LotAVB1116121

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CXCR4 Antibody (MAB172). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CXCR4 Antibody (MAB172)

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Blogs on CXCR4.

LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.

CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.

Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.

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Recent Reviews

5
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1
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2
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1
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Anonymous
03/09/2018
Application: Flow
Species: Human

Bioinformatics

Gene Symbol CXCR4
Entrez