ABCG2/CD338 Antibody (3G8) - BSA Free

Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.

Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.

Flow Cytometry: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).

ELISA: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

• Reactivity: Hu, Mu, Pm
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

ABCG2/CD338 Antibody (3G8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human ABCG2 expressed in E. coli. [Uniprot: Q9UNQ0]
• Marker: Stem Cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCG2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Application Notes: In Western blot, a dimer can be seen at 60-70 kDa representing ABCG2.
• Theoretical MW: 60-70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for ABCG2/CD338 Antibody (3G8) - BSA Free

• ABC transporter
• ABC15
• ABCG2
• ABCP
• ABCPMGC102821
• ATP-binding cassette sub-family G member 2
• ATP-binding cassette transporter G2
• ATP-binding cassette, sub-family G (WHITE), member 2
• BCRP
• BCRP1
• BCRPMRX
• BMDP
• Breast cancer resistance protein
• CD338 antigen
• CD338
• CDw338
• EST157481
• mitoxantrone resistance protein
• Mitoxantrone resistance-associated protein
• MXR
• MXRMXR1
• placenta specific MDR protein
• Placenta-specific ATP-binding cassette transporter

Background

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG2/CD338 Antibody (NBP2-22124)(8)

Publications using NBP2-22124

• Fridman LB, Knerler S, Price AS et al. Cocaine Regulates Antiretroviral Therapy CNS Access Through Pregnane-X Receptor-Mediated Drug Transporter and Metabolizing Enzyme Modulation at the Blood Brain Barrier bioRxiv 2023-08-02 [PMID: 37546800] (WB, ICC/IF)
• Bhadwal P, Randhawa V, Vaiphei K et al. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance Scientific reports 2022-10-29 [PMID: 36309544] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Yamamoto S, Fukuhara H, Seki H Et Al. Predictors of therapeutic efficacy of 5-aminolevulinic acid-based photodynamic therapy in human prostate cancer Photodiagnosis and photodynamic therapy 2021-07-21 [PMID: 34303032] (IHC-P)
• Huang R, Zhu L, Zhang Y XIST lost induces ovarian cancer stem cells to acquire taxol resistance via a KMT2C-dependent way Cancer cell international 2020-09-04 [PMID: 32943985] (WB, Human)
• Sridharan S, Robeson M, Bastihalli-Tukaramrao D, et al. Targeting of the Eukaryotic Translation Initiation Factor 4A Against Breast Cancer Stemness Front Oncol 2019-12-06 [PMID: 31867270] (WB, Human)
• Erdogan S, Turkekul K, Dibirdik I et al. Midkine downregulation increases the efficacy of quercetin on prostate cancer stem cell survival and migration through PI3K/AKT and MAPK/ERK pathway Biomed. Pharmacother. 2018-08-21 [PMID: 30142541] (WB, Human)
• Agarwalla P, Mukherjee S, Sreedhar B, Banerjee R. Glucocorticoid receptor-mediated delivery of nano gold-withaferin conjugates for reversal of epithelial-to-mesenchymal transition and tumor regression. Nanomedicine (Lond) 2016-10-01 [PMID: 27622735]
• Mondal SK, Jinka S, Pal K et al. Glucocorticoid Receptor-Targeted Liposomal Codelivery of Lipophilic Drug and Anti-Hsp90 Gene: Strategy to Induce Drug-Sensitivity, EMT-Reversal, and Reduced Malignancy in Aggressive Tumors. Mol. Pharm. 2016-07-05 [PMID: 27184196] (WB)

Review for ABCG2/CD338 Antibody (NBP2-22124) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot ICC Human 11/16/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: A2058 human melanoma cell line
• Species: Human
• Lot: A-6

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Bioinformatics

• Gene Symbol: ABCG2
• Entrez:
  • 9429(Human)
• Uniprot:
  • Q9UNQ0(Human)