Western Blot: ABCG2 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in 1) human small intestine, 2) human placenta and 3) mouse placenta tissue extracts.
Immunocytochemistry/ Immunofluorescence: ABCG2 Antibody (3G8) [NBP2-22124] - Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry-Paraffin: ABCG2 Antibody (3G8) [NBP2-22124] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB ...read more
Flow Cytometry: ABCG2 Antibody (3G8) [NBP2-22124] - Flow cytometric analysis of HepG2 cells using ABCG2 mouse mAb (green) and negative control (purple).
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
Ammonium sulfate precipitation
This ABCG2 (3G8) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA. In WB a dimer can be seen at 60-70 kDa representing ABCG2.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.