CXCR4 Antibody (44716) [Unconjugated]


Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells more
CXCR4 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172) at 15 µg/mL overnight at 4 °C. Before incubation with more
CXCR4 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Human CXCR4 Monoclonal Antibody (Catalog # MAB172) overnight at 4 °C. Tissue was stained with the more
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human CXCR4 Monoclonal Antibody (Catalog # MAB172, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), more

Product Details

Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, Neut

CXCR4 Antibody (44716) [Unconjugated] Summary

3T3 cells transfected with human CXCR4
Detects human CXCR4. It will also react with cells expressing feline CXCR4 but not rat CXCR4.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
  • Neutralization 2.5-12 ug/mL
Read Publications using
MAB172 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR4 Antibody (44716) [Unconjugated]

  • CD184
  • chemokine (C-X-C motif) receptor 4
  • chemokine (C-X-C motif), receptor 4 (fusin)
  • C-X-C chemokine receptor type 4
  • CXCR4
  • CXC-R4
  • CXCR-4
  • D2S201ESDF-1 receptor
  • FB22
  • Fusin
  • HSY3RR
  • LAP3
  • LCR1
  • LESTRCD184 antigen
  • Leukocyte-derived seven transmembrane domain receptor
  • leukocyte-derived seven-transmembrane-domain receptor
  • lipopolysaccharide-associated protein 3
  • neuropeptide Y receptor Y3
  • NPYR
  • NPYY3R
  • seven transmembrane helix receptor
  • seven-transmembrane-segment receptor, spleen
  • Stromal cell-derived factor 1 receptor


CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCR4 Antibody (MAB172-100)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 11 applications: Flow, Functional Assay, ICC, IHC, IHC Fresh, IHC Fresh/Frozen, IHC Frozen, IHC Paraffin-embedded, IP, Neut, WB.

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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using MAB172 Applications Species
M Fareh, F Almairac, L Turchi, F Burel-Vand, P Paquis, D Fontaine, S Lacas-Gerv, MP Junier, H Chneiweiss, T Virolle Cell-based therapy using miR-302-367 expressing cells represses glioblastoma growth Cell Death Dis, 2017;8(3):e2713. 2017 [PMID: 28358371] (WB,, Human) WB Human
Signal transmission through the CXC chemokine receptor 4 (CXCR4) transmembrane helices Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27543332] (IHC Fresh,, Human) IHC Fresh Human
CJ Coke, KA Scarlett, MA Chetram, KJ Jones, BJ Sandifer, AS Davis, AI Marcus, CV Hinton Simultaneous Activation of Induced Heterodimerization between CXCR4 Chemokine Receptor and Cannabinoid Receptor 2 (CB2) Reveals a Mechanism for Regulation of Tumor Progression J. Biol. Chem., 2016;291(19):9991-10005. 2016 [PMID: 26841863] (Neut,, Human) Neut Human
Pilling D, Cox N, Vakil V, Verbeek J, Gomer R The long pentraxin PTX3 promotes fibrocyte differentiation. PLoS ONE, 2015;10(3):e0119709. 2015 [PMID: 25774777] (IHC Fresh,, Human) IHC Fresh Human
Hu, Weidong, Liu, Yue, Zhou, Wenhui, Si, Lianlian, Ren, Liang CXCL16 and CXCR6 are coexpressed in human lung cancer in vivo and mediate the invasion of lung cancer cell lines in vitro. PLoS ONE, 2014;9(6):e99056. 2014 [PMID: 24897301] (ICC,, Human) ICC Human
Hartmann N, Giese N, Giese T, Poschke I, Offringa R, Werner J, Ryschich E Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer. Clin Cancer Res, 2014;20(13):3422-33. 2014 [PMID: 24763614] (IHC,, Human) IHC Human
Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms. PLoS ONE, 2014;9(4):e93965. 2014 [PMID: 24713821] (Flow,, Human) Flow Human
Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael An important role of the SDF-1/CXCR4 axis in chronic skin inflammation. PLoS ONE, 2014;9(4):e93665. 2014 [PMID: 24695674] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node. J Immunol, 2014;192(5):2133-42. 2014 [PMID: 24493818] (Functional Assay,, Human) Functional Assay Human
Hoque, Md Tamji, Windus, Louisa C, Lovitt, Carrie J, Avery, Vicky M PCaAnalyser: a 2D-image analysis based module for effective determination of prostate cancer progression in 3D culture. PLoS ONE, 2013;8(11):e79865. 2013 [PMID: 24278197] (ICC,, Human) ICC Human
Show All 31 Publications.

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Product General Protocols

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FAQs for CXCR4 Antibody (MAB172-100). (Showing 1 - 1 of 1 FAQs).

  1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
    • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Secondary Antibodies


Isotype Controls

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Bioinformatics Tool for CXCR4 Antibody (MAB172-100)

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Blogs on CXCR4.

LAMP2: Protector of the lysosome
LAMP2 belongs to the family of membrane glycoproteins who confer selectins with carbohydrate ligands. LAMP2 has been implicated in tumor cell metastasis, as well as overall protection, maintenance, and adhesion of the lysosome. It appears that LAMP2 m...  Read full blog post.

CXCR4 Studies on Neural and Stem Cells
The CXCR4 (C-X-C chemokine receptor type 4) protein is one member of the G-protein coupled receptor (GPCR1) family. As a multipass membrane protein that is found in several tissues, it is the receptor for the C-X-C chemokine CXCL12/SDF-1. The CXCR4 li...  Read full blog post.

Understanding CXCR4 and SDF1
CXCR4 (C-X-C chemokine receptor type 4) is a member of the G-protein coupled receptor (GPCR1) family. It is expressed as a multipass membrane protein in several tissues where it acts as the receptor for the C-X-C chemokine CXCL12/SDF-1. This ligand in...  Read full blog post.

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Gene Symbol CXCR4