Carbonic Anhydrase IX/CA9 Antibody (2D3)


Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Western blot analysis using Carbonic Anhydrase IX mouse mAb against Hela (1) and A549 (2) cell lysates.
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse more
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). more
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple).

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IHC, IHC-P
1.0 mg/ml

Carbonic Anhydrase IX/CA9 Antibody (2D3) Summary

Purified recombinant fragment of human Carbonic Anhydrase IX expressed in E. coli. [UniProt# Q16790]
Hypoxia Marker
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein A or G purified


Application Notes
This Carbonic Anhydrase IX (2D3) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa
Reviewed Applications
Read 1 Review rated 5
NBP1-51691 in the following application:

Read Publications using
NBP1-51691 in the following applications:

  • IHC
    1 publication
  • WB
    2 publications

Reactivity Notes


Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (2D3)

  • CA9
  • CA-IX
  • CAIXcarbonic anhydrase 9
  • Carbonate dehydratase IX
  • carbonic anhydrase IXpMW1
  • carbonic dehydratase
  • EC
  • G250
  • Membrane antigen MN
  • MN
  • MNRCC-associated antigen G250
  • P54/58N
  • RCC
  • RCC-associated protein G250
  • Renal cell carcinoma-associated antigen G250


Carbonic Anhydrase IX, also known as CA9, is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carbonic Anhydrase IX/CA9 Antibody (NBP1-51691)(3)

Reviews for Carbonic Anhydrase IX/CA9 Antibody (NBP1-51691) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-51691:
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Images Ratings Applications Species Date Details
Western Blot Carbonic Anhydrase IX/CA9 NBP1-51691
reviewed by:
WB Human 11/20/2015


ApplicationWestern Blot
Sample TestedMCF-7 whole cell lysate


Blocking DetailsPBST with 5% non-fat dry milk, 1 h, Room Temperature

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG HRP
Secondary Manufacturer Cat#sc-2005
Secondary Concentration1:2000


Detection NotesChemiluminescence, GBox Chemi XL1.4 from Syngene, 12 sec, no positive or negative controls


CommentsThis antibody can be used at much higher dilution. At lower dilution (less than 1:1000) or if high amount of protein is loaded multiple bands may be seen.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Carbonic Anhydrase IX/CA9 Antibody (NBP1-51691). (Showing 1 - 2 of 2 FAQs).

  1. We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?
    • We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.
  2. I am looking for a primary antibody to Carbonic Anhydrase 9 and I want an antibody that recognises an extracellular epitope so that I can use it for live cell flow cytometry. Would #NBP1-51691 be a suitable candidate?
    • The immunogen of our Carbonic Anhydrase IX antibody (clone 2D3) # NBP1-51691 corresponds to peptide from amino acids region between 40-290 of human CA9, which appears to be in the extracellular domain of this protein (based on UniProt: Q16790). In our QC lab, this antibody has been validated for FLOW application, so that you may use this antibody for your current experiments.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-51691AF405
Alexa Fluor (R) 488 Labeled NBP1-51691AF488
Alexa Fluor (R) 647 Labeled NBP1-51691AF647
Alexa Fluor (R) 700 Labeled NBP1-51691AF700
Allophycocyanin Labeled NBP1-51691APC
Biotin Labeled NBP1-51691B
DyLight 350 Labeled NBP1-51691UV
DyLight 405 Labeled NBP1-51691V
DyLight 405LS Labeled NBP1-51691V3
DyLight 488 Labeled NBP1-51691G
DyLight 550 Labeled NBP1-51691R
DyLight 650 Labeled NBP1-51691C
DyLight 680 Labeled NBP1-51691FR
DyLight 755 Labeled NBP1-51691IR
HRP Labeled NBP1-51691H
PerCP Labeled NBP1-51691PCP

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Blogs on Carbonic Anhydrase IX/CA9.

Carbonic anhydrase IX (CAIX) - a reliable histochemical marker of hypoxia
Carbonic anhydrase IX is a member of the carbonic anhydrase family. This family consists of catalytic enzymes capable of converting carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. This family of molecules is abundantly e...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol CA9

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