Capicua Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Capicua Antibody [NB110-59905] - Capicua antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight ...read more
Immunoprecipitation: Capicua Antibody [NB110-59905] - Detection of Human CIC/Capicua by WB of IPs. Whole cell lysate from HeLa cells. Affinity purified rabbit NB110-59905 used for IP at 3 mcg/mg lysate. CIC was also ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications ICC/IF, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

Capicua Antibody - BSA Free Summary

Immunogen
The epitope recognized by this antibody maps to a region within residue 1150-1200 of human Capicua. [NP_055940.3]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CIC
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This Capicua antibody is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. It is not recommended for Western blot analysis. With ICC/IF nuclear staining was observed in Neuro2A cells.
Theoretical MW
164 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Capicua Antibody - BSA Free

  • capicua homolog (Drosophila)
  • KIAA0306capicua (Drosophila) homolog
  • protein capicua homolog

Background

Capicua is a transcriptional repressor protein belonging to HMG-box proteins which has been suggested to play a key role in CNS development and is expressed in foetal brain as well as medulloblastomas. Capicua protein expression levels are subject to posttranscriptional regulation by signaling downstream of two RTKs - EGFR and Torso (that propagate their signals through Ras-Raf-MAPK signaling cascade) and MAPK-mediated phosphorylation promotes capicua degradation. Capicua has been shown to act as a transcriptional repressor through interaction with a co-repressor Groucho. Moreover, Bantam which is a Hippo pathway player has recently been shown to acts in a feedback loop to limit Capicua expression for regulating EGFR signaling. Capicua is well conserved in mammals, and mediates RTK-dependent responses that are linked to cell proliferation, cell differentiation and cancer. Capicua can interact with ATXN1 as well as ATXN1L, and has been shown to be involved in Spinocerebellar Ataxia Type 1 (SCA-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for Capicua Antibody (NB110-59905): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Capicua Antibody (NB110-59905). (Showing 1 - 1 of 1 FAQ).

  1. Does this antibody work in Drosophila or Zebrafish?
    • I have checked the % homology between human capicua (which is what the Ab is raised to) and between drosophilia and Zebrafish capicua. The % homoloy with Drosophilia is only 27 % and our lab only recommends use if the % homology is 85% or above. The % homology with Zebrafish is only 42% so I would not recommend this either. Unfortunately all of our Capicua Anitbodies recognize the human protein therefore we do not have an antibody that would recognize Zebrafish or Drosophila Capicua.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CIC
Entrez
Uniprot