Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Western blot analysis using Carbonic Anhydrase IX mouse mAb against Hela (1) and A549 (2) cell lysates.
Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Immunohistochemical analysis of paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse ...read more
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of Biotin conjugate of NBP1-51691. Mouse choroid stained with anti-Carbonic Anhydrase IX antibody. Image from ...read more
Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). ...read more
Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple).
This Carbonic Anhydrase IX (2D3) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein A or G purified
Alternate Names for Carbonic Anhydrase IX/CA9 Antibody (2D3)
CAIXcarbonic anhydrase 9
Carbonate dehydratase IX
Carbonic Anhydrase IX
carbonic anhydrase IXpMW1
Membrane antigen MN
MNRCC-associated antigen G250
RCC-associated protein G250
Renal cell carcinoma-associated antigen G250
Carbonic Anhydrase IX, also known as CA9, is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Carbonic Anhydrase IX/CA9 Antibody (NBP1-51691). (Showing 1 - 2 of 2 FAQs).
We are looking for the antibody available for cell sorting by CA9. The sorted cells must be cultured for the expansion after the sorting for the following experiments. Do you have the antibodies useful for this?
We sell two carbonic anhydrase IX antibodies that have been validated for Flow Cytometry detection of the human protein. These have catalogue numbers NBP1-51691 and NBP1-47688 and can be seen at the following link: carbonic anhydrase IX antibodies. Since both of these products contain sodium azide as a preservative, you are unlikely to be able to culture the cells on following sorting unless you performed a buffer exchange prior to using the antibody. Unfortunately we have no direct experience with either of these products of staining and subsequently expanding cell populations.
I am looking for a primary antibody to Carbonic Anhydrase 9 and I want an antibody that recognises an extracellular epitope so that I can use it for live cell flow cytometry. Would #NBP1-51691 be a suitable candidate?
The immunogen of our Carbonic Anhydrase IX antibody (clone 2D3) # NBP1-51691 corresponds to peptide from amino acids region between 40-290 of human CA9, which appears to be in the extracellular domain of this protein (based on UniProt: Q16790). In our QC lab, this antibody has been validated for FLOW application, so that you may use this antibody for your current experiments.
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